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Exploring the Oxidation of Lignin-Derived Phenols by a Library of Laccase Mutants
Saturation mutagenesis was performed over six residues delimiting the substrate binding pocket of a fungal laccase previously engineered in the lab. Mutant libraries were screened using sinapic acid as a model substrate, and those mutants presenting increased activity were selected for exploring the...
Autores principales: | Pardo, Isabel, Camarero, Susana |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332420/ https://www.ncbi.nlm.nih.gov/pubmed/26364626 http://dx.doi.org/10.3390/molecules200915929 |
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