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Development of golden hamster embryos effectively produced by injection of sperm heads sonicated in Tris‐HCl buffer with EGTA
PURPOSE: To investigate the effects of sperm treatment medium—TCM199 or EGTA in Tris‐HCl buffer (TBS + EGTA)―for sonication of frozen‐thawed hamster spermatozoa in terms of sperm chromosome integrity and development of hamster oocytes injected with the sperm heads (ICSI). METHODS: Frozen‐thawed hams...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332760/ https://www.ncbi.nlm.nih.gov/pubmed/30655725 http://dx.doi.org/10.1002/rmb2.12253 |