Peptidoglycan binding protein (PGBP)-modified magnetic nanobeads for efficient magnetic capturing of Staphylococcus aureus associated with sepsis in blood

Peptidoglycan-binding protein-modified magnetic nanobeads (PGBP-MNBs) were prepared for efficient magnetic capturing of Staphylococcus aureus (S. aureus), which is associated with sepsis, using the binding affinity of PGBP for the peptidoglycan (PG) layer on S. aureus. These PGBP-MNBs can simply cap...

Descripción completa

Detalles Bibliográficos
Autores principales: Lim, Jaewoo, Choi, Jongmin, Guk, Kyeonghye, Son, Seong Uk, Lee, Do Kyung, Yeom, Soo-Jin, Kang, Taejoon, Jung, Juyeon, Lim, Eun-Kyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333782/
https://www.ncbi.nlm.nih.gov/pubmed/30644425
http://dx.doi.org/10.1038/s41598-018-37194-2
_version_ 1783387621049761792
author Lim, Jaewoo
Choi, Jongmin
Guk, Kyeonghye
Son, Seong Uk
Lee, Do Kyung
Yeom, Soo-Jin
Kang, Taejoon
Jung, Juyeon
Lim, Eun-Kyung
author_facet Lim, Jaewoo
Choi, Jongmin
Guk, Kyeonghye
Son, Seong Uk
Lee, Do Kyung
Yeom, Soo-Jin
Kang, Taejoon
Jung, Juyeon
Lim, Eun-Kyung
author_sort Lim, Jaewoo
collection PubMed
description Peptidoglycan-binding protein-modified magnetic nanobeads (PGBP-MNBs) were prepared for efficient magnetic capturing of Staphylococcus aureus (S. aureus), which is associated with sepsis, using the binding affinity of PGBP for the peptidoglycan (PG) layer on S. aureus. These PGBP-MNBs can simply capture S. aureus in plasma within 1 hr or even 15 min. Importantly, they also can capture various types of Gram-positive bacteria, such as Bacillus cereus and methicillin-resistant and methicillin-susceptible S. aureus (MRSA and MSSA). We believe that PGBP-based systems will be used to develop diagnostic systems for Gram-positive bacteria-related diseases.
format Online
Article
Text
id pubmed-6333782
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-63337822019-01-16 Peptidoglycan binding protein (PGBP)-modified magnetic nanobeads for efficient magnetic capturing of Staphylococcus aureus associated with sepsis in blood Lim, Jaewoo Choi, Jongmin Guk, Kyeonghye Son, Seong Uk Lee, Do Kyung Yeom, Soo-Jin Kang, Taejoon Jung, Juyeon Lim, Eun-Kyung Sci Rep Article Peptidoglycan-binding protein-modified magnetic nanobeads (PGBP-MNBs) were prepared for efficient magnetic capturing of Staphylococcus aureus (S. aureus), which is associated with sepsis, using the binding affinity of PGBP for the peptidoglycan (PG) layer on S. aureus. These PGBP-MNBs can simply capture S. aureus in plasma within 1 hr or even 15 min. Importantly, they also can capture various types of Gram-positive bacteria, such as Bacillus cereus and methicillin-resistant and methicillin-susceptible S. aureus (MRSA and MSSA). We believe that PGBP-based systems will be used to develop diagnostic systems for Gram-positive bacteria-related diseases. Nature Publishing Group UK 2019-01-15 /pmc/articles/PMC6333782/ /pubmed/30644425 http://dx.doi.org/10.1038/s41598-018-37194-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lim, Jaewoo
Choi, Jongmin
Guk, Kyeonghye
Son, Seong Uk
Lee, Do Kyung
Yeom, Soo-Jin
Kang, Taejoon
Jung, Juyeon
Lim, Eun-Kyung
Peptidoglycan binding protein (PGBP)-modified magnetic nanobeads for efficient magnetic capturing of Staphylococcus aureus associated with sepsis in blood
title Peptidoglycan binding protein (PGBP)-modified magnetic nanobeads for efficient magnetic capturing of Staphylococcus aureus associated with sepsis in blood
title_full Peptidoglycan binding protein (PGBP)-modified magnetic nanobeads for efficient magnetic capturing of Staphylococcus aureus associated with sepsis in blood
title_fullStr Peptidoglycan binding protein (PGBP)-modified magnetic nanobeads for efficient magnetic capturing of Staphylococcus aureus associated with sepsis in blood
title_full_unstemmed Peptidoglycan binding protein (PGBP)-modified magnetic nanobeads for efficient magnetic capturing of Staphylococcus aureus associated with sepsis in blood
title_short Peptidoglycan binding protein (PGBP)-modified magnetic nanobeads for efficient magnetic capturing of Staphylococcus aureus associated with sepsis in blood
title_sort peptidoglycan binding protein (pgbp)-modified magnetic nanobeads for efficient magnetic capturing of staphylococcus aureus associated with sepsis in blood
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333782/
https://www.ncbi.nlm.nih.gov/pubmed/30644425
http://dx.doi.org/10.1038/s41598-018-37194-2
work_keys_str_mv AT limjaewoo peptidoglycanbindingproteinpgbpmodifiedmagneticnanobeadsforefficientmagneticcapturingofstaphylococcusaureusassociatedwithsepsisinblood
AT choijongmin peptidoglycanbindingproteinpgbpmodifiedmagneticnanobeadsforefficientmagneticcapturingofstaphylococcusaureusassociatedwithsepsisinblood
AT gukkyeonghye peptidoglycanbindingproteinpgbpmodifiedmagneticnanobeadsforefficientmagneticcapturingofstaphylococcusaureusassociatedwithsepsisinblood
AT sonseonguk peptidoglycanbindingproteinpgbpmodifiedmagneticnanobeadsforefficientmagneticcapturingofstaphylococcusaureusassociatedwithsepsisinblood
AT leedokyung peptidoglycanbindingproteinpgbpmodifiedmagneticnanobeadsforefficientmagneticcapturingofstaphylococcusaureusassociatedwithsepsisinblood
AT yeomsoojin peptidoglycanbindingproteinpgbpmodifiedmagneticnanobeadsforefficientmagneticcapturingofstaphylococcusaureusassociatedwithsepsisinblood
AT kangtaejoon peptidoglycanbindingproteinpgbpmodifiedmagneticnanobeadsforefficientmagneticcapturingofstaphylococcusaureusassociatedwithsepsisinblood
AT jungjuyeon peptidoglycanbindingproteinpgbpmodifiedmagneticnanobeadsforefficientmagneticcapturingofstaphylococcusaureusassociatedwithsepsisinblood
AT limeunkyung peptidoglycanbindingproteinpgbpmodifiedmagneticnanobeadsforefficientmagneticcapturingofstaphylococcusaureusassociatedwithsepsisinblood

Ejemplares similares