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Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry
Traditional medicine has led to the discovery of important active substances used in several health-related areas. Phytochemicals in Rhoeo discolor extracts have proven to have important antimicrobial activity. In the present study, our group determined the antimicrobial effects of extracts of Rhoeo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331916/ https://www.ncbi.nlm.nih.gov/pubmed/26473824 http://dx.doi.org/10.3390/molecules201018685 |
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author | García-Varela, Rebeca García-García, Rebeca M. Barba-Dávila, Bertha A. Fajardo-Ramírez, Oscar R. Serna-Saldívar, Sergio O. Cardineau, Guy A. |
author_facet | García-Varela, Rebeca García-García, Rebeca M. Barba-Dávila, Bertha A. Fajardo-Ramírez, Oscar R. Serna-Saldívar, Sergio O. Cardineau, Guy A. |
author_sort | García-Varela, Rebeca |
collection | PubMed |
description | Traditional medicine has led to the discovery of important active substances used in several health-related areas. Phytochemicals in Rhoeo discolor extracts have proven to have important antimicrobial activity. In the present study, our group determined the antimicrobial effects of extracts of Rhoeo discolor, a plant commonly used in Mexico for both medicinal and ornamental purposes. We evaluated the in vitro activity of phenolic rich extracts against specifically chosen microorganisms of human health importance by measuring their susceptibility via agar-disc diffusion assay and flow cytometry: Gram-positive Listeria innocua and Streptococcus mutans, Gram-negative Escherichia coli and Pseudomonas aeruginosa, and lastly a fungal pathogen Candida albicans. Ten different extracts were tested in eight different doses on all the microorganisms. Analytical data revealed a high content of phenolic compounds. Both agar-disc diffusion assay and flow cytometry results demonstrated that Pseudomonas aeruginosa was the least affected by extract exposure. However, low doses of these extracts (predominantly polar), in a range from 1 to 4 μg/mL, did produce a statistically significant bacteriostatic and bactericidal effect on the rest of the microorganisms. These results suggest the addition of certain natural extracts from Rhoeo discolor could act as antibacterial and antimycotic drugs or additives for foods and cosmetics. |
format | Online Article Text |
id | pubmed-6331916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63319162019-01-24 Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry García-Varela, Rebeca García-García, Rebeca M. Barba-Dávila, Bertha A. Fajardo-Ramírez, Oscar R. Serna-Saldívar, Sergio O. Cardineau, Guy A. Molecules Article Traditional medicine has led to the discovery of important active substances used in several health-related areas. Phytochemicals in Rhoeo discolor extracts have proven to have important antimicrobial activity. In the present study, our group determined the antimicrobial effects of extracts of Rhoeo discolor, a plant commonly used in Mexico for both medicinal and ornamental purposes. We evaluated the in vitro activity of phenolic rich extracts against specifically chosen microorganisms of human health importance by measuring their susceptibility via agar-disc diffusion assay and flow cytometry: Gram-positive Listeria innocua and Streptococcus mutans, Gram-negative Escherichia coli and Pseudomonas aeruginosa, and lastly a fungal pathogen Candida albicans. Ten different extracts were tested in eight different doses on all the microorganisms. Analytical data revealed a high content of phenolic compounds. Both agar-disc diffusion assay and flow cytometry results demonstrated that Pseudomonas aeruginosa was the least affected by extract exposure. However, low doses of these extracts (predominantly polar), in a range from 1 to 4 μg/mL, did produce a statistically significant bacteriostatic and bactericidal effect on the rest of the microorganisms. These results suggest the addition of certain natural extracts from Rhoeo discolor could act as antibacterial and antimycotic drugs or additives for foods and cosmetics. MDPI 2015-10-14 /pmc/articles/PMC6331916/ /pubmed/26473824 http://dx.doi.org/10.3390/molecules201018685 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article García-Varela, Rebeca García-García, Rebeca M. Barba-Dávila, Bertha A. Fajardo-Ramírez, Oscar R. Serna-Saldívar, Sergio O. Cardineau, Guy A. Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry |
title | Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry |
title_full | Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry |
title_fullStr | Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry |
title_full_unstemmed | Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry |
title_short | Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry |
title_sort | antimicrobial activity of rhoeo discolor phenolic rich extracts determined by flow cytometry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331916/ https://www.ncbi.nlm.nih.gov/pubmed/26473824 http://dx.doi.org/10.3390/molecules201018685 |
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