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In Vivo Antihyperglycemic Activity of a Lanosteryl Triterpene from Protorhus longifolia

Control of postprandial hyperglycemia is crucial in the management of diabetes mellitus. Despite the use of the current hypoglycemic drugs, incidence of diabetes and related diseases continue to increase. This study aimed at evaluating the in vivo antihyperglycemic activity of methyl-3β-hydroxylanos...

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Autores principales: Mosa, Rebamang A., Cele, Nkosinathi D., Mabhida, Sihle E., Shabalala, Samkelisiwe C., Penduka, Dambudzo, Opoku, Andy R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331905/
https://www.ncbi.nlm.nih.gov/pubmed/26205060
http://dx.doi.org/10.3390/molecules200713374
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author Mosa, Rebamang A.
Cele, Nkosinathi D.
Mabhida, Sihle E.
Shabalala, Samkelisiwe C.
Penduka, Dambudzo
Opoku, Andy R.
author_facet Mosa, Rebamang A.
Cele, Nkosinathi D.
Mabhida, Sihle E.
Shabalala, Samkelisiwe C.
Penduka, Dambudzo
Opoku, Andy R.
author_sort Mosa, Rebamang A.
collection PubMed
description Control of postprandial hyperglycemia is crucial in the management of diabetes mellitus. Despite the use of the current hypoglycemic drugs, incidence of diabetes and related diseases continue to increase. This study aimed at evaluating the in vivo antihyperglycemic activity of methyl-3β-hydroxylanosta-9,24-dien-21-oate (RA-3), a lanosteryl triterpene isolated, and characterized from Protorhus longifolia stem bark. Spectroscopic data analysis was used to establish and verify the structure of the triterpene. The antihyperglycemic activity of the triterpene was evaluated in an STZ-induced diabetes rat model. The experimental animals were orally administered with RA-3 (100 mg/kg body weight) daily for 14 days. An oral glucose tolerance test was also performed. The animals were euthanized and biochemical analysis of antioxidant status, some glycolytic enzymes and glycogen content were conducted on serum and liver samples, respectively. RA-3 exhibited hypoglycemic activity by reducing blood glucose levels by 37%. The triterpene also improved glucose tolerance in the diabetic rats. Relatively higher hepatic glycogen content, hexokinase and glucokinase activity with a decrease in glucose-6-phosphatase activity were observed in the triterpene-treated diabetic group when compared with the diabetic control group. The triterpene treatment further increased antioxidant status of the diabetic animals; increased activity of superoxide dismutase and catalase were observed along with a decrease in malondialdehyde content. The results indicate potential pharmaceutical effects of lanosteryl triterpene in the management of diabetes mellitus.
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spelling pubmed-63319052019-01-24 In Vivo Antihyperglycemic Activity of a Lanosteryl Triterpene from Protorhus longifolia Mosa, Rebamang A. Cele, Nkosinathi D. Mabhida, Sihle E. Shabalala, Samkelisiwe C. Penduka, Dambudzo Opoku, Andy R. Molecules Article Control of postprandial hyperglycemia is crucial in the management of diabetes mellitus. Despite the use of the current hypoglycemic drugs, incidence of diabetes and related diseases continue to increase. This study aimed at evaluating the in vivo antihyperglycemic activity of methyl-3β-hydroxylanosta-9,24-dien-21-oate (RA-3), a lanosteryl triterpene isolated, and characterized from Protorhus longifolia stem bark. Spectroscopic data analysis was used to establish and verify the structure of the triterpene. The antihyperglycemic activity of the triterpene was evaluated in an STZ-induced diabetes rat model. The experimental animals were orally administered with RA-3 (100 mg/kg body weight) daily for 14 days. An oral glucose tolerance test was also performed. The animals were euthanized and biochemical analysis of antioxidant status, some glycolytic enzymes and glycogen content were conducted on serum and liver samples, respectively. RA-3 exhibited hypoglycemic activity by reducing blood glucose levels by 37%. The triterpene also improved glucose tolerance in the diabetic rats. Relatively higher hepatic glycogen content, hexokinase and glucokinase activity with a decrease in glucose-6-phosphatase activity were observed in the triterpene-treated diabetic group when compared with the diabetic control group. The triterpene treatment further increased antioxidant status of the diabetic animals; increased activity of superoxide dismutase and catalase were observed along with a decrease in malondialdehyde content. The results indicate potential pharmaceutical effects of lanosteryl triterpene in the management of diabetes mellitus. MDPI 2015-07-22 /pmc/articles/PMC6331905/ /pubmed/26205060 http://dx.doi.org/10.3390/molecules200713374 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mosa, Rebamang A.
Cele, Nkosinathi D.
Mabhida, Sihle E.
Shabalala, Samkelisiwe C.
Penduka, Dambudzo
Opoku, Andy R.
In Vivo Antihyperglycemic Activity of a Lanosteryl Triterpene from Protorhus longifolia
title In Vivo Antihyperglycemic Activity of a Lanosteryl Triterpene from Protorhus longifolia
title_full In Vivo Antihyperglycemic Activity of a Lanosteryl Triterpene from Protorhus longifolia
title_fullStr In Vivo Antihyperglycemic Activity of a Lanosteryl Triterpene from Protorhus longifolia
title_full_unstemmed In Vivo Antihyperglycemic Activity of a Lanosteryl Triterpene from Protorhus longifolia
title_short In Vivo Antihyperglycemic Activity of a Lanosteryl Triterpene from Protorhus longifolia
title_sort in vivo antihyperglycemic activity of a lanosteryl triterpene from protorhus longifolia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331905/
https://www.ncbi.nlm.nih.gov/pubmed/26205060
http://dx.doi.org/10.3390/molecules200713374
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