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Comparison of scopolamine-induced cognitive impairment responses in three different ICR stocks
Cognitive impairment responses are important research topics in the study of degenerative brain diseases as well as in understanding of human mental activities. To compare response to scopolamine (SPL)-induced cognitive impairment, we measured altered parameters for learning and memory ability, infl...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Association for Laboratory Animal Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333609/ https://www.ncbi.nlm.nih.gov/pubmed/30671121 http://dx.doi.org/10.5625/lar.2018.34.4.317 |
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author | Yoon, Woo Bin Choi, Hyeon Jun Kim, Ji Eun Park, Ji Won Kang, Mi Ju Bae, Su Ji Lee, Young Ju Choi, You Sang Kim, Kil Soo Jung, Young-Suk Cho, Joon-Yong Hwang, Dae Youn Song, Hyun Keun |
author_facet | Yoon, Woo Bin Choi, Hyeon Jun Kim, Ji Eun Park, Ji Won Kang, Mi Ju Bae, Su Ji Lee, Young Ju Choi, You Sang Kim, Kil Soo Jung, Young-Suk Cho, Joon-Yong Hwang, Dae Youn Song, Hyun Keun |
author_sort | Yoon, Woo Bin |
collection | PubMed |
description | Cognitive impairment responses are important research topics in the study of degenerative brain diseases as well as in understanding of human mental activities. To compare response to scopolamine (SPL)-induced cognitive impairment, we measured altered parameters for learning and memory ability, inflammatory response, oxidative stress, cholinergic dysfunction and neuronal cell damages, in Korl:ICR stock and two commercial breeder stocks (A:ICR and B:ICR) after relevant SPL exposure. In the water maze test, Korl:ICR showed no significant difference in SPL-induced learning and memory impairment compared to the two different ICRs, although escape latency was increased after SPL exposure. Although behavioral assessment using the manual avoidance test revealed reduced latency in all ICR mice after SPL treatment as compared to Vehicle, no differences were observed between the three ICR stocks. To determine cholinergic dysfunction induction by SPL exposure, activity of acetylcholinesterase (AChE) assessed in the three ICR stocks revealed no difference of acetylcholinesterase activity. Furthermore, low levels of superoxide dismutase (SOD) activity and high levels of inflammatory cytokines in SPL-treated group were maintained in all three ICR stocks, although some variations were observed between the SPLtreated groups. Neuronal cell damages induced by SPL showed similar response in all three ICR stocks, as assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, Nissl staining analysis and expression analyses of apoptosis-related proteins. Thus, the results of this study provide strong evidence that Korl:ICR is similar to the other two ICR. Stocks in response to learning and memory capacity. |
format | Online Article Text |
id | pubmed-6333609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Korean Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63336092019-01-22 Comparison of scopolamine-induced cognitive impairment responses in three different ICR stocks Yoon, Woo Bin Choi, Hyeon Jun Kim, Ji Eun Park, Ji Won Kang, Mi Ju Bae, Su Ji Lee, Young Ju Choi, You Sang Kim, Kil Soo Jung, Young-Suk Cho, Joon-Yong Hwang, Dae Youn Song, Hyun Keun Lab Anim Res Original Article Cognitive impairment responses are important research topics in the study of degenerative brain diseases as well as in understanding of human mental activities. To compare response to scopolamine (SPL)-induced cognitive impairment, we measured altered parameters for learning and memory ability, inflammatory response, oxidative stress, cholinergic dysfunction and neuronal cell damages, in Korl:ICR stock and two commercial breeder stocks (A:ICR and B:ICR) after relevant SPL exposure. In the water maze test, Korl:ICR showed no significant difference in SPL-induced learning and memory impairment compared to the two different ICRs, although escape latency was increased after SPL exposure. Although behavioral assessment using the manual avoidance test revealed reduced latency in all ICR mice after SPL treatment as compared to Vehicle, no differences were observed between the three ICR stocks. To determine cholinergic dysfunction induction by SPL exposure, activity of acetylcholinesterase (AChE) assessed in the three ICR stocks revealed no difference of acetylcholinesterase activity. Furthermore, low levels of superoxide dismutase (SOD) activity and high levels of inflammatory cytokines in SPL-treated group were maintained in all three ICR stocks, although some variations were observed between the SPLtreated groups. Neuronal cell damages induced by SPL showed similar response in all three ICR stocks, as assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, Nissl staining analysis and expression analyses of apoptosis-related proteins. Thus, the results of this study provide strong evidence that Korl:ICR is similar to the other two ICR. Stocks in response to learning and memory capacity. Korean Association for Laboratory Animal Science 2018-12 2018-12-31 /pmc/articles/PMC6333609/ /pubmed/30671121 http://dx.doi.org/10.5625/lar.2018.34.4.317 Text en Copyright © 2018 Korean Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Yoon, Woo Bin Choi, Hyeon Jun Kim, Ji Eun Park, Ji Won Kang, Mi Ju Bae, Su Ji Lee, Young Ju Choi, You Sang Kim, Kil Soo Jung, Young-Suk Cho, Joon-Yong Hwang, Dae Youn Song, Hyun Keun Comparison of scopolamine-induced cognitive impairment responses in three different ICR stocks |
title | Comparison of scopolamine-induced cognitive impairment responses in three different ICR stocks |
title_full | Comparison of scopolamine-induced cognitive impairment responses in three different ICR stocks |
title_fullStr | Comparison of scopolamine-induced cognitive impairment responses in three different ICR stocks |
title_full_unstemmed | Comparison of scopolamine-induced cognitive impairment responses in three different ICR stocks |
title_short | Comparison of scopolamine-induced cognitive impairment responses in three different ICR stocks |
title_sort | comparison of scopolamine-induced cognitive impairment responses in three different icr stocks |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333609/ https://www.ncbi.nlm.nih.gov/pubmed/30671121 http://dx.doi.org/10.5625/lar.2018.34.4.317 |
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