Antigenotoxicity and Tumor Growing Inhibition by Leafy Brassica carinata and Sinigrin

Cruciferous vegetables are well known and worldwide consumed due to their health benefits and cancer prevention properties. As a desirable cruciferous plant, Ethiopian mustard (Brassica carinata A. Braun) and its glucosinolate sinigrin were tested in the in vivo Drosophila melanogaster (SMART) and t...

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Autores principales: Lozano-Baena, María-Dolores, Tasset, Inmaculada, Obregón-Cano, Sara, de Haro-Bailon, Antonio, Muñoz-Serrano, Andrés, Alonso-Moraga, Ángeles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331809/
https://www.ncbi.nlm.nih.gov/pubmed/26343628
http://dx.doi.org/10.3390/molecules200915748
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author Lozano-Baena, María-Dolores
Tasset, Inmaculada
Obregón-Cano, Sara
de Haro-Bailon, Antonio
Muñoz-Serrano, Andrés
Alonso-Moraga, Ángeles
author_facet Lozano-Baena, María-Dolores
Tasset, Inmaculada
Obregón-Cano, Sara
de Haro-Bailon, Antonio
Muñoz-Serrano, Andrés
Alonso-Moraga, Ángeles
author_sort Lozano-Baena, María-Dolores
collection PubMed
description Cruciferous vegetables are well known and worldwide consumed due to their health benefits and cancer prevention properties. As a desirable cruciferous plant, Ethiopian mustard (Brassica carinata A. Braun) and its glucosinolate sinigrin were tested in the in vivo Drosophila melanogaster (SMART) and the in vitro HL60 (human promyelocytic leukaemia cell line) systems. High performance liquid chromatography (HPLC) analysis of plant samples confirmed the presence of sinigrin as principal B. carinata glucosinolate. SMART was performed by feeding D. melanogaster larvae either with different concentrations of plant/compound samples or combining them with hydrogen peroxide (a potent oxidative mutagen) being both antimutagenics. HL60 assays showed the tumoricidal activity of plant samples (IC(50) = 0.28 mg·mL(−1)) and the breakdown products of sinigrin hydrolysis (IC(50) = 2.71 µM). Our results enhance the potential of B. carinata as health promoter and chemopreventive in both systems and the leading role of sinigrin in these effects.
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spelling pubmed-63318092019-01-24 Antigenotoxicity and Tumor Growing Inhibition by Leafy Brassica carinata and Sinigrin Lozano-Baena, María-Dolores Tasset, Inmaculada Obregón-Cano, Sara de Haro-Bailon, Antonio Muñoz-Serrano, Andrés Alonso-Moraga, Ángeles Molecules Article Cruciferous vegetables are well known and worldwide consumed due to their health benefits and cancer prevention properties. As a desirable cruciferous plant, Ethiopian mustard (Brassica carinata A. Braun) and its glucosinolate sinigrin were tested in the in vivo Drosophila melanogaster (SMART) and the in vitro HL60 (human promyelocytic leukaemia cell line) systems. High performance liquid chromatography (HPLC) analysis of plant samples confirmed the presence of sinigrin as principal B. carinata glucosinolate. SMART was performed by feeding D. melanogaster larvae either with different concentrations of plant/compound samples or combining them with hydrogen peroxide (a potent oxidative mutagen) being both antimutagenics. HL60 assays showed the tumoricidal activity of plant samples (IC(50) = 0.28 mg·mL(−1)) and the breakdown products of sinigrin hydrolysis (IC(50) = 2.71 µM). Our results enhance the potential of B. carinata as health promoter and chemopreventive in both systems and the leading role of sinigrin in these effects. MDPI 2015-08-28 /pmc/articles/PMC6331809/ /pubmed/26343628 http://dx.doi.org/10.3390/molecules200915748 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lozano-Baena, María-Dolores
Tasset, Inmaculada
Obregón-Cano, Sara
de Haro-Bailon, Antonio
Muñoz-Serrano, Andrés
Alonso-Moraga, Ángeles
Antigenotoxicity and Tumor Growing Inhibition by Leafy Brassica carinata and Sinigrin
title Antigenotoxicity and Tumor Growing Inhibition by Leafy Brassica carinata and Sinigrin
title_full Antigenotoxicity and Tumor Growing Inhibition by Leafy Brassica carinata and Sinigrin
title_fullStr Antigenotoxicity and Tumor Growing Inhibition by Leafy Brassica carinata and Sinigrin
title_full_unstemmed Antigenotoxicity and Tumor Growing Inhibition by Leafy Brassica carinata and Sinigrin
title_short Antigenotoxicity and Tumor Growing Inhibition by Leafy Brassica carinata and Sinigrin
title_sort antigenotoxicity and tumor growing inhibition by leafy brassica carinata and sinigrin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331809/
https://www.ncbi.nlm.nih.gov/pubmed/26343628
http://dx.doi.org/10.3390/molecules200915748
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