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A cation diffusion facilitator, GmCDF1, negatively regulates salt tolerance in soybean

Salt stress is one of the major abiotic factors that affect the metabolism, growth and development of plants, and soybean [Glycine max (L.) Merr.] germination is sensitive to salt stress. Thus, to ensure the successful establishment and productivity of soybeans in saline soil, the genetic mechanisms...

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Autores principales: Zhang, Wei, Liao, Xiliang, Cui, Yanmei, Ma, Weiyu, Zhang, Xinnan, Du, Hongyang, Ma, Yujie, Ning, Lihua, Wang, Hui, Huang, Fang, Yang, Hui, Kan, Guizhen, Yu, Deyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336350/
https://www.ncbi.nlm.nih.gov/pubmed/30615606
http://dx.doi.org/10.1371/journal.pgen.1007798
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author Zhang, Wei
Liao, Xiliang
Cui, Yanmei
Ma, Weiyu
Zhang, Xinnan
Du, Hongyang
Ma, Yujie
Ning, Lihua
Wang, Hui
Huang, Fang
Yang, Hui
Kan, Guizhen
Yu, Deyue
author_facet Zhang, Wei
Liao, Xiliang
Cui, Yanmei
Ma, Weiyu
Zhang, Xinnan
Du, Hongyang
Ma, Yujie
Ning, Lihua
Wang, Hui
Huang, Fang
Yang, Hui
Kan, Guizhen
Yu, Deyue
author_sort Zhang, Wei
collection PubMed
description Salt stress is one of the major abiotic factors that affect the metabolism, growth and development of plants, and soybean [Glycine max (L.) Merr.] germination is sensitive to salt stress. Thus, to ensure the successful establishment and productivity of soybeans in saline soil, the genetic mechanisms of salt tolerance at the soybean germination stage need to be explored. In this study, a population of 184 recombinant inbred lines (RILs) was utilized to map quantitative trait loci (QTLs) related to salt tolerance. A major QTL related to salt tolerance at the soybean germination stage named qST-8 was closely linked with the marker Sat_162 and detected on chromosome 8. Interestingly, a genome-wide association study (GWAS) identified several single nucleotide polymorphisms (SNPs) significantly associated with salt tolerance in the same genetic region on chromosome 8. Resequencing, bioinformatics and gene expression analyses were implemented to identify the candidate gene Glyma.08g102000, which belongs to the cation diffusion facilitator (CDF) family and was named GmCDF1. Overexpression and RNA interference of GmCDF1 in soybean hairy roots resulted in increased sensitivity and tolerance to salt stress, respectively. This report provides the first demonstration that GmCDF1 negatively regulates salt tolerance by maintaining K(+)-Na(+) homeostasis in soybean. In addition, GmCDF1 affected the expression of two ion homeostasis-associated genes, salt overly sensitive 1 (GmSOS1) and Na(+)/H(+) exchanger 1 (GmNHX1), in transgenic hairy roots. Moreover, a haplotype analysis detected ten haplotypes of GmCDF1 in 31 soybean genotypes. A candidate-gene association analysis showed that two SNPs in GmCDF1 were significantly associated with salt tolerance and that Hap1 was more sensitive to salt stress than Hap2. The results demonstrated that the expression level of GmCDF1 was negatively correlated with salt tolerance in the 31 soybean accessions (r = -0.56, P < 0.01). Taken together, these results not only indicate that GmCDF1 plays a negative role in soybean salt tolerance but also help elucidate the molecular mechanisms of salt tolerance and accelerate the breeding of salt-tolerant soybean.
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spelling pubmed-63363502019-01-30 A cation diffusion facilitator, GmCDF1, negatively regulates salt tolerance in soybean Zhang, Wei Liao, Xiliang Cui, Yanmei Ma, Weiyu Zhang, Xinnan Du, Hongyang Ma, Yujie Ning, Lihua Wang, Hui Huang, Fang Yang, Hui Kan, Guizhen Yu, Deyue PLoS Genet Research Article Salt stress is one of the major abiotic factors that affect the metabolism, growth and development of plants, and soybean [Glycine max (L.) Merr.] germination is sensitive to salt stress. Thus, to ensure the successful establishment and productivity of soybeans in saline soil, the genetic mechanisms of salt tolerance at the soybean germination stage need to be explored. In this study, a population of 184 recombinant inbred lines (RILs) was utilized to map quantitative trait loci (QTLs) related to salt tolerance. A major QTL related to salt tolerance at the soybean germination stage named qST-8 was closely linked with the marker Sat_162 and detected on chromosome 8. Interestingly, a genome-wide association study (GWAS) identified several single nucleotide polymorphisms (SNPs) significantly associated with salt tolerance in the same genetic region on chromosome 8. Resequencing, bioinformatics and gene expression analyses were implemented to identify the candidate gene Glyma.08g102000, which belongs to the cation diffusion facilitator (CDF) family and was named GmCDF1. Overexpression and RNA interference of GmCDF1 in soybean hairy roots resulted in increased sensitivity and tolerance to salt stress, respectively. This report provides the first demonstration that GmCDF1 negatively regulates salt tolerance by maintaining K(+)-Na(+) homeostasis in soybean. In addition, GmCDF1 affected the expression of two ion homeostasis-associated genes, salt overly sensitive 1 (GmSOS1) and Na(+)/H(+) exchanger 1 (GmNHX1), in transgenic hairy roots. Moreover, a haplotype analysis detected ten haplotypes of GmCDF1 in 31 soybean genotypes. A candidate-gene association analysis showed that two SNPs in GmCDF1 were significantly associated with salt tolerance and that Hap1 was more sensitive to salt stress than Hap2. The results demonstrated that the expression level of GmCDF1 was negatively correlated with salt tolerance in the 31 soybean accessions (r = -0.56, P < 0.01). Taken together, these results not only indicate that GmCDF1 plays a negative role in soybean salt tolerance but also help elucidate the molecular mechanisms of salt tolerance and accelerate the breeding of salt-tolerant soybean. Public Library of Science 2019-01-07 /pmc/articles/PMC6336350/ /pubmed/30615606 http://dx.doi.org/10.1371/journal.pgen.1007798 Text en © 2019 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhang, Wei
Liao, Xiliang
Cui, Yanmei
Ma, Weiyu
Zhang, Xinnan
Du, Hongyang
Ma, Yujie
Ning, Lihua
Wang, Hui
Huang, Fang
Yang, Hui
Kan, Guizhen
Yu, Deyue
A cation diffusion facilitator, GmCDF1, negatively regulates salt tolerance in soybean
title A cation diffusion facilitator, GmCDF1, negatively regulates salt tolerance in soybean
title_full A cation diffusion facilitator, GmCDF1, negatively regulates salt tolerance in soybean
title_fullStr A cation diffusion facilitator, GmCDF1, negatively regulates salt tolerance in soybean
title_full_unstemmed A cation diffusion facilitator, GmCDF1, negatively regulates salt tolerance in soybean
title_short A cation diffusion facilitator, GmCDF1, negatively regulates salt tolerance in soybean
title_sort cation diffusion facilitator, gmcdf1, negatively regulates salt tolerance in soybean
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336350/
https://www.ncbi.nlm.nih.gov/pubmed/30615606
http://dx.doi.org/10.1371/journal.pgen.1007798
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