Cargando…

Fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats

Experimental acute lung injury models are often used to increase our knowledge on the acute respiratory distress syndrome (ARDS), however, existing animal models often do not take into account the impact of specific fluid strategies on the development of lung injury. In contrast, the current literat...

Descripción completa

Detalles Bibliográficos
Autores principales: Ingelse, Sarah A., Juschten, Jenny, Maas, Martinus A. W., Matute-Bello, Gustavo, Juffermans, Nicole P., van Woensel, Job B. M., Bem, Reinout A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336323/
https://www.ncbi.nlm.nih.gov/pubmed/30653512
http://dx.doi.org/10.1371/journal.pone.0210172
_version_ 1783388039459897344
author Ingelse, Sarah A.
Juschten, Jenny
Maas, Martinus A. W.
Matute-Bello, Gustavo
Juffermans, Nicole P.
van Woensel, Job B. M.
Bem, Reinout A.
author_facet Ingelse, Sarah A.
Juschten, Jenny
Maas, Martinus A. W.
Matute-Bello, Gustavo
Juffermans, Nicole P.
van Woensel, Job B. M.
Bem, Reinout A.
author_sort Ingelse, Sarah A.
collection PubMed
description Experimental acute lung injury models are often used to increase our knowledge on the acute respiratory distress syndrome (ARDS), however, existing animal models often do not take into account the impact of specific fluid strategies on the development of lung injury. In contrast, the current literature strongly suggests that fluid management strategies have a significant impact on clinical outcome of patients with ARDS. Thus, it is important to characterize the role of fluid management strategies in experimental models of lung injury. In this study we investigated the effect of two different fluid strategies on commonly used outcome variables in a short-term model of acute lung injury, in relation to age. Infant (2–3 weeks) and adult (3–4 months) Wistar rats received intratracheal instillations of lipopolysaccharide and 24 hours later were mechanically ventilated for 6 hours. During mechanical ventilation, rats from both age groups were randomized to either a standard or conservative intravenous fluid strategy. We found that the hemodynamic response in infant and adult rats was similar in both fluid strategies. Lung wet-to-dry ratios were lower in adult, but not in infant rats receiving the conservative fluid strategy as compared to the standard fluid strategy. There were age-related differences in markers of alveolar capillary barrier disruption and alveolar fluid clearance, yet these were unaffected by fluid strategy. Finally, we found significantly higher IL-1β and TNF-α concentrations in the adult rats treated with the conservative as compared to the standard fluid regimen. In conclusion, the choice of fluid strategy in mechanically ventilated rats with experimental LPS-induced acute lung injury has a significant effect on pulmonary extravascular water, an important and well-recognized lung injury marker, and on the local pro-inflammatory cytokine profiles. We advocate the use of a more uniform, conservative, fluid strategy regimen in experimental models of acute lung injury.
format Online
Article
Text
id pubmed-6336323
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-63363232019-01-30 Fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats Ingelse, Sarah A. Juschten, Jenny Maas, Martinus A. W. Matute-Bello, Gustavo Juffermans, Nicole P. van Woensel, Job B. M. Bem, Reinout A. PLoS One Research Article Experimental acute lung injury models are often used to increase our knowledge on the acute respiratory distress syndrome (ARDS), however, existing animal models often do not take into account the impact of specific fluid strategies on the development of lung injury. In contrast, the current literature strongly suggests that fluid management strategies have a significant impact on clinical outcome of patients with ARDS. Thus, it is important to characterize the role of fluid management strategies in experimental models of lung injury. In this study we investigated the effect of two different fluid strategies on commonly used outcome variables in a short-term model of acute lung injury, in relation to age. Infant (2–3 weeks) and adult (3–4 months) Wistar rats received intratracheal instillations of lipopolysaccharide and 24 hours later were mechanically ventilated for 6 hours. During mechanical ventilation, rats from both age groups were randomized to either a standard or conservative intravenous fluid strategy. We found that the hemodynamic response in infant and adult rats was similar in both fluid strategies. Lung wet-to-dry ratios were lower in adult, but not in infant rats receiving the conservative fluid strategy as compared to the standard fluid strategy. There were age-related differences in markers of alveolar capillary barrier disruption and alveolar fluid clearance, yet these were unaffected by fluid strategy. Finally, we found significantly higher IL-1β and TNF-α concentrations in the adult rats treated with the conservative as compared to the standard fluid regimen. In conclusion, the choice of fluid strategy in mechanically ventilated rats with experimental LPS-induced acute lung injury has a significant effect on pulmonary extravascular water, an important and well-recognized lung injury marker, and on the local pro-inflammatory cytokine profiles. We advocate the use of a more uniform, conservative, fluid strategy regimen in experimental models of acute lung injury. Public Library of Science 2019-01-17 /pmc/articles/PMC6336323/ /pubmed/30653512 http://dx.doi.org/10.1371/journal.pone.0210172 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Ingelse, Sarah A.
Juschten, Jenny
Maas, Martinus A. W.
Matute-Bello, Gustavo
Juffermans, Nicole P.
van Woensel, Job B. M.
Bem, Reinout A.
Fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats
title Fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats
title_full Fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats
title_fullStr Fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats
title_full_unstemmed Fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats
title_short Fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats
title_sort fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336323/
https://www.ncbi.nlm.nih.gov/pubmed/30653512
http://dx.doi.org/10.1371/journal.pone.0210172
work_keys_str_mv AT ingelsesaraha fluidrestrictionreducespulmonaryedemainamodelofacutelunginjuryinmechanicallyventilatedrats
AT juschtenjenny fluidrestrictionreducespulmonaryedemainamodelofacutelunginjuryinmechanicallyventilatedrats
AT maasmartinusaw fluidrestrictionreducespulmonaryedemainamodelofacutelunginjuryinmechanicallyventilatedrats
AT matutebellogustavo fluidrestrictionreducespulmonaryedemainamodelofacutelunginjuryinmechanicallyventilatedrats
AT juffermansnicolep fluidrestrictionreducespulmonaryedemainamodelofacutelunginjuryinmechanicallyventilatedrats
AT vanwoenseljobbm fluidrestrictionreducespulmonaryedemainamodelofacutelunginjuryinmechanicallyventilatedrats
AT bemreinouta fluidrestrictionreducespulmonaryedemainamodelofacutelunginjuryinmechanicallyventilatedrats