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Expanding the base editing scope in rice by using Cas9 variants
Base editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology‐directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that substant...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335069/ https://www.ncbi.nlm.nih.gov/pubmed/30051586 http://dx.doi.org/10.1111/pbi.12993 |
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author | Hua, Kai Tao, Xiaoping Zhu, Jian‐Kang |
author_facet | Hua, Kai Tao, Xiaoping Zhu, Jian‐Kang |
author_sort | Hua, Kai |
collection | PubMed |
description | Base editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology‐directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that substantially expand the targetable sites in the rice genome. These new base editors can edit endogenous genes in the rice genome with various efficiencies. Moreover, we show that adenine and cytosine base editing can be simultaneously executed in rice. The new base editors described here will be useful in rice functional genomics research and will advance precision molecular breeding in crops. |
format | Online Article Text |
id | pubmed-6335069 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63350692019-01-23 Expanding the base editing scope in rice by using Cas9 variants Hua, Kai Tao, Xiaoping Zhu, Jian‐Kang Plant Biotechnol J Research Articles Base editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology‐directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that substantially expand the targetable sites in the rice genome. These new base editors can edit endogenous genes in the rice genome with various efficiencies. Moreover, we show that adenine and cytosine base editing can be simultaneously executed in rice. The new base editors described here will be useful in rice functional genomics research and will advance precision molecular breeding in crops. John Wiley and Sons Inc. 2018-10-05 2019-02 /pmc/articles/PMC6335069/ /pubmed/30051586 http://dx.doi.org/10.1111/pbi.12993 Text en © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Hua, Kai Tao, Xiaoping Zhu, Jian‐Kang Expanding the base editing scope in rice by using Cas9 variants |
title | Expanding the base editing scope in rice by using Cas9 variants |
title_full | Expanding the base editing scope in rice by using Cas9 variants |
title_fullStr | Expanding the base editing scope in rice by using Cas9 variants |
title_full_unstemmed | Expanding the base editing scope in rice by using Cas9 variants |
title_short | Expanding the base editing scope in rice by using Cas9 variants |
title_sort | expanding the base editing scope in rice by using cas9 variants |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335069/ https://www.ncbi.nlm.nih.gov/pubmed/30051586 http://dx.doi.org/10.1111/pbi.12993 |
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