Cargando…
Evaluation of Antimicrobial Efficiency of New Polymers Comprised by Covalently Attached and/or Electrostatically Bound Bacteriostatic Species, Based on Quaternary Ammonium Compounds
In the present work a detailed study of new bacteriostatic copolymers with quaternized ammonium groups introduced in the polymer chain through covalent attachment or electrostatic interaction, was performed. Different copolymers have been considered since beside the active species, the hydrophobic/h...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332343/ https://www.ncbi.nlm.nih.gov/pubmed/26633329 http://dx.doi.org/10.3390/molecules201219768 |
_version_ | 1783387328721453056 |
---|---|
author | Kougia, Efstathia Tselepi, Maria Vasilopoulos, Gavriil Lainioti, Georgia Ch. Koromilas, Nikos D. Druvari, Denisa Bokias, Georgios Vantarakis, Apostolos Kallitsis, Joannis K. |
author_facet | Kougia, Efstathia Tselepi, Maria Vasilopoulos, Gavriil Lainioti, Georgia Ch. Koromilas, Nikos D. Druvari, Denisa Bokias, Georgios Vantarakis, Apostolos Kallitsis, Joannis K. |
author_sort | Kougia, Efstathia |
collection | PubMed |
description | In the present work a detailed study of new bacteriostatic copolymers with quaternized ammonium groups introduced in the polymer chain through covalent attachment or electrostatic interaction, was performed. Different copolymers have been considered since beside the active species, the hydrophobic/hydrophilic nature of the co-monomer was also evaluated in the case of covalently attached bacteriostatic groups, aiming at achieving permanent antibacterial activity. Homopolymers with quaternized ammonium/phosphonium groups were also tested for comparison reasons. The antimicrobial activity of the synthesized polymers after 3 and 24 h of exposure at 4 and 22 °C was investigated on cultures of Gram-negative (P. aeruginosa, E. coli) and Gram-positive (S. aureus, E. faecalis) bacteria. It was found that the combination of the hydrophilic monomer acrylic acid (AA), at low contents, with the covalently attached bacteriostatic group vinyl benzyl dimethylhexadecylammonium chloride (VBCHAM) in the copolymer P(AA-co-VBCHAM88), resulted in a high bacteriostatic activity against P. aeruginosa and E. faecalis (6 log reduction in certain cases). Moreover, the combination of covalently attached VBCHAM units with electrostatically bound cetyltrimethylammonium 4-styrene sulfonate (SSAmC(16)) units in the P(SSAmC(16)-co-VBCHAMx) copolymers led to efficient antimicrobial materials, especially against Gram-positive bacteria, where a log reduction between 4.9 and 6.2 was verified. These materials remain remarkably efficient even when they are incorporated in polysulfone membranes. |
format | Online Article Text |
id | pubmed-6332343 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63323432019-01-24 Evaluation of Antimicrobial Efficiency of New Polymers Comprised by Covalently Attached and/or Electrostatically Bound Bacteriostatic Species, Based on Quaternary Ammonium Compounds Kougia, Efstathia Tselepi, Maria Vasilopoulos, Gavriil Lainioti, Georgia Ch. Koromilas, Nikos D. Druvari, Denisa Bokias, Georgios Vantarakis, Apostolos Kallitsis, Joannis K. Molecules Article In the present work a detailed study of new bacteriostatic copolymers with quaternized ammonium groups introduced in the polymer chain through covalent attachment or electrostatic interaction, was performed. Different copolymers have been considered since beside the active species, the hydrophobic/hydrophilic nature of the co-monomer was also evaluated in the case of covalently attached bacteriostatic groups, aiming at achieving permanent antibacterial activity. Homopolymers with quaternized ammonium/phosphonium groups were also tested for comparison reasons. The antimicrobial activity of the synthesized polymers after 3 and 24 h of exposure at 4 and 22 °C was investigated on cultures of Gram-negative (P. aeruginosa, E. coli) and Gram-positive (S. aureus, E. faecalis) bacteria. It was found that the combination of the hydrophilic monomer acrylic acid (AA), at low contents, with the covalently attached bacteriostatic