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Detection of cancer antigens (CA-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor
Integrating microfluidics with biosensors is of great research interest with the increasing trend of lab-on-the chip and point-of-care devices. Though there have been numerous studies performed relating microfluidics to the biosensing mechanisms, the study of the sensitivity variation due to microfl...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335232/ https://www.ncbi.nlm.nih.gov/pubmed/30652204 http://dx.doi.org/10.1186/s40580-019-0173-6 |
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author | Nunna, Bharath Babu Mandal, Debdyuti Lee, Joo Un Singh, Harsimranjit Zhuang, Shiqiang Misra, Durgamadhab Bhuyian, Md Nasir Uddin Lee, Eon Soo |
author_facet | Nunna, Bharath Babu Mandal, Debdyuti Lee, Joo Un Singh, Harsimranjit Zhuang, Shiqiang Misra, Durgamadhab Bhuyian, Md Nasir Uddin Lee, Eon Soo |
author_sort | Nunna, Bharath Babu |
collection | PubMed |
description | Integrating microfluidics with biosensors is of great research interest with the increasing trend of lab-on-the chip and point-of-care devices. Though there have been numerous studies performed relating microfluidics to the biosensing mechanisms, the study of the sensitivity variation due to microfluidic flow is very much limited. In this paper, the sensitivity of interdigitated electrodes was evaluated at the static drop condition and the microfluidic flow condition. In addition, this study demonstrates the use of gold nanoparticles to enhance the sensor signal response and provides experimental results of the capacitance difference during cancer antigen-125 (CA-125) antigen–antibody conjugation at multiple concentrations of CA-125 antigens. The experimental results also provide evidence of disease-specific detection of CA-125 antigen at multiple concentrations with the increase in capacitive signal response proportional to the concentration of the CA-125 antigens. The capacitive signal response of antigen–antibody conjugation on interdigitate electrodes has been enhanced by approximately 2.8 times (from 260.80 to 736.33 pF at 20 kHz frequency) in static drop condition and approximately 2.5 times (from 205.85 to 518.48 pF at 20 kHz frequency) in microfluidic flow condition with gold nanoparticle-coating. The capacitive signal response is observed to decrease at microfluidic flow condition at both plain interdigitated electrodes (from 260.80 to 205.85 pF at 20 kHz frequency) and gold nano particle coated interdigitated electrodes (from 736.33 to 518.48 pF at 20 kHz frequency), due to the strong shear effect compared to static drop condition. However, the microfluidic channel in the biosensor has the potential to increase the signal to noise ratio due to plasma separation from the whole blood and lead to the increase concentration of the biomarkers in the blood volume for sensing. |
format | Online Article Text |
id | pubmed-6335232 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-63352322019-02-01 Detection of cancer antigens (CA-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor Nunna, Bharath Babu Mandal, Debdyuti Lee, Joo Un Singh, Harsimranjit Zhuang, Shiqiang Misra, Durgamadhab Bhuyian, Md Nasir Uddin Lee, Eon Soo Nano Converg Research Integrating microfluidics with biosensors is of great research interest with the increasing trend of lab-on-the chip and point-of-care devices. Though there have been numerous studies performed relating microfluidics to the biosensing mechanisms, the study of the sensitivity variation due to microfluidic flow is very much limited. In this paper, the sensitivity of interdigitated electrodes was evaluated at the static drop condition and the microfluidic flow condition. In addition, this study demonstrates the use of gold nanoparticles to enhance the sensor signal response and provides experimental results of the capacitance difference during cancer antigen-125 (CA-125) antigen–antibody conjugation at multiple concentrations of CA-125 antigens. The experimental results also provide evidence of disease-specific detection of CA-125 antigen at multiple concentrations with the increase in capacitive signal response proportional to the concentration of the CA-125 antigens. The capacitive signal response of antigen–antibody conjugation on interdigitate electrodes has been enhanced by approximately 2.8 times (from 260.80 to 736.33 pF at 20 kHz frequency) in static drop condition and approximately 2.5 times (from 205.85 to 518.48 pF at 20 kHz frequency) in microfluidic flow condition with gold nanoparticle-coating. The capacitive signal response is observed to decrease at microfluidic flow condition at both plain interdigitated electrodes (from 260.80 to 205.85 pF at 20 kHz frequency) and gold nano particle coated interdigitated electrodes (from 736.33 to 518.48 pF at 20 kHz frequency), due to the strong shear effect compared to static drop condition. However, the microfluidic channel in the biosensor has the potential to increase the signal to noise ratio due to plasma separation from the whole blood and lead to the increase concentration of the biomarkers in the blood volume for sensing. Springer Singapore 2019-01-17 /pmc/articles/PMC6335232/ /pubmed/30652204 http://dx.doi.org/10.1186/s40580-019-0173-6 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Nunna, Bharath Babu Mandal, Debdyuti Lee, Joo Un Singh, Harsimranjit Zhuang, Shiqiang Misra, Durgamadhab Bhuyian, Md Nasir Uddin Lee, Eon Soo Detection of cancer antigens (CA-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor |
title | Detection of cancer antigens (CA-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor |
title_full | Detection of cancer antigens (CA-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor |
title_fullStr | Detection of cancer antigens (CA-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor |
title_full_unstemmed | Detection of cancer antigens (CA-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor |
title_short | Detection of cancer antigens (CA-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor |
title_sort | detection of cancer antigens (ca-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335232/ https://www.ncbi.nlm.nih.gov/pubmed/30652204 http://dx.doi.org/10.1186/s40580-019-0173-6 |
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