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Nuclear singlet multimers (NUSIMERs) with long-lived singlet states

Magnetic resonance (NMR) is a powerful tool in chemical analysis, structure determination and in medical diagnostics. Developing novel biological sensors for this field holds promise to better investigate protein structures or target diseases more efficiently. Herein, we explore nuclear spin singlet...

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Detalles Bibliográficos
Autores principales: Saul, Philip, Mamone, Salvatore, Glöggler, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334717/
https://www.ncbi.nlm.nih.gov/pubmed/30746089
http://dx.doi.org/10.1039/c8sc02831a
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author Saul, Philip
Mamone, Salvatore
Glöggler, Stefan
author_facet Saul, Philip
Mamone, Salvatore
Glöggler, Stefan
author_sort Saul, Philip
collection PubMed
description Magnetic resonance (NMR) is a powerful tool in chemical analysis, structure determination and in medical diagnostics. Developing novel biological sensors for this field holds promise to better investigate protein structures or target diseases more efficiently. Herein, we explore nuclear spin singlet states in dendritic macromolecules as a platform molecule to develop stimuli responsive probes. We have developed a nuclear singlet multimer (NUSIMER) based on a generation 5 poly(amidoamine) dendrimer (PAMAM) which contains on average about 90 accessible nuclear spin singlet states with lifetimes up to 10-fold longer than the T(1) relaxation times (up to 10 seconds T(s)vs. T(1) < 0.5 seconds) in a single molecule. We demonstrate little influence on the singlet lifetime in phosphate buffer (H(2)O) and a high viscosity gel environment in the presence of paramagnetic oxygen. Additionally, we demonstrate an increase in singlet lifetime upon the release of a protective chemical moiety from the NUSIMER following a stimulus, whereby no change in longitudinal relaxation time is observed. The robustness and change in singlet lifetime of the NUSIMER holds promise for the development of a novel type of biosensors.
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spelling pubmed-63347172019-02-11 Nuclear singlet multimers (NUSIMERs) with long-lived singlet states Saul, Philip Mamone, Salvatore Glöggler, Stefan Chem Sci Chemistry Magnetic resonance (NMR) is a powerful tool in chemical analysis, structure determination and in medical diagnostics. Developing novel biological sensors for this field holds promise to better investigate protein structures or target diseases more efficiently. Herein, we explore nuclear spin singlet states in dendritic macromolecules as a platform molecule to develop stimuli responsive probes. We have developed a nuclear singlet multimer (NUSIMER) based on a generation 5 poly(amidoamine) dendrimer (PAMAM) which contains on average about 90 accessible nuclear spin singlet states with lifetimes up to 10-fold longer than the T(1) relaxation times (up to 10 seconds T(s)vs. T(1) < 0.5 seconds) in a single molecule. We demonstrate little influence on the singlet lifetime in phosphate buffer (H(2)O) and a high viscosity gel environment in the presence of paramagnetic oxygen. Additionally, we demonstrate an increase in singlet lifetime upon the release of a protective chemical moiety from the NUSIMER following a stimulus, whereby no change in longitudinal relaxation time is observed. The robustness and change in singlet lifetime of the NUSIMER holds promise for the development of a novel type of biosensors. Royal Society of Chemistry 2018-10-25 /pmc/articles/PMC6334717/ /pubmed/30746089 http://dx.doi.org/10.1039/c8sc02831a Text en This journal is © The Royal Society of Chemistry 2019 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Saul, Philip
Mamone, Salvatore
Glöggler, Stefan
Nuclear singlet multimers (NUSIMERs) with long-lived singlet states
title Nuclear singlet multimers (NUSIMERs) with long-lived singlet states
title_full Nuclear singlet multimers (NUSIMERs) with long-lived singlet states
title_fullStr Nuclear singlet multimers (NUSIMERs) with long-lived singlet states
title_full_unstemmed Nuclear singlet multimers (NUSIMERs) with long-lived singlet states
title_short Nuclear singlet multimers (NUSIMERs) with long-lived singlet states
title_sort nuclear singlet multimers (nusimers) with long-lived singlet states
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334717/
https://www.ncbi.nlm.nih.gov/pubmed/30746089
http://dx.doi.org/10.1039/c8sc02831a
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