Cargando…

A pilot study and case reports on endometrial microbiota and pregnancy outcome: An analysis using 16S rRNA gene sequencing among IVF patients, and trial therapeutic intervention for dysbiotic endometrium

PURPOSE: The present study aimed to analyze the pregnancy outcomes of IVF patients presenting Lactobacillus‐dominated microbiota (LDM) or non‐Lactobacillus‐dominated microbiota (NLDM) of their endometrium and to report cases who were treated for NLDM concurrently with antibiotics and prebiotic/probi...

Descripción completa

Detalles Bibliográficos
Autores principales: Kyono, Koichi, Hashimoto, Tomoko, Kikuchi, Suguru, Nagai, Yoko, Sakuraba, Yoshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332758/
https://www.ncbi.nlm.nih.gov/pubmed/30655724
http://dx.doi.org/10.1002/rmb2.12250
_version_ 1783387424206880768
author Kyono, Koichi
Hashimoto, Tomoko
Kikuchi, Suguru
Nagai, Yoko
Sakuraba, Yoshiyuki
author_facet Kyono, Koichi
Hashimoto, Tomoko
Kikuchi, Suguru
Nagai, Yoko
Sakuraba, Yoshiyuki
author_sort Kyono, Koichi
collection PubMed
description PURPOSE: The present study aimed to analyze the pregnancy outcomes of IVF patients presenting Lactobacillus‐dominated microbiota (LDM) or non‐Lactobacillus‐dominated microbiota (NLDM) of their endometrium and to report cases who were treated for NLDM concurrently with antibiotics and prebiotic/probiotic supplements in a Japanese infertile population. METHODS: Ninety‐two IVF patients were recruited from August 2017 to March 2018. Endometrial fluid samples for sequencing were collected using an IUI catheter. The bacterial status of the endometrium and the pregnancy outcomes were analyzed. For cases with NLDM, antibiotics and prebiotics/probiotics were administered according to their individual microbial conditions. RESULTS: Forty‐seven cases (51.1%) presented LDM and 45 cases (48.9%) presented NLDM at initial analysis. Nine Patients with NLDM were treated by antibiotics and prebiotics/probiotics, and successfully became Lactobacillus‐dominant. Pregnancy rates by single vitrified‐warmed blastocyst transfers were higher in the LDM group (58.9% per patient and 36.3% per FBT) than in the NLDM group (47.2% per patient and 34.7% per FBT) but not significantly different. CONCLUSION: The results of this study could not necessarily prove the clear benefit of establishing Lactobacillus‐dominated endometrium in terms of pregnancy outcome, but there is significance in searching for endometrial microbial status of infertile patients and recovering Lactobacillus‐dominated endometrium might benefit implantation.
format Online
Article
Text
id pubmed-6332758
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-63327582019-01-17 A pilot study and case reports on endometrial microbiota and pregnancy outcome: An analysis using 16S rRNA gene sequencing among IVF patients, and trial therapeutic intervention for dysbiotic endometrium Kyono, Koichi Hashimoto, Tomoko Kikuchi, Suguru Nagai, Yoko Sakuraba, Yoshiyuki Reprod Med Biol Original Articles PURPOSE: The present study aimed to analyze the pregnancy outcomes of IVF patients presenting Lactobacillus‐dominated microbiota (LDM) or non‐Lactobacillus‐dominated microbiota (NLDM) of their endometrium and to report cases who were treated for NLDM concurrently with antibiotics and prebiotic/probiotic supplements in a Japanese infertile population. METHODS: Ninety‐two IVF patients were recruited from August 2017 to March 2018. Endometrial fluid samples for sequencing were collected using an IUI catheter. The bacterial status of the endometrium and the pregnancy outcomes were analyzed. For cases with NLDM, antibiotics and prebiotics/probiotics were administered according to their individual microbial conditions. RESULTS: Forty‐seven cases (51.1%) presented LDM and 45 cases (48.9%) presented NLDM at initial analysis. Nine Patients with NLDM were treated by antibiotics and prebiotics/probiotics, and successfully became Lactobacillus‐dominant. Pregnancy rates by single vitrified‐warmed blastocyst transfers were higher in the LDM group (58.9% per patient and 36.3% per FBT) than in the NLDM group (47.2% per patient and 34.7% per FBT) but not significantly different. CONCLUSION: The results of this study could not necessarily prove the clear benefit of establishing Lactobacillus‐dominated endometrium in terms of pregnancy outcome, but there is significance in searching for endometrial microbial status of infertile patients and recovering Lactobacillus‐dominated endometrium might benefit implantation. John Wiley and Sons Inc. 2018-10-25 /pmc/articles/PMC6332758/ /pubmed/30655724 http://dx.doi.org/10.1002/rmb2.12250 Text en © 2018 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Kyono, Koichi
