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Antimycobacterial Activities of Endolysins Derived From a Mycobacteriophage, BTCU-1

The high incidence of Mycobacterium infection, notably multidrug-resistant M. tuberculosis infection, has become a significant public health concern worldwide. In this study, we isolate and analyze a mycobacteriophage, BTCU-1, and a foundational study was performed to evaluate the antimycobacterial...

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Autores principales: Lai, Meng-Jiun, Liu, Chih-Chin, Jiang, Shinn-Jong, Soo, Po-Chi, Tu, Meng-Hsuan, Lee, Jen-Jyh, Chen, Ying-Huei, Chang, Kai-Chih
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332426/
https://www.ncbi.nlm.nih.gov/pubmed/26506338
http://dx.doi.org/10.3390/molecules201019277
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author Lai, Meng-Jiun
Liu, Chih-Chin
Jiang, Shinn-Jong
Soo, Po-Chi
Tu, Meng-Hsuan
Lee, Jen-Jyh
Chen, Ying-Huei
Chang, Kai-Chih
author_facet Lai, Meng-Jiun
Liu, Chih-Chin
Jiang, Shinn-Jong
Soo, Po-Chi
Tu, Meng-Hsuan
Lee, Jen-Jyh
Chen, Ying-Huei
Chang, Kai-Chih
author_sort Lai, Meng-Jiun
collection PubMed
description The high incidence of Mycobacterium infection, notably multidrug-resistant M. tuberculosis infection, has become a significant public health concern worldwide. In this study, we isolate and analyze a mycobacteriophage, BTCU-1, and a foundational study was performed to evaluate the antimycobacterial activity of BTCU-1 and its cloned lytic endolysins. Using Mycobacterium smegmatis as host, a mycobacteriophage, BTCU-1, was isolated from soil in eastern Taiwan. The electron microscopy images revealed that BTCU-1 displayed morphology resembling the Siphoviridae family. In the genome of BTCU-1, two putative lytic genes, BTCU-1_ORF7 and BTCU-1_ORF8 (termed lysA and lysB, respectively), were identified, and further subcloned and expressed in Escherichia coli. When applied exogenously, both LysA and LysB were active against M. smegmatis tested. Scanning electron microscopy revealed that LysA and LysB caused a remarkable modification of the cell shape of M. smegmatis. Intracellular bactericidal activity assay showed that treatment of M. smegmatis—infected RAW 264.7 macrophages with LysA or LysB resulted in a significant reduction in the number of viable intracellular bacilli. These results indicate that the endolysins derived from BTCU-1 have antimycobacterial activity, and suggest that they are good candidates for therapeutic/disinfectant agents to control mycobacterial infections.
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spelling pubmed-63324262019-01-24 Antimycobacterial Activities of Endolysins Derived From a Mycobacteriophage, BTCU-1 Lai, Meng-Jiun Liu, Chih-Chin Jiang, Shinn-Jong Soo, Po-Chi Tu, Meng-Hsuan Lee, Jen-Jyh Chen, Ying-Huei Chang, Kai-Chih Molecules Article The high incidence of Mycobacterium infection, notably multidrug-resistant M. tuberculosis infection, has become a significant public health concern worldwide. In this study, we isolate and analyze a mycobacteriophage, BTCU-1, and a foundational study was performed to evaluate the antimycobacterial activity of BTCU-1 and its cloned lytic endolysins. Using Mycobacterium smegmatis as host, a mycobacteriophage, BTCU-1, was isolated from soil in eastern Taiwan. The electron microscopy images revealed that BTCU-1 displayed morphology resembling the Siphoviridae family. In the genome of BTCU-1, two putative lytic genes, BTCU-1_ORF7 and BTCU-1_ORF8 (termed lysA and lysB, respectively), were identified, and further subcloned and expressed in Escherichia coli. When applied exogenously, both LysA and LysB were active against M. smegmatis tested. Scanning electron microscopy revealed that LysA and LysB caused a remarkable modification of the cell shape of M. smegmatis. Intracellular bactericidal activity assay showed that treatment of M. smegmatis—infected RAW 264.7 macrophages with LysA or LysB resulted in a significant reduction in the number of viable intracellular bacilli. These results indicate that the endolysins derived from BTCU-1 have antimycobacterial activity, and suggest that they are good candidates for therapeutic/disinfectant agents to control mycobacterial infections. MDPI 2015-10-22 /pmc/articles/PMC6332426/ /pubmed/26506338 http://dx.doi.org/10.3390/molecules201019277 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lai, Meng-Jiun
Liu, Chih-Chin
Jiang, Shinn-Jong
Soo, Po-Chi
Tu, Meng-Hsuan
Lee, Jen-Jyh
Chen, Ying-Huei
Chang, Kai-Chih
Antimycobacterial Activities of Endolysins Derived From a Mycobacteriophage, BTCU-1
title Antimycobacterial Activities of Endolysins Derived From a Mycobacteriophage, BTCU-1
title_full Antimycobacterial Activities of Endolysins Derived From a Mycobacteriophage, BTCU-1
title_fullStr Antimycobacterial Activities of Endolysins Derived From a Mycobacteriophage, BTCU-1
title_full_unstemmed Antimycobacterial Activities of Endolysins Derived From a Mycobacteriophage, BTCU-1
title_short Antimycobacterial Activities of Endolysins Derived From a Mycobacteriophage, BTCU-1
title_sort antimycobacterial activities of endolysins derived from a mycobacteriophage, btcu-1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332426/
https://www.ncbi.nlm.nih.gov/pubmed/26506338
http://dx.doi.org/10.3390/molecules201019277
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