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Plasma Pharmacokinetics of Polyphenols in a Traditional Japanese Medicine, Jumihaidokuto, Which Suppresses Propionibacterium acnes-Induced Dermatitis in Rats

Most orally administered polyphenols are metabolized, with very little absorbed as aglycones and/or unchanged forms. Metabolic and pharmacokinetic studies are therefore necessary to understand the pharmacological mechanisms of polyphenols. Jumihaidokuto (JHT), a traditional Japanese medicine, has be...

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Autores principales: Matsumoto, Takashi, Matsubara, Yousuke, Mizuhara, Yasuharu, Sekiguchi, Kyoji, Koseki, Junichi, Tsuchiya, Kazuaki, Nishimura, Hiroaki, Watanabe, Junko, Kaneko, Atsushi, Maemura, Kazuya, Hattori, Tomohisa, Kase, Yoshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332076/
https://www.ncbi.nlm.nih.gov/pubmed/26437394
http://dx.doi.org/10.3390/molecules201018031
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author Matsumoto, Takashi
Matsubara, Yousuke
Mizuhara, Yasuharu
Sekiguchi, Kyoji
Koseki, Junichi
Tsuchiya, Kazuaki
Nishimura, Hiroaki
Watanabe, Junko
Kaneko, Atsushi
Maemura, Kazuya
Hattori, Tomohisa
Kase, Yoshio
author_facet Matsumoto, Takashi
Matsubara, Yousuke
Mizuhara, Yasuharu
Sekiguchi, Kyoji
Koseki, Junichi
Tsuchiya, Kazuaki
Nishimura, Hiroaki
Watanabe, Junko
Kaneko, Atsushi
Maemura, Kazuya
Hattori, Tomohisa
Kase, Yoshio
author_sort Matsumoto, Takashi
collection PubMed
description Most orally administered polyphenols are metabolized, with very little absorbed as aglycones and/or unchanged forms. Metabolic and pharmacokinetic studies are therefore necessary to understand the pharmacological mechanisms of polyphenols. Jumihaidokuto (JHT), a traditional Japanese medicine, has been used for treatment of skin diseases including inflammatory acne. Because JHT contains various types of bioactive polyphenols, our aim was to clarify the metabolism and pharmacokinetics of the polyphenols in JHT and identify active metabolites contributing to its antidermatitis effects. Orally administered JHT inhibited the increase in ear thickness in rats induced by intradermal injection of Propionibacterium acnes. Quantification by LC-MS/MS indicated that JHT contains various types of flavonoids and is also rich in hydrolysable tannins, such as 1,2,3,4,6-penta-O-galloyl glucose. Pharmacokinetic and antioxidant analyses showed that some flavonoid conjugates, such as genistein 7-O-glucuronide and liquiritigenin 7-O-glucuronide, appeared in rat plasma and had an activity to inhibit hydrogen peroxide-dependent oxidation. Furthermore, 4-O-methylgallic acid, a metabolite of Gallic acid, appeared in rat plasma and inhibited the nitric oxide reaction. JHT has numerous polyphenols; it inhibited dermatitis probably via the antioxidant effect of its metabolites. Our study is beneficial for understanding in vivo actions of orally administered polyphenol drugs.
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spelling pubmed-63320762019-01-24 Plasma Pharmacokinetics of Polyphenols in a Traditional Japanese Medicine, Jumihaidokuto, Which Suppresses Propionibacterium acnes-Induced Dermatitis in Rats Matsumoto, Takashi Matsubara, Yousuke Mizuhara, Yasuharu Sekiguchi, Kyoji Koseki, Junichi Tsuchiya, Kazuaki Nishimura, Hiroaki Watanabe, Junko Kaneko, Atsushi Maemura, Kazuya Hattori, Tomohisa Kase, Yoshio Molecules Article Most orally administered polyphenols are metabolized, with very little absorbed as aglycones and/or unchanged forms. Metabolic and pharmacokinetic studies are therefore necessary to understand the pharmacological mechanisms of polyphenols. Jumihaidokuto (JHT), a traditional Japanese medicine, has been used for treatment of skin diseases including inflammatory acne. Because JHT contains various types of bioactive polyphenols, our aim was to clarify the metabolism and pharmacokinetics of the polyphenols in JHT and identify active metabolites contributing to its antidermatitis effects. Orally administered JHT inhibited the increase in ear thickness in rats induced by intradermal injection of Propionibacterium acnes. Quantification by LC-MS/MS indicated that JHT contains various types of flavonoids and is also rich in hydrolysable tannins, such as 1,2,3,4,6-penta-O-galloyl glucose. Pharmacokinetic and antioxidant analyses showed that some flavonoid conjugates, such as genistein 7-O-glucuronide and liquiritigenin 7-O-glucuronide, appeared in rat plasma and had an activity to inhibit hydrogen peroxide-dependent oxidation. Furthermore, 4-O-methylgallic acid, a metabolite of Gallic acid, appeared in rat plasma and inhibited the nitric oxide reaction. JHT has numerous polyphenols; it inhibited dermatitis probably via the antioxidant effect of its metabolites. Our study is beneficial for understanding in vivo actions of orally administered polyphenol drugs. MDPI 2015-09-30 /pmc/articles/PMC6332076/ /pubmed/26437394 http://dx.doi.org/10.3390/molecules201018031 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matsumoto, Takashi
Matsubara, Yousuke
Mizuhara, Yasuharu
Sekiguchi, Kyoji
Koseki, Junichi
Tsuchiya, Kazuaki
Nishimura, Hiroaki
Watanabe, Junko
Kaneko, Atsushi
Maemura, Kazuya
Hattori, Tomohisa
Kase, Yoshio
Plasma Pharmacokinetics of Polyphenols in a Traditional Japanese Medicine, Jumihaidokuto, Which Suppresses Propionibacterium acnes-Induced Dermatitis in Rats
title Plasma Pharmacokinetics of Polyphenols in a Traditional Japanese Medicine, Jumihaidokuto, Which Suppresses Propionibacterium acnes-Induced Dermatitis in Rats
title_full Plasma Pharmacokinetics of Polyphenols in a Traditional Japanese Medicine, Jumihaidokuto, Which Suppresses Propionibacterium acnes-Induced Dermatitis in Rats
title_fullStr Plasma Pharmacokinetics of Polyphenols in a Traditional Japanese Medicine, Jumihaidokuto, Which Suppresses Propionibacterium acnes-Induced Dermatitis in Rats
title_full_unstemmed Plasma Pharmacokinetics of Polyphenols in a Traditional Japanese Medicine, Jumihaidokuto, Which Suppresses Propionibacterium acnes-Induced Dermatitis in Rats
title_short Plasma Pharmacokinetics of Polyphenols in a Traditional Japanese Medicine, Jumihaidokuto, Which Suppresses Propionibacterium acnes-Induced Dermatitis in Rats
title_sort plasma pharmacokinetics of polyphenols in a traditional japanese medicine, jumihaidokuto, which suppresses propionibacterium acnes-induced dermatitis in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332076/
https://www.ncbi.nlm.nih.gov/pubmed/26437394
http://dx.doi.org/10.3390/molecules201018031
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