Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers

The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplifi...

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Autores principales: Mei, Zhiqiang, Zhou, Boxu, Wei, Chunli, Cheng, Jingliang, Imani, Saber, Chen, Hanchun, Fu, Junjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331946/
https://www.ncbi.nlm.nih.gov/pubmed/26569205
http://dx.doi.org/10.3390/molecules201119687
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author Mei, Zhiqiang
Zhou, Boxu
Wei, Chunli
Cheng, Jingliang
Imani, Saber
Chen, Hanchun
Fu, Junjiang
author_facet Mei, Zhiqiang
Zhou, Boxu
Wei, Chunli
Cheng, Jingliang
Imani, Saber
Chen, Hanchun
Fu, Junjiang
author_sort Mei, Zhiqiang
collection PubMed
description The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides.
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spelling pubmed-63319462019-01-24 Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers Mei, Zhiqiang Zhou, Boxu Wei, Chunli Cheng, Jingliang Imani, Saber Chen, Hanchun Fu, Junjiang Molecules Article The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides. MDPI 2015-11-10 /pmc/articles/PMC6331946/ /pubmed/26569205 http://dx.doi.org/10.3390/molecules201119687 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mei, Zhiqiang
Zhou, Boxu
Wei, Chunli
Cheng, Jingliang
Imani, Saber
Chen, Hanchun
Fu, Junjiang
Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers
title Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers
title_full Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers
title_fullStr Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers
title_full_unstemmed Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers
title_short Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers
title_sort genetic authentication of gardenia jasminoides ellis var. grandiflora nakai by improved rapd-derived dna markers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331946/
https://www.ncbi.nlm.nih.gov/pubmed/26569205
http://dx.doi.org/10.3390/molecules201119687
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