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Avoidance response to CO(2) in the lateral horn

In flies, the olfactory information is carried from the first relay in the brain, the antennal lobe, to the mushroom body (MB) and the lateral horn (LH). Olfactory associations are formed in the MB. The LH was ascribed a role in innate responses based on the stereotyped connectivity with the antenna...

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Autores principales: Varela, Nélia, Gaspar, Miguel, Dias, Sophie, Vasconcelos, Maria Luísa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336243/
https://www.ncbi.nlm.nih.gov/pubmed/30653496
http://dx.doi.org/10.1371/journal.pbio.2006749
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author Varela, Nélia
Gaspar, Miguel
Dias, Sophie
Vasconcelos, Maria Luísa
author_facet Varela, Nélia
Gaspar, Miguel
Dias, Sophie
Vasconcelos, Maria Luísa
author_sort Varela, Nélia
collection PubMed
description In flies, the olfactory information is carried from the first relay in the brain, the antennal lobe, to the mushroom body (MB) and the lateral horn (LH). Olfactory associations are formed in the MB. The LH was ascribed a role in innate responses based on the stereotyped connectivity with the antennal lobe, stereotyped physiological responses to odors, and MB silencing experiments. Direct evidence for the functional role of the LH is still missing. Here, we investigate the behavioral role of the LH neurons (LHNs) directly, using the CO(2) response as a paradigm. Our results show the involvement of the LH in innate responses. Specifically, we demonstrate that activity in two sets of neurons is required for the full behavioral response to CO(2). Tests of the behavioral response to other odors indicate the neurons are selective to CO(2) response. Using calcium imaging, we observe that the two sets of neurons respond to CO(2) in a different manner. Using independent manipulation and recording of the two sets of neurons, we find that the one that projects to the superior intermediate protocerebrum (SIP) also outputs to the local neurons within the LH. The design of simultaneous output at the LH and the SIP, an output of the MB, allows for coordination between innate and learned responses.
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spelling pubmed-63362432019-01-31 Avoidance response to CO(2) in the lateral horn Varela, Nélia Gaspar, Miguel Dias, Sophie Vasconcelos, Maria Luísa PLoS Biol Short Reports In flies, the olfactory information is carried from the first relay in the brain, the antennal lobe, to the mushroom body (MB) and the lateral horn (LH). Olfactory associations are formed in the MB. The LH was ascribed a role in innate responses based on the stereotyped connectivity with the antennal lobe, stereotyped physiological responses to odors, and MB silencing experiments. Direct evidence for the functional role of the LH is still missing. Here, we investigate the behavioral role of the LH neurons (LHNs) directly, using the CO(2) response as a paradigm. Our results show the involvement of the LH in innate responses. Specifically, we demonstrate that activity in two sets of neurons is required for the full behavioral response to CO(2). Tests of the behavioral response to other odors indicate the neurons are selective to CO(2) response. Using calcium imaging, we observe that the two sets of neurons respond to CO(2) in a different manner. Using independent manipulation and recording of the two sets of neurons, we find that the one that projects to the superior intermediate protocerebrum (SIP) also outputs to the local neurons within the LH. The design of simultaneous output at the LH and the SIP, an output of the MB, allows for coordination between innate and learned responses. Public Library of Science 2019-01-17 /pmc/articles/PMC6336243/ /pubmed/30653496 http://dx.doi.org/10.1371/journal.pbio.2006749 Text en © 2019 Varela et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Short Reports
Varela, Nélia
Gaspar, Miguel
Dias, Sophie
Vasconcelos, Maria Luísa
Avoidance response to CO(2) in the lateral horn
title Avoidance response to CO(2) in the lateral horn
title_full Avoidance response to CO(2) in the lateral horn
title_fullStr Avoidance response to CO(2) in the lateral horn
title_full_unstemmed Avoidance response to CO(2) in the lateral horn
title_short Avoidance response to CO(2) in the lateral horn
title_sort avoidance response to co(2) in the lateral horn
topic Short Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336243/
https://www.ncbi.nlm.nih.gov/pubmed/30653496
http://dx.doi.org/10.1371/journal.pbio.2006749
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