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Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring
It is widely known that maternal physical exercise is able to induce beneficial improvements in offspring cognition; however, the effects of paternal exercise have not been explored in detail. The present study was designed to evaluate the impact of paternal physical exercise on memory and learning,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334599/ https://www.ncbi.nlm.nih.gov/pubmed/30539818 http://dx.doi.org/10.4103/1673-5374.245473 |
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author | Spindler, Christiano Segabinazi, Ethiane de Meireles, André Luís Ferreira Piazza, Francele Valente Mega, Filipe dos Santos Salvalaggio, Gabriela Achaval, Matilde Elsner, Viviane Rostirola Marcuzzo, Simone |
author_facet | Spindler, Christiano Segabinazi, Ethiane de Meireles, André Luís Ferreira Piazza, Francele Valente Mega, Filipe dos Santos Salvalaggio, Gabriela Achaval, Matilde Elsner, Viviane Rostirola Marcuzzo, Simone |
author_sort | Spindler, Christiano |
collection | PubMed |
description | It is widely known that maternal physical exercise is able to induce beneficial improvements in offspring cognition; however, the effects of paternal exercise have not been explored in detail. The present study was designed to evaluate the impact of paternal physical exercise on memory and learning, neuroplasticity and DNA methylation levels in the hippocampus of male offspring. Adult male Wistar rats were divided into two groups: sedentary or exercised fathers. The paternal preconception exercise protocol consisted of treadmill running, 20 minutes daily, 5 consecutive days per week for 22 days, while the mothers were not trained. After mating, paternal sperm was collected for global DNA methylation analysis. At postnatal day 53, the offspring were euthanized, and the hippocampus was dissected to measure cell survival by 5-bromo-2′-deoxiuridine and to determine the expression of synaptophysin, reelin, brain-derived neurotrophic factor and global DNA methylation levels. To measure spatial memory and learning changes in offspring, the Morris water maze paradigm was used. There was an improvement in spatial learning, as well as a significant decrease in hippocampal global DNA methylation levels in the offspring from exercised fathers compared with those from sedentary ones; however, no changes were observed in neuroplasticity biomarkers brain-derived neurotrophic factor, reelin and 5-bromo-2′-deoxiuridine. Finally, the global DNA methylation of paternal sperm was not significantly changed by physical exercise. These results suggest a link between paternal preconception physical activity and cognitive benefit, which may be associated with hippocampal epigenetic programming in male offspring. However, the biological mechanisms of this modulation remain unclear. |
format | Online Article Text |
id | pubmed-6334599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63345992019-03-01 Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring Spindler, Christiano Segabinazi, Ethiane de Meireles, André Luís Ferreira Piazza, Francele Valente Mega, Filipe dos Santos Salvalaggio, Gabriela Achaval, Matilde Elsner, Viviane Rostirola Marcuzzo, Simone Neural Regen Res Research Article It is widely known that maternal physical exercise is able to induce beneficial improvements in offspring cognition; however, the effects of paternal exercise have not been explored in detail. The present study was designed to evaluate the impact of paternal physical exercise on memory and learning, neuroplasticity and DNA methylation levels in the hippocampus of male offspring. Adult male Wistar rats were divided into two groups: sedentary or exercised fathers. The paternal preconception exercise protocol consisted of treadmill running, 20 minutes daily, 5 consecutive days per week for 22 days, while the mothers were not trained. After mating, paternal sperm was collected for global DNA methylation analysis. At postnatal day 53, the offspring were euthanized, and the hippocampus was dissected to measure cell survival by 5-bromo-2′-deoxiuridine and to determine the expression of synaptophysin, reelin, brain-derived neurotrophic factor and global DNA methylation levels. To measure spatial memory and learning changes in offspring, the Morris water maze paradigm was used. There was an improvement in spatial learning, as well as a significant decrease in hippocampal global DNA methylation levels in the offspring from exercised fathers compared with those from sedentary ones; however, no changes were observed in neuroplasticity biomarkers brain-derived neurotrophic factor, reelin and 5-bromo-2′-deoxiuridine. Finally, the global DNA methylation of paternal sperm was not significantly changed by physical exercise. These results suggest a link between paternal preconception physical activity and cognitive benefit, which may be associated with hippocampal epigenetic programming in male offspring. However, the biological mechanisms of this modulation remain unclear. Medknow Publications & Media Pvt Ltd 2019-03 /pmc/articles/PMC6334599/ /pubmed/30539818 http://dx.doi.org/10.4103/1673-5374.245473 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Research Article Spindler, Christiano Segabinazi, Ethiane de Meireles, André Luís Ferreira Piazza, Francele Valente Mega, Filipe dos Santos Salvalaggio, Gabriela Achaval, Matilde Elsner, Viviane Rostirola Marcuzzo, Simone Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
title | Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
title_full | Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
title_fullStr | Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
title_full_unstemmed | Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
title_short | Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
title_sort | paternal physical exercise modulates global dna methylation status in the hippocampus of male rat offspring |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334599/ https://www.ncbi.nlm.nih.gov/pubmed/30539818 http://dx.doi.org/10.4103/1673-5374.245473 |
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