group vinyl benzyl dimethylhexadecylammonium chloride (VBCHAM) in the copolymer P(AA-co-VBCHAM88), resulted in a high bacteriostatic activity against P. aeruginosa and E. faecalis (6 log reduction in certain cases). Moreover, the combination of covalently attached VBCHAM units with electrostatically bound cetyltrimethylammonium 4-styrene sulfonate (SSAmC(16)) units in the P(SSAmC(16)-co-VBCHAMx) copolymers led to efficient antimicrobial materials, especially against Gram-positive bacteria, where a log reduction between 4.9 and 6.2 was verified. These materials remain remarkably efficient even when they are incorporated in polysulfone membranes. MDPI 2015-12-01 /pmc/articles/PMC6332343/ /pubmed/26633329 http://dx.doi.org/10.3390/molecules201219768 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kougia, Efstathia Tselepi, Maria Vasilopoulos, Gavriil Lainioti, Georgia Ch. Koromilas, Nikos D. Druvari, Denisa Bokias, Georgios Vantarakis, Apostolos Kallitsis, Joannis K. Evaluation of Antimicrobial Efficiency of New Polymers Comprised by Covalently Attached and/or Electrostatically Bound Bacteriostatic Species, Based on Quaternary Ammonium Compounds |
title | Evaluation of Antimicrobial Efficiency of New Polymers Comprised by Covalently Attached and/or Electrostatically Bound Bacteriostatic Species, Based on Quaternary Ammonium Compounds |
title_full | Evaluation of Antimicrobial Efficiency of New Polymers Comprised by Covalently Attached and/or Electrostatically Bound Bacteriostatic Species, Based on Quaternary Ammonium Compounds |
title_fullStr | Evaluation of Antimicrobial Efficiency of New Polymers Comprised by Covalently Attached and/or Electrostatically Bound Bacteriostatic Species, Based on Quaternary Ammonium Compounds |
title_full_unstemmed | Evaluation of Antimicrobial Efficiency of New Polymers Comprised by Covalently Attached and/or Electrostatically Bound Bacteriostatic Species, Based on Quaternary Ammonium Compounds |
title_short | Evaluation of Antimicrobial Efficiency of New Polymers Comprised by Covalently Attached and/or Electrostatically Bound Bacteriostatic Species, Based on Quaternary Ammonium Compounds |
title_sort | evaluation of antimicrobial efficiency of new polymers comprised by covalently attached and/or electrostatically bound bacteriostatic species, based on quaternary ammonium compounds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332343/ https://www.ncbi.nlm.nih.gov/pubmed/26633329 http://dx.doi.org/10.3390/molecules201219768 |
work_keys_str_mv | AT kougiaefstathia evaluationofantimicrobialefficiencyofnewpolymerscomprisedbycovalentlyattachedandorelectrostaticallyboundbacteriostaticspeciesbasedonquaternaryammoniumcompounds AT tselepimaria evaluationofantimicrobialefficiencyofnewpolymerscomprisedbycovalentlyattachedandorelectrostaticallyboundbacteriostaticspeciesbasedonquaternaryammoniumcompounds AT vasilopoulosgavriil evaluationofantimicrobialefficiencyofnewpolymerscomprisedbycovalentlyattachedandorelectrostaticallyboundbacteriostaticspeciesbasedonquaternaryammoniumcompounds AT lainiotigeorgiach evaluationofantimicrobialefficiencyofnewpolymerscomprisedbycovalentlyattachedandorelectrostaticallyboundbacteriostaticspeciesbasedonquaternaryammoniumcompounds AT koromilasnikosd evaluationofantimicrobialefficiencyofnewpolymerscomprisedbycovalentlyattachedandorelectrostaticallyboundbacteriostaticspeciesbasedonquaternaryammoniumcompounds AT druvaridenisa evaluationofantimicrobialefficiencyofnewpolymerscomprisedbycovalentlyattachedandorelectrostaticallyboundbacteriostaticspeciesbasedonquaternaryammoniumcompounds AT bokiasgeorgios evaluationofantimicrobialefficiencyofnewpolymerscomprisedbycovalentlyattachedandorelectrostaticallyboundbacteriostaticspeciesbasedonquaternaryammoniumcompounds AT vantarakisapostolos evaluationofantimicrobialefficiencyofnewpolymerscomprisedbycovalentlyattachedandorelectrostaticallyboundbacteriostaticspeciesbasedonquaternaryammoniumcompounds AT kallitsisjoannisk evaluationofantimicrobialefficiencyofnewpolymerscomprisedbycovalentlyattachedandorelectrostaticallyboundbacteriostaticspeciesbasedonquaternaryammoniumcompounds |