Hashimoto, Tomoko
Kikuchi, Suguru
Nagai, Yoko
Sakuraba, Yoshiyuki
A pilot study and case reports on endometrial microbiota and pregnancy outcome: An analysis using 16S rRNA gene sequencing among IVF patients, and trial therapeutic intervention for dysbiotic endometrium
title A pilot study and case reports on endometrial microbiota and pregnancy outcome: An analysis using 16S rRNA gene sequencing among IVF patients, and trial therapeutic intervention for dysbiotic endometrium
title_full A pilot study and case reports on endometrial microbiota and pregnancy outcome: An analysis using 16S rRNA gene sequencing among IVF patients, and trial therapeutic intervention for dysbiotic endometrium
title_fullStr A pilot study and case reports on endometrial microbiota and pregnancy outcome: An analysis using 16S rRNA gene sequencing among IVF patients, and trial therapeutic intervention for dysbiotic endometrium
title_full_unstemmed A pilot study and case reports on endometrial microbiota and pregnancy outcome: An analysis using 16S rRNA gene sequencing among IVF patients, and trial therapeutic intervention for dysbiotic endometrium
title_short A pilot study and case reports on endometrial microbiota and pregnancy outcome: An analysis using 16S rRNA gene sequencing among IVF patients, and trial therapeutic intervention for dysbiotic endometrium
title_sort pilot study and case reports on endometrial microbiota and pregnancy outcome: an analysis using 16s rrna gene sequencing among ivf patients, and trial therapeutic intervention for dysbiotic endometrium
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332758/
https://www.ncbi.nlm.nih.gov/pubmed/30655724
http://dx.doi.org/10.1002/rmb2.12250
work_keys_str_mv AT kyonokoichi apilotstudyandcasereportsonendometrialmicrobiotaandpregnancyoutcomeananalysisusing16srrnagenesequencingamongivfpatientsandtrialtherapeuticinterventionfordysbioticendometrium
AT hashimototomoko apilotstudyandcasereportsonendometrialmicrobiotaandpregnancyoutcomeananalysisusing16srrnagenesequencingamongivfpatientsandtrialtherapeuticinterventionfordysbioticendometrium
AT kikuchisuguru apilotstudyandcasereportsonendometrialmicrobiotaandpregnancyoutcomeananalysisusing16srrnagenesequencingamongivfpatientsandtrialtherapeuticinterventionfordysbioticendometrium
AT nagaiyoko apilotstudyandcasereportsonendometrialmicrobiotaandpregnancyoutcomeananalysisusing16srrnagenesequencingamongivfpatientsandtrialtherapeuticinterventionfordysbioticendometrium
AT sakurabayoshiyuki apilotstudyandcasereportsonendometrialmicrobiotaandpregnancyoutcomeananalysisusing16srrnagenesequencingamongivfpatientsandtrialtherapeuticinterventionfordysbioticendometrium
AT kyonokoichi pilotstudyandcasereportsonendometrialmicrobiotaandpregnancyoutcomeananalysisusing16srrnagenesequencingamongivfpatientsandtrialtherapeuticinterventionfordysbioticendometrium
AT hashimototomoko pilotstudyandcasereportsonendometrialmicrobiotaandpregnancyoutcomeananalysisusing16srrnagenesequencingamongivfpatientsandtrialtherapeuticinterventionfordysbioticendometrium
AT kikuchisuguru pilotstudyandcasereportsonendometrialmicrobiotaandpregnancyoutcomeananalysisusing16srrnagenesequencingamongivfpatientsandtrialtherapeuticinterventionfordysbioticendometrium
AT nagaiyoko pilotstudyandcasereportsonendometrialmicrobiotaandpregnancyoutcomeananalysisusing16srrnagenesequencingamongivfpatientsandtrialtherapeuticinterventionfordysbioticendometrium
AT sakurabayoshiyuki pilotstudyandcasereportsonendometrialmicrobiotaandpregnancyoutcomeananalysisusing16srrnagenesequencingamongivfpatientsandtrialtherapeuticinterventionfordysbioticendometrium