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Genome sequence and genetic transformation of a widely distributed and cultivated poplar
Populus alba is widely distributed and cultivated in Europe and Asia. This species has been used for diverse studies. In this study, we assembled a de novo genome sequence of P. alba var. pyramidalis (= P. bolleana) and confirmed its high transformation efficiency and short transformation time by ex...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335071/ https://www.ncbi.nlm.nih.gov/pubmed/30044051 http://dx.doi.org/10.1111/pbi.12989 |
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author | Ma, Jianchao Wan, Dongshi Duan, Bingbing Bai, Xiaotao Bai, Qiuxian Chen, Ningning Ma, Tao |
author_facet | Ma, Jianchao Wan, Dongshi Duan, Bingbing Bai, Xiaotao Bai, Qiuxian Chen, Ningning Ma, Tao |
author_sort | Ma, Jianchao |
collection | PubMed |
description | Populus alba is widely distributed and cultivated in Europe and Asia. This species has been used for diverse studies. In this study, we assembled a de novo genome sequence of P. alba var. pyramidalis (= P. bolleana) and confirmed its high transformation efficiency and short transformation time by experiments. Through a process of hybrid genome assembly, a total of 464 M of the genome was assembled. Annotation analyses predicted 37 901 protein‐coding genes. This genome is highly collinear to that of P. trichocarpa, with most genes having orthologs in the two species. We found a marked expansion of gene families related to histone and the hormone auxin but loss of disease resistance genes in P. alba if compared with the closely related P. trichocarpa. The genome sequence presented here represents a valuable resource for further molecular functional analyses of this species as a new tree model, poplar breeding practices and comparative genomic analyses across different poplars. |
format | Online Article Text |
id | pubmed-6335071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63350712019-01-23 Genome sequence and genetic transformation of a widely distributed and cultivated poplar Ma, Jianchao Wan, Dongshi Duan, Bingbing Bai, Xiaotao Bai, Qiuxian Chen, Ningning Ma, Tao Plant Biotechnol J Research Articles Populus alba is widely distributed and cultivated in Europe and Asia. This species has been used for diverse studies. In this study, we assembled a de novo genome sequence of P. alba var. pyramidalis (= P. bolleana) and confirmed its high transformation efficiency and short transformation time by experiments. Through a process of hybrid genome assembly, a total of 464 M of the genome was assembled. Annotation analyses predicted 37 901 protein‐coding genes. This genome is highly collinear to that of P. trichocarpa, with most genes having orthologs in the two species. We found a marked expansion of gene families related to histone and the hormone auxin but loss of disease resistance genes in P. alba if compared with the closely related P. trichocarpa. The genome sequence presented here represents a valuable resource for further molecular functional analyses of this species as a new tree model, poplar breeding practices and comparative genomic analyses across different poplars. John Wiley and Sons Inc. 2018-10-24 2019-02 /pmc/articles/PMC6335071/ /pubmed/30044051 http://dx.doi.org/10.1111/pbi.12989 Text en © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Ma, Jianchao Wan, Dongshi Duan, Bingbing Bai, Xiaotao Bai, Qiuxian Chen, Ningning Ma, Tao Genome sequence and genetic transformation of a widely distributed and cultivated poplar |
title | Genome sequence and genetic transformation of a widely distributed and cultivated poplar |
title_full | Genome sequence and genetic transformation of a widely distributed and cultivated poplar |
title_fullStr | Genome sequence and genetic transformation of a widely distributed and cultivated poplar |
title_full_unstemmed | Genome sequence and genetic transformation of a widely distributed and cultivated poplar |
title_short | Genome sequence and genetic transformation of a widely distributed and cultivated poplar |
title_sort | genome sequence and genetic transformation of a widely distributed and cultivated poplar |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335071/ https://www.ncbi.nlm.nih.gov/pubmed/30044051 http://dx.doi.org/10.1111/pbi.12989 |
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