Cargando…

Genome sequence and genetic transformation of a widely distributed and cultivated poplar

Populus alba is widely distributed and cultivated in Europe and Asia. This species has been used for diverse studies. In this study, we assembled a de novo genome sequence of P. alba var. pyramidalis (= P. bolleana) and confirmed its high transformation efficiency and short transformation time by ex...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Jianchao, Wan, Dongshi, Duan, Bingbing, Bai, Xiaotao, Bai, Qiuxian, Chen, Ningning, Ma, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335071/
https://www.ncbi.nlm.nih.gov/pubmed/30044051
http://dx.doi.org/10.1111/pbi.12989
_version_ 1783387833374867456
author Ma, Jianchao
Wan, Dongshi
Duan, Bingbing
Bai, Xiaotao
Bai, Qiuxian
Chen, Ningning
Ma, Tao
author_facet Ma, Jianchao
Wan, Dongshi
Duan, Bingbing
Bai, Xiaotao
Bai, Qiuxian
Chen, Ningning
Ma, Tao
author_sort Ma, Jianchao
collection PubMed
description Populus alba is widely distributed and cultivated in Europe and Asia. This species has been used for diverse studies. In this study, we assembled a de novo genome sequence of P. alba var. pyramidalis (= P. bolleana) and confirmed its high transformation efficiency and short transformation time by experiments. Through a process of hybrid genome assembly, a total of 464 M of the genome was assembled. Annotation analyses predicted 37 901 protein‐coding genes. This genome is highly collinear to that of P. trichocarpa, with most genes having orthologs in the two species. We found a marked expansion of gene families related to histone and the hormone auxin but loss of disease resistance genes in P. alba if compared with the closely related P. trichocarpa. The genome sequence presented here represents a valuable resource for further molecular functional analyses of this species as a new tree model, poplar breeding practices and comparative genomic analyses across different poplars.
format Online
Article
Text
id pubmed-6335071
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-63350712019-01-23 Genome sequence and genetic transformation of a widely distributed and cultivated poplar Ma, Jianchao Wan, Dongshi Duan, Bingbing Bai, Xiaotao Bai, Qiuxian Chen, Ningning Ma, Tao Plant Biotechnol J Research Articles Populus alba is widely distributed and cultivated in Europe and Asia. This species has been used for diverse studies. In this study, we assembled a de novo genome sequence of P. alba var. pyramidalis (= P. bolleana) and confirmed its high transformation efficiency and short transformation time by experiments. Through a process of hybrid genome assembly, a total of 464 M of the genome was assembled. Annotation analyses predicted 37 901 protein‐coding genes. This genome is highly collinear to that of P. trichocarpa, with most genes having orthologs in the two species. We found a marked expansion of gene families related to histone and the hormone auxin but loss of disease resistance genes in P. alba if compared with the closely related P. trichocarpa. The genome sequence presented here represents a valuable resource for further molecular functional analyses of this species as a new tree model, poplar breeding practices and comparative genomic analyses across different poplars. John Wiley and Sons Inc. 2018-10-24 2019-02 /pmc/articles/PMC6335071/ /pubmed/30044051 http://dx.doi.org/10.1111/pbi.12989 Text en © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Ma, Jianchao
Wan, Dongshi
Duan, Bingbing
Bai, Xiaotao
Bai, Qiuxian
Chen, Ningning
Ma, Tao
Genome sequence and genetic transformation of a widely distributed and cultivated poplar
title Genome sequence and genetic transformation of a widely distributed and cultivated poplar
title_full Genome sequence and genetic transformation of a widely distributed and cultivated poplar
title_fullStr Genome sequence and genetic transformation of a widely distributed and cultivated poplar
title_full_unstemmed Genome sequence and genetic transformation of a widely distributed and cultivated poplar
title_short Genome sequence and genetic transformation of a widely distributed and cultivated poplar
title_sort genome sequence and genetic transformation of a widely distributed and cultivated poplar
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335071/
https://www.ncbi.nlm.nih.gov/pubmed/30044051
http://dx.doi.org/10.1111/pbi.12989
work_keys_str_mv AT majianchao genomesequenceandgenetictransformationofawidelydistributedandcultivatedpoplar
AT wandongshi genomesequenceandgenetictransformationofawidelydistributedandcultivatedpoplar
AT duanbingbing genomesequenceandgenetictransformationofawidelydistributedandcultivatedpoplar
AT baixiaotao genomesequenceandgenetictransformationofawidelydistributedandcultivatedpoplar
AT baiqiuxian genomesequenceandgenetictransformationofawidelydistributedandcultivatedpoplar
AT chenningning genomesequenceandgenetictransformationofawidelydistributedandcultivatedpoplar
AT matao genomesequenceandgenetictransformationofawidelydistributedandcultivatedpoplar