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Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing

Heterochromatin Protein 1 (HP1) is a conserved chromosomal protein in eukaryotic cells that has a major role in directing heterochromatin formation, a process that requires co-transcriptional gene silencing mediated by small RNAs and their associated argonaute proteins. Heterochromatin formation req...

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Autores principales: Yang, Fu, Quan, Zhenghui, Huang, Huanwei, He, Minghui, Liu, Xicheng, Cai, Tao, Xi, Rongwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335052/
https://www.ncbi.nlm.nih.gov/pubmed/30648969
http://dx.doi.org/10.7554/eLife.40806
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author Yang, Fu
Quan, Zhenghui
Huang, Huanwei
He, Minghui
Liu, Xicheng
Cai, Tao
Xi, Rongwen
author_facet Yang, Fu
Quan, Zhenghui
Huang, Huanwei
He, Minghui
Liu, Xicheng
Cai, Tao
Xi, Rongwen
author_sort Yang, Fu
collection PubMed
description Heterochromatin Protein 1 (HP1) is a conserved chromosomal protein in eukaryotic cells that has a major role in directing heterochromatin formation, a process that requires co-transcriptional gene silencing mediated by small RNAs and their associated argonaute proteins. Heterochromatin formation requires erasing the active epigenetic mark, such as H3K4me2, but the molecular link between HP1 and H3K4 demethylation remains unclear. In a fertility screen in female Drosophila, we identified ovaries absent (ova), which functions in the stem cell niche, downstream of Piwi, to support germline stem cell differentiation. Moreover, ova acts as a suppressor of position effect variegation, and is required for silencing telomeric transposons in the germline. Biochemically, Ova acts to link the H3K4 demethylase dLsd1 to HP1a for local histone modifications. Therefore, our study provides a molecular connection between HP1a and local H3K4 demethylation during HP1a-mediated gene silencing that is required for ovary development, transposon silencing, and heterochromatin formation. Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter).
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spelling pubmed-63350522019-01-24 Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing Yang, Fu Quan, Zhenghui Huang, Huanwei He, Minghui Liu, Xicheng Cai, Tao Xi, Rongwen eLife Stem Cells and Regenerative Medicine Heterochromatin Protein 1 (HP1) is a conserved chromosomal protein in eukaryotic cells that has a major role in directing heterochromatin formation, a process that requires co-transcriptional gene silencing mediated by small RNAs and their associated argonaute proteins. Heterochromatin formation requires erasing the active epigenetic mark, such as H3K4me2, but the molecular link between HP1 and H3K4 demethylation remains unclear. In a fertility screen in female Drosophila, we identified ovaries absent (ova), which functions in the stem cell niche, downstream of Piwi, to support germline stem cell differentiation. Moreover, ova acts as a suppressor of position effect variegation, and is required for silencing telomeric transposons in the germline. Biochemically, Ova acts to link the H3K4 demethylase dLsd1 to HP1a for local histone modifications. Therefore, our study provides a molecular connection between HP1a and local H3K4 demethylation during HP1a-mediated gene silencing that is required for ovary development, transposon silencing, and heterochromatin formation. Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter). eLife Sciences Publications, Ltd 2019-01-16 /pmc/articles/PMC6335052/ /pubmed/30648969 http://dx.doi.org/10.7554/eLife.40806 Text en © 2019, Yang et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Stem Cells and Regenerative Medicine
Yang, Fu
Quan, Zhenghui
Huang, Huanwei
He, Minghui
Liu, Xicheng
Cai, Tao
Xi, Rongwen
Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
title Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
title_full Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
title_fullStr Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
title_full_unstemmed Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
title_short Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
title_sort ovaries absent links dlsd1 to hp1a for local h3k4 demethylation required for heterochromatic gene silencing
topic Stem Cells and Regenerative Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335052/
https://www.ncbi.nlm.nih.gov/pubmed/30648969
http://dx.doi.org/10.7554/eLife.40806
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