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Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability

Sedum sarmentosum is an important Chinese medicinal herb that exhibits anti-inflammatory, anti-angiogenic and anti-nociceptive properties. However, little is known about its genetic background. The first set of 14 microsatellite markers were isolated and characterized for S. sarmentosum using an SSR...

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Autores principales: Xu, Jing, Hou, Fu-Yuan, Wan, Ding-Rong, Wang, Sha, Xu, Dong-Mei, Yang, Guang-Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332276/
https://www.ncbi.nlm.nih.gov/pubmed/26556327
http://dx.doi.org/10.3390/molecules201119669
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author Xu, Jing
Hou, Fu-Yuan
Wan, Ding-Rong
Wang, Sha
Xu, Dong-Mei
Yang, Guang-Zhong
author_facet Xu, Jing
Hou, Fu-Yuan
Wan, Ding-Rong
Wang, Sha
Xu, Dong-Mei
Yang, Guang-Zhong
author_sort Xu, Jing
collection PubMed
description Sedum sarmentosum is an important Chinese medicinal herb that exhibits anti-inflammatory, anti-angiogenic and anti-nociceptive properties. However, little is known about its genetic background. The first set of 14 microsatellite markers were isolated and characterized for S. sarmentosum using an SSR-enriched library. Fourteen polymorphic microsatellite markers were acquired with satisfactory amplifications and a polymorphic pattern in 48 S. sarmentosum individuals. The number of alleles ranged from 3 to 15. The observed and expected heterozygosities varied from 0.0833 to 0.8750 and 0.2168 to 0.9063, respectively. Two loci showed significant departure from the Hardy-Weinberg equilibrium. Cross-species amplification was carried out in other Sedum species. High rates of cross-species amplification were observed. The transferability value ranged from 85.7% in S. lineare to 64.3% in S. ellacombianum. These markers will be valuable for studying the genetic variation, population structure and germplasm characterization of S. sarmentosum and related Sedum species.
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spelling pubmed-63322762019-01-24 Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability Xu, Jing Hou, Fu-Yuan Wan, Ding-Rong Wang, Sha Xu, Dong-Mei Yang, Guang-Zhong Molecules Article Sedum sarmentosum is an important Chinese medicinal herb that exhibits anti-inflammatory, anti-angiogenic and anti-nociceptive properties. However, little is known about its genetic background. The first set of 14 microsatellite markers were isolated and characterized for S. sarmentosum using an SSR-enriched library. Fourteen polymorphic microsatellite markers were acquired with satisfactory amplifications and a polymorphic pattern in 48 S. sarmentosum individuals. The number of alleles ranged from 3 to 15. The observed and expected heterozygosities varied from 0.0833 to 0.8750 and 0.2168 to 0.9063, respectively. Two loci showed significant departure from the Hardy-Weinberg equilibrium. Cross-species amplification was carried out in other Sedum species. High rates of cross-species amplification were observed. The transferability value ranged from 85.7% in S. lineare to 64.3% in S. ellacombianum. These markers will be valuable for studying the genetic variation, population structure and germplasm characterization of S. sarmentosum and related Sedum species. MDPI 2015-11-05 /pmc/articles/PMC6332276/ /pubmed/26556327 http://dx.doi.org/10.3390/molecules201119669 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Jing
Hou, Fu-Yuan
Wan, Ding-Rong
Wang, Sha
Xu, Dong-Mei
Yang, Guang-Zhong
Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability
title Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability
title_full Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability
title_fullStr Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability
title_full_unstemmed Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability
title_short Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability
title_sort development and characterization of polymorphic microsatellite markers for sedum sarmentosum (crassulaceae) and their cross-species transferability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332276/
https://www.ncbi.nlm.nih.gov/pubmed/26556327
http://dx.doi.org/10.3390/molecules201119669
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