Cargando…
Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation
Controlled ovarian hyperstimulation (COH) impairs the synchronized development of endometrium and embryo, resulting in the failure of embryo implantation. Here, we investigated what effects electroacupuncture had on embryo implantation in COH rats. Female rats were randomly assigned to four groups:...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335210/ https://www.ncbi.nlm.nih.gov/pubmed/30084973 http://dx.doi.org/10.1093/biolre/ioy176 |
_version_ | 1783387845376868352 |
---|---|
author | Chen, Wei Chen, Jie Xu, Menghao Zhong, Zhiyan Zhang, Qing Yang, Wei Huang, Guangying |
author_facet | Chen, Wei Chen, Jie Xu, Menghao Zhong, Zhiyan Zhang, Qing Yang, Wei Huang, Guangying |
author_sort | Chen, Wei |
collection | PubMed |
description | Controlled ovarian hyperstimulation (COH) impairs the synchronized development of endometrium and embryo, resulting in the failure of embryo implantation. Here, we investigated what effects electroacupuncture had on embryo implantation in COH rats. Female rats were randomly assigned to four groups: normal (N), model (M), electroacupuncture (EA), and electroacupuncture pretreatment (PEA). Rats in groups M, EA, PEA were injected with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin to establish the COH model. Rats in group EA received electroacupuncture treatment from the PMSG injection day to the 3rd day of pregnancy (D3), while those in group PEA received electroacupuncture treatment for 3 days before the PMSG day and continuing to D3. Furthermore, another 30 female rats who received the same treatment as the rats in group PEA were injected with siVEGFR2 into uterine lumen. The endometrial microvascular density (MVD) and the expression levels of vascular endothelial growth factor-A, angiopoietin-1, and fibroblast growth factor-2 were significantly lower in groups M than in groups N and PEA. The percentage of dolichos biflorus agglutinin positive uterine natural killer cells in groups N, EA and PEA was higher than that in group M. After the siVEGFR2 injection, the protein expression levels of vascular endothelial growth factor receptor 2 (VEGFR2), PI3K, p-AKT and p-ERK, the embryo number and the MVD were significantly reduced. In conclusion, electroacupuncture can facilitate embryo implantation in COH rats by activating the VEGFR2/PI3K/AKT and VEGFR2/ERK signaling pathways which have a positive relationship with endometrial angiogenesis. |
format | Online Article Text |
id | pubmed-6335210 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-63352102019-01-24 Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation Chen, Wei Chen, Jie Xu, Menghao Zhong, Zhiyan Zhang, Qing Yang, Wei Huang, Guangying Biol Reprod Research Article Controlled ovarian hyperstimulation (COH) impairs the synchronized development of endometrium and embryo, resulting in the failure of embryo implantation. Here, we investigated what effects electroacupuncture had on embryo implantation in COH rats. Female rats were randomly assigned to four groups: normal (N), model (M), electroacupuncture (EA), and electroacupuncture pretreatment (PEA). Rats in groups M, EA, PEA were injected with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin to establish the COH model. Rats in group EA received electroacupuncture treatment from the PMSG injection day to the 3rd day of pregnancy (D3), while those in group PEA received electroacupuncture treatment for 3 days before the PMSG day and continuing to D3. Furthermore, another 30 female rats who received the same treatment as the rats in group PEA were injected with siVEGFR2 into uterine lumen. The endometrial microvascular density (MVD) and the expression levels of vascular endothelial growth factor-A, angiopoietin-1, and fibroblast growth factor-2 were significantly lower in groups M than in groups N and PEA. The percentage of dolichos biflorus agglutinin positive uterine natural killer cells in groups N, EA and PEA was higher than that in group M. After the siVEGFR2 injection, the protein expression levels of vascular endothelial growth factor receptor 2 (VEGFR2), PI3K, p-AKT and p-ERK, the embryo number and the MVD were significantly reduced. In conclusion, electroacupuncture can facilitate embryo implantation in COH rats by activating the VEGFR2/PI3K/AKT and VEGFR2/ERK signaling pathways which have a positive relationship with endometrial angiogenesis. Oxford University Press 2019-01 2018-08-01 /pmc/articles/PMC6335210/ /pubmed/30084973 http://dx.doi.org/10.1093/biolre/ioy176 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. http://creativecommons.org/licenses/by-nc/4.0/ Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Chen, Wei Chen, Jie Xu, Menghao Zhong, Zhiyan Zhang, Qing Yang, Wei Huang, Guangying Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation |
title | Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation |
title_full | Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation |
title_fullStr | Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation |
title_full_unstemmed | Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation |
title_short | Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation |
title_sort | electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335210/ https://www.ncbi.nlm.nih.gov/pubmed/30084973 http://dx.doi.org/10.1093/biolre/ioy176 |
work_keys_str_mv | AT chenwei electroacupuncturefacilitatesimplantationbyenhancingendometrialangiogenesisinaratmodelofovarianhyperstimulation AT chenjie electroacupuncturefacilitatesimplantationbyenhancingendometrialangiogenesisinaratmodelofovarianhyperstimulation AT xumenghao electroacupuncturefacilitatesimplantationbyenhancingendometrialangiogenesisinaratmodelofovarianhyperstimulation AT zhongzhiyan electroacupuncturefacilitatesimplantationbyenhancingendometrialangiogenesisinaratmodelofovarianhyperstimulation AT zhangqing electroacupuncturefacilitatesimplantationbyenhancingendometrialangiogenesisinaratmodelofovarianhyperstimulation AT yangwei electroacupuncturefacilitatesimplantationbyenhancingendometrialangiogenesisinaratmodelofovarianhyperstimulation AT huangguangying electroacupuncturefacilitatesimplantationbyenhancingendometrialangiogenesisinaratmodelofovarianhyperstimulation |