Cargando…

Redox-Active Profile Characterization of Remirea maritima Extracts and Its Cytotoxic Effect in Mouse Fibroblasts (L929) and Melanoma (B16F10) Cells

Remirea maritima is a tropical plant with a reticulated root system belonging to the family Cyperaceae, also known to have biologically active secondary metabolites. However, very few data on R. maritima’s biological actions are available and there are no reports regarding the redox-active profile o...

Descripción completa

Detalles Bibliográficos
Autores principales: Dória, Grace Anne A., Santos, Anderson R., Bittencourt, Leonardo S., Bortolin, Rafael C., Menezes, Paula P., Vasconcelos, Bruno S., Souza, Rebeca O., Fonseca, Maria José V., Santos, Alan Diego C., Saravanan, Shanmugam, Silva, Francilene A., Gelain, Daniel P., Moreira, José Cláudio F., Prata, Ana Paula N., Quintans-Júnior, Lucindo J., Araújo, Adriano A. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331889/
https://www.ncbi.nlm.nih.gov/pubmed/26121396
http://dx.doi.org/10.3390/molecules200711699
_version_ 1783387221748875264
author Dória, Grace Anne A.
Santos, Anderson R.
Bittencourt, Leonardo S.
Bortolin, Rafael C.
Menezes, Paula P.
Vasconcelos, Bruno S.
Souza, Rebeca O.
Fonseca, Maria José V.
Santos, Alan Diego C.
Saravanan, Shanmugam
Silva, Francilene A.
Gelain, Daniel P.
Moreira, José Cláudio F.
Prata, Ana Paula N.
Quintans-Júnior, Lucindo J.
Araújo, Adriano A. S.
author_facet Dória, Grace Anne A.
Santos, Anderson R.
Bittencourt, Leonardo S.
Bortolin, Rafael C.
Menezes, Paula P.
Vasconcelos, Bruno S.
Souza, Rebeca O.
Fonseca, Maria José V.
Santos, Alan Diego C.
Saravanan, Shanmugam
Silva, Francilene A.
Gelain, Daniel P.
Moreira, José Cláudio F.
Prata, Ana Paula N.
Quintans-Júnior, Lucindo J.
Araújo, Adriano A. S.
author_sort Dória, Grace Anne A.
collection PubMed
description Remirea maritima is a tropical plant with a reticulated root system belonging to the family Cyperaceae, also known to have biologically active secondary metabolites. However, very few data on R. maritima’s biological actions are available and there are no reports regarding the redox-active profile of this plant. In this study, we examined the total phenolic content of Remirea maritima hydroalcoholic (RMHA) extracts, redox properties against different reactive species generated in vitro and their cytotoxic effect against fibroblasts (L929) and melanoma (B16F10) cells. Total reactive antioxidant potential index (TRAP) and total antioxidant reactivity (TAR) results revealed that RMHA at all concentrations tested showed significant antioxidant capacity. RMHA was also effective against hydroxyl radical formation, reduction of Fe(3+) to Fe(2+) and in scavenging nitric oxide (NO) radicals. In vitro, the level of lipid peroxidation was reduced by RMHA extract and the data showed significant oxidative damage protection. The RMHA cytotoxicity was evaluated by a neutral red assay in fibroblast (L929) and melanome (B16F10) cells. The obtained results showed that the RMHA (40 and 80 µg/mL, respectively) reduced 70% of the viable cells. In conclusion, this study represents the first report regarding the antioxidant and anti-proliferative potential of R. maritima against B16F10 melanoma cells.
format Online
Article
Text
id pubmed-6331889
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63318892019-01-24 Redox-Active Profile Characterization of Remirea maritima Extracts and Its Cytotoxic Effect in Mouse Fibroblasts (L929) and Melanoma (B16F10) Cells Dória, Grace Anne A. Santos, Anderson R. Bittencourt, Leonardo S. Bortolin, Rafael C. Menezes, Paula P. Vasconcelos, Bruno S. Souza, Rebeca O. Fonseca, Maria José V. Santos, Alan Diego C. Saravanan, Shanmugam Silva, Francilene A. Gelain, Daniel P. Moreira, José Cláudio F. Prata, Ana Paula N. Quintans-Júnior, Lucindo J. Araújo, Adriano A. S. Molecules Article Remirea maritima is a tropical plant with a reticulated root system belonging to the family Cyperaceae, also known to have biologically active secondary metabolites. However, very few data on R. maritima’s biological actions are available and there are no reports regarding the redox-active