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In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla

Swietenia macrophylla (mahogany) is a highly valued timber species, whereas the leaves are considered to be waste product. A total of 27 phenolic compounds were identified in aqueous extracts from mahogany leaves by comparing retention times and mass spectra data with those of authentic standards us...

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Autores principales: Pamplona, Sônia, Sá, Paulo, Lopes, Dielly, Costa, Edmar, Yamada, Elizabeth, e Silva, Consuelo, Arruda, Mara, Souza, Jesus, da Silva, Milton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331886/
https://www.ncbi.nlm.nih.gov/pubmed/26501245
http://dx.doi.org/10.3390/molecules201018777
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author Pamplona, Sônia
Sá, Paulo
Lopes, Dielly
Costa, Edmar
Yamada, Elizabeth
e Silva, Consuelo
Arruda, Mara
Souza, Jesus
da Silva, Milton
author_facet Pamplona, Sônia
Sá, Paulo
Lopes, Dielly
Costa, Edmar
Yamada, Elizabeth
e Silva, Consuelo
Arruda, Mara
Souza, Jesus
da Silva, Milton
author_sort Pamplona, Sônia
collection PubMed
description Swietenia macrophylla (mahogany) is a highly valued timber species, whereas the leaves are considered to be waste product. A total of 27 phenolic compounds were identified in aqueous extracts from mahogany leaves by comparing retention times and mass spectra data with those of authentic standards using LC-ESI-MS/MS. Polyphenols play an important role in plants as defense mechanisms against pests and pathogens and have potent antioxidant properties. In terms of health applications, interest has increased considerably in naturally occurring antioxidant sources, since they can retard the progress of many important neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases. The antioxidant capacities of two aqueous extracts, M1 (decoction) and M2 (infusion), were measured using TEAC and Folin-Ciocalteau methods. Additionally, M1 was used in order to investigate its potential cytoprotective effects on an in vitro model of neurodegeneration, by using primary cerebellar cultures exposed to methyl mercury (MeHg). Under experimental sub-chronic conditions (72 h), concomitant exposure of the same cultures to MeHg and M1 extract resulted in a statistically significant increase in cell viability in all three concentrations tested (10, 50 and 100 μg/mL), strongly suggesting that due to its high content of antioxidant compounds, the M1 extract provides significant cytoprotection against the MeHg-induced in vitro neurotoxicity.
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spelling pubmed-63318862019-01-24 In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla Pamplona, Sônia Sá, Paulo Lopes, Dielly Costa, Edmar Yamada, Elizabeth e Silva, Consuelo Arruda, Mara Souza, Jesus da Silva, Milton Molecules Article Swietenia macrophylla (mahogany) is a highly valued timber species, whereas the leaves are considered to be waste product. A total of 27 phenolic compounds were identified in aqueous extracts from mahogany leaves by comparing retention times and mass spectra data with those of authentic standards using LC-ESI-MS/MS. Polyphenols play an important role in plants as defense mechanisms against pests and pathogens and have potent antioxidant properties. In terms of health applications, interest has increased considerably in naturally occurring antioxidant sources, since they can retard the progress of many important neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases. The antioxidant capacities of two aqueous extracts, M1 (decoction) and M2 (infusion), were measured using TEAC and Folin-Ciocalteau methods. Additionally, M1 was used in order to investigate its potential cytoprotective effects on an in vitro model of neurodegeneration, by using primary cerebellar cultures exposed to methyl mercury (MeHg). Under experimental sub-chronic conditions (72 h), concomitant exposure of the same cultures to MeHg and M1 extract resulted in a statistically significant increase in cell viability in all three concentrations tested (10, 50 and 100 μg/mL), strongly suggesting that due to its high content of antioxidant compounds, the M1 extract provides significant cytoprotection against the MeHg-induced in vitro neurotoxicity. MDPI 2015-10-16 /pmc/articles/PMC6331886/ /pubmed/26501245 http://dx.doi.org/10.3390/molecules201018777 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pamplona, Sônia
Sá, Paulo
Lopes, Dielly
Costa, Edmar
Yamada, Elizabeth
e Silva, Consuelo
Arruda, Mara
Souza, Jesus
da Silva, Milton
In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla
title In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla
title_full In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla
title_fullStr In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla
title_full_unstemmed In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla
title_short In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla
title_sort in vitro cytoprotective effects and antioxidant capacity of phenolic compounds from the leaves of swietenia macrophylla
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331886/
https://www.ncbi.nlm.nih.gov/pubmed/26501245
http://dx.doi.org/10.3390/molecules201018777
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