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Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic,...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Shared Science Publishers OG
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334234/ https://www.ncbi.nlm.nih.gov/pubmed/30652105 http://dx.doi.org/10.15698/mic2019.01.664 |
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| author | Klein, Hannah L. Bačinskaja, Giedrė Che, Jun Cheblal, Anais Elango, Rajula Epshtein, Anastasiya Fitzgerald, Devon M. Gómez-González, Belén Khan, Sharik R. Kumar, Sandeep Leland, Bryan A. Marie, Léa Mei, Qian Miné-Hattab, Judith Piotrowska, Alicja Polleys, Erica J. Putnam, Christopher D. Radchenko, Elina A. Saada, Anissia Ait Sakofsky, Cynthia J. Shim, Eun Yong Stracy, Mathew Xia, Jun Yan, Zhenxin Yin, Yi Aguilera, Andrés Argueso, Juan Lucas Freudenreich, Catherine H. Gasser, Susan M. Gordenin, Dmitry A. Haber, James E. Ira, Grzegorz Jinks-Robertson, Sue King, Megan C. Kolodner, Richard D. Kuzminov, Andrei Lambert, Sarah AE Lee, Sang Eun Miller, Kyle M. Mirkin, Sergei M. Petes, Thomas D. Rosenberg, Susan M. Rothstein, Rodney Symington, Lorraine S. Zawadzki, Pawel Kim, Nayun Lisby, Michael Malkova, Anna |
| author_facet | Klein, Hannah L. Bačinskaja, Giedrė Che, Jun Cheblal, Anais Elango, Rajula Epshtein, Anastasiya Fitzgerald, Devon M. Gómez-González, Belén Khan, Sharik R. Kumar, Sandeep Leland, Bryan A. Marie, Léa Mei, Qian Miné-Hattab, Judith Piotrowska, Alicja Polleys, Erica J. Putnam, Christopher D. Radchenko, Elina A. Saada, Anissia Ait Sakofsky, Cynthia J. Shim, Eun Yong Stracy, Mathew Xia, Jun Yan, Zhenxin Yin, Yi Aguilera, Andrés Argueso, Juan Lucas Freudenreich, Catherine H. Gasser, Susan M. Gordenin, Dmitry A. Haber, James E. Ira, Grzegorz Jinks-Robertson, Sue King, Megan C. Kolodner, Richard D. Kuzminov, Andrei Lambert, Sarah AE Lee, Sang Eun Miller, Kyle M. Mirkin, Sergei M. Petes, Thomas D. Rosenberg, Susan M. Rothstein, Rodney Symington, Lorraine S. Zawadzki, Pawel Kim, Nayun Lisby, Michael Malkova, Anna |
| author_sort | Klein, Hannah L. |
| collection | PubMed |
| description | Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies. |
| format | Online Article Text |
| id | pubmed-6334234 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| publisher | Shared Science Publishers OG |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-63342342019-01-16 Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways Klein, Hannah L. Bačinskaja, Giedrė Che, Jun Cheblal, Anais Elango, Rajula Epshtein, Anastasiya Fitzgerald, Devon M. Gómez-González, Belén Khan, Sharik R. Kumar, Sandeep Leland, Bryan A. Marie, Léa Mei, Qian Miné-Hattab, Judith Piotrowska, Alicja Polleys, Erica J. Putnam, Christopher D. Radchenko, Elina A. Saada, Anissia Ait Sakofsky, Cynthia J. Shim, Eun Yong Stracy, Mathew Xia, Jun Yan, Zhenxin Yin, Yi Aguilera, Andrés Argueso, Juan Lucas Freudenreich, Catherine H. Gasser, Susan M. Gordenin, Dmitry A. Haber, James E. Ira, Grzegorz Jinks-Robertson, Sue King, Megan C. Kolodner, Richard D. Kuzminov, Andrei Lambert, Sarah AE Lee, Sang Eun Miller, Kyle M. Mirkin, Sergei M. Petes, Thomas D. Rosenberg, Susan M. Rothstein, Rodney Symington, Lorraine S. Zawadzki, Pawel Kim, Nayun Lisby, Michael Malkova, Anna Microb Cell Review Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies. Shared Science Publishers OG 2019-01-07 /pmc/articles/PMC6334234/ /pubmed/30652105 http://dx.doi.org/10.15698/mic2019.01.664 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged. |
| spellingShingle | Review Klein, Hannah L. Bačinskaja, Giedrė Che, Jun Cheblal, Anais Elango, Rajula Epshtein, Anastasiya Fitzgerald, Devon M. Gómez-González, Belén Khan, Sharik R. Kumar, Sandeep Leland, Bryan A. Marie, Léa Mei, Qian Miné-Hattab, Judith Piotrowska, Alicja Polleys, Erica J. Putnam, Christopher D. Radchenko, Elina A. Saada, Anissia Ait Sakofsky, Cynthia J. Shim, Eun Yong Stracy, Mathew Xia, Jun Yan, Zhenxin Yin, Yi Aguilera, Andrés Argueso, Juan Lucas Freudenreich, Catherine H. Gasser, Susan M. Gordenin, Dmitry A. Haber, James E. Ira, Grzegorz Jinks-Robertson, Sue King, Megan C. Kolodner, Richard D. Kuzminov, Andrei Lambert, Sarah AE Lee, Sang Eun Miller, Kyle M. Mirkin, Sergei M. Petes, Thomas D. Rosenberg, Susan M. Rothstein, Rodney Symington, Lorraine S. Zawadzki, Pawel Kim, Nayun Lisby, Michael Malkova, Anna Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways |
| title | Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways |
| title_full | Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways |
| title_fullStr | Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways |
| title_full_unstemmed | Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways |
| title_short | Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways |
| title_sort | guidelines for dna recombination and repair studies: cellular assays of dna repair pathways |
| topic | Review |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334234/ https://www.ncbi.nlm.nih.gov/pubmed/30652105 http://dx.doi.org/10.15698/mic2019.01.664 |
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