profile of this plant. In this study, we examined the total phenolic content of Remirea maritima hydroalcoholic (RMHA) extracts, redox properties against different reactive species generated in vitro and their cytotoxic effect against fibroblasts (L929) and melanoma (B16F10) cells. Total reactive antioxidant potential index (TRAP) and total antioxidant reactivity (TAR) results revealed that RMHA at all concentrations tested showed significant antioxidant capacity. RMHA was also effective against hydroxyl radical formation, reduction of Fe(3+) to Fe(2+) and in scavenging nitric oxide (NO) radicals. In vitro, the level of lipid peroxidation was reduced by RMHA extract and the data showed significant oxidative damage protection. The RMHA cytotoxicity was evaluated by a neutral red assay in fibroblast (L929) and melanome (B16F10) cells. The obtained results showed that the RMHA (40 and 80 µg/mL, respectively) reduced 70% of the viable cells. In conclusion, this study represents the first report regarding the antioxidant and anti-proliferative potential of R. maritima against B16F10 melanoma cells. MDPI 2015-06-25 /pmc/articles/PMC6331889/ /pubmed/26121396 http://dx.doi.org/10.3390/molecules200711699 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dória, Grace Anne A.
Santos, Anderson R.
Bittencourt, Leonardo S.
Bortolin, Rafael C.
Menezes, Paula P.
Vasconcelos, Bruno S.
Souza, Rebeca O.
Fonseca, Maria José V.
Santos, Alan Diego C.
Saravanan, Shanmugam
Silva, Francilene A.
Gelain, Daniel P.
Moreira, José Cláudio F.
Prata, Ana Paula N.
Quintans-Júnior, Lucindo J.
Araújo, Adriano A. S.
Redox-Active Profile Characterization of Remirea maritima Extracts and Its Cytotoxic Effect in Mouse Fibroblasts (L929) and Melanoma (B16F10) Cells
title Redox-Active Profile Characterization of Remirea maritima Extracts and Its Cytotoxic Effect in Mouse Fibroblasts (L929) and Melanoma (B16F10) Cells
title_full Redox-Active Profile Characterization of Remirea maritima Extracts and Its Cytotoxic Effect in Mouse Fibroblasts (L929) and Melanoma (B16F10) Cells
title_fullStr Redox-Active Profile Characterization of Remirea maritima Extracts and Its Cytotoxic Effect in Mouse Fibroblasts (L929) and Melanoma (B16F10) Cells
title_full_unstemmed Redox-Active Profile Characterization of Remirea maritima Extracts and Its Cytotoxic Effect in Mouse Fibroblasts (L929) and Melanoma (B16F10) Cells
title_short Redox-Active Profile Characterization of Remirea maritima Extracts and Its Cytotoxic Effect in Mouse Fibroblasts (L929) and Melanoma (B16F10) Cells
title_sort redox-active profile characterization of remirea maritima extracts and its cytotoxic effect in mouse fibroblasts (l929) and melanoma (b16f10) cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331889/
https://www.ncbi.nlm.nih.gov/pubmed/26121396
http://dx.doi.org/10.3390/molecules200711699
work_keys_str_mv AT doriagraceannea redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT santosandersonr redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT bittencourtleonardos redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT bortolinrafaelc redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT menezespaulap redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT vasconcelosbrunos redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT souzarebecao redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT fonsecamariajosev redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT santosalandiegoc redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT saravananshanmugam redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT silvafrancilenea redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT gelaindanielp redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT moreirajoseclaudiof redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT prataanapaulan redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT quintansjuniorlucindoj redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells
AT araujoadrianoas redoxactiveprofilecharacterizationofremireamaritimaextractsanditscytotoxiceffectinmousefibroblastsl929andmelanomab16f10cells