Cargando…

Methanolic Extract of Ganoderma lucidum Induces Autophagy of AGS Human Gastric Tumor Cells

Ganoderma lucidum is one of the most widely studied mushroom species, particularly in what concerns its medicinal properties. Previous studies (including those from some of us) have shown some evidence that the methanolic extract of G. lucidum affects cellular autophagy. However, it was not known if...

Descripción completa

Detalles Bibliográficos
Autores principales: Reis, Filipa S., Lima, Raquel T., Morales, Patricia, Ferreira, Isabel C. F. R., Vasconcelos, M. Helena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332321/
https://www.ncbi.nlm.nih.gov/pubmed/26426001
http://dx.doi.org/10.3390/molecules201017872
_version_ 1783387323587624960
author Reis, Filipa S.
Lima, Raquel T.
Morales, Patricia
Ferreira, Isabel C. F. R.
Vasconcelos, M. Helena
author_facet Reis, Filipa S.
Lima, Raquel T.
Morales, Patricia
Ferreira, Isabel C. F. R.
Vasconcelos, M. Helena
author_sort Reis, Filipa S.
collection PubMed
description Ganoderma lucidum is one of the most widely studied mushroom species, particularly in what concerns its medicinal properties. Previous studies (including those from some of us) have shown some evidence that the methanolic extract of G. lucidum affects cellular autophagy. However, it was not known if it induces autophagy or decreases the autophagic flux. The treatment of a gastric adenocarcinoma cell line (AGS) with the mushroom extract increased the formation of autophagosomes (vacuoles typical from autophagy). Moreover, the cellular levels of LC3-II were also increased, and the cellular levels of p62 decreased, confirming that the extract affects cellular autophagy. Treating the cells with the extract together with lysossomal protease inhibitors, the cellular levels of LC3-II and p62 increased. The results obtained proved that, in AGS cells, the methanolic extract of G. lucidum causes an induction of autophagy, rather than a reduction in the autophagic flux. To our knowledge, this is the first study proving that statement.
format Online
Article
Text
id pubmed-6332321
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63323212019-01-24 Methanolic Extract of Ganoderma lucidum Induces Autophagy of AGS Human Gastric Tumor Cells Reis, Filipa S. Lima, Raquel T. Morales, Patricia Ferreira, Isabel C. F. R. Vasconcelos, M. Helena Molecules Article Ganoderma lucidum is one of the most widely studied mushroom species, particularly in what concerns its medicinal properties. Previous studies (including those from some of us) have shown some evidence that the methanolic extract of G. lucidum affects cellular autophagy. However, it was not known if it induces autophagy or decreases the autophagic flux. The treatment of a gastric adenocarcinoma cell line (AGS) with the mushroom extract increased the formation of autophagosomes (vacuoles typical from autophagy). Moreover, the cellular levels of LC3-II were also increased, and the cellular levels of p62 decreased, confirming that the extract affects cellular autophagy. Treating the cells with the extract together with lysossomal protease inhibitors, the cellular levels of LC3-II and p62 increased. The results obtained proved that, in AGS cells, the methanolic extract of G. lucidum causes an induction of autophagy, rather than a reduction in the autophagic flux. To our knowledge, this is the first study proving that statement. MDPI 2015-09-29 /pmc/articles/PMC6332321/ /pubmed/26426001 http://dx.doi.org/10.3390/molecules201017872 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reis, Filipa S.
Lima, Raquel T.
Morales, Patricia
Ferreira, Isabel C. F. R.
Vasconcelos, M. Helena
Methanolic Extract of Ganoderma lucidum Induces Autophagy of AGS Human Gastric Tumor Cells
title Methanolic Extract of Ganoderma lucidum Induces Autophagy of AGS Human Gastric Tumor Cells
title_full Methanolic Extract of Ganoderma lucidum Induces Autophagy of AGS Human Gastric Tumor Cells
title_fullStr Methanolic Extract of Ganoderma lucidum Induces Autophagy of AGS Human Gastric Tumor Cells
title_full_unstemmed Methanolic Extract of Ganoderma lucidum Induces Autophagy of AGS Human Gastric Tumor Cells
title_short Methanolic Extract of Ganoderma lucidum Induces Autophagy of AGS Human Gastric Tumor Cells
title_sort methanolic extract of ganoderma lucidum induces autophagy of ags human gastric tumor cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332321/
https://www.ncbi.nlm.nih.gov/pubmed/26426001
http://dx.doi.org/10.3390/molecules201017872
work_keys_str_mv AT reisfilipas methanolicextractofganodermaluciduminducesautophagyofagshumangastrictumorcells
AT limaraquelt methanolicextractofganodermaluciduminducesautophagyofagshumangastrictumorcells
AT moralespatricia methanolicextractofganodermaluciduminducesautophagyofagshumangastrictumorcells
AT ferreiraisabelcfr methanolicextractofganodermaluciduminducesautophagyofagshumangastrictumorcells
AT vasconcelosmhelena methanolicextractofganodermaluciduminducesautophagyofagshumangastrictumorcells