Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways

Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic,...

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Detalles Bibliográficos
Autores principales: Klein, Hannah L., Bačinskaja, Giedrė, Che, Jun, Cheblal, Anais, Elango, Rajula, Epshtein, Anastasiya, Fitzgerald, Devon M., Gómez-González, Belén, Khan, Sharik R., Kumar, Sandeep, Leland, Bryan A., Marie, Léa, Mei, Qian, Miné-Hattab, Judith, Piotrowska, Alicja, Polleys, Erica J., Putnam, Christopher D., Radchenko, Elina A., Saada, Anissia Ait, Sakofsky, Cynthia J., Shim, Eun Yong, Stracy, Mathew, Xia, Jun, Yan, Zhenxin, Yin, Yi, Aguilera, Andrés, Argueso, Juan Lucas, Freudenreich, Catherine H., Gasser, Susan M., Gordenin, Dmitry A., Haber, James E., Ira, Grzegorz, Jinks-Robertson, Sue, King, Megan C., Kolodner, Richard D., Kuzminov, Andrei, Lambert, Sarah AE, Lee, Sang Eun, Miller, Kyle M., Mirkin, Sergei M., Petes, Thomas D., Rosenberg, Susan M., Rothstein, Rodney, Symington, Lorraine S., Zawadzki, Pawel, Kim, Nayun, Lisby, Michael, Malkova, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shared Science Publishers OG 2019
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334234/
https://www.ncbi.nlm.nih.gov/pubmed/30652105
http://dx.doi.org/10.15698/mic2019.01.664
Descripción
Sumario:Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.

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