Royal jelly peptides: potential inhibitors of β-secretase in N2a/APP695swe cells

Royal jelly (RJ) is a type of natural health product with a long history of use. Royal jelly peptides (RJPs) obtained from RJ have numerous bioactivities. To study the neuroprotective effect of RJPs, major royal jelly proteins were digested into crude RJPs and subsequently purified by RP-HPLC. Purif...

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Autores principales: Zhang, Xueqing, Yu, Yi, Sun, Ping, Fan, Zhen, Zhang, Wensheng, Feng, Chengqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336779/
https://www.ncbi.nlm.nih.gov/pubmed/30655564
http://dx.doi.org/10.1038/s41598-018-35801-w
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author Zhang, Xueqing
Yu, Yi
Sun, Ping
Fan, Zhen
Zhang, Wensheng
Feng, Chengqiang
author_facet Zhang, Xueqing
Yu, Yi
Sun, Ping
Fan, Zhen
Zhang, Wensheng
Feng, Chengqiang
author_sort Zhang, Xueqing
collection PubMed
description Royal jelly (RJ) is a type of natural health product with a long history of use. Royal jelly peptides (RJPs) obtained from RJ have numerous bioactivities. To study the neuroprotective effect of RJPs, major royal jelly proteins were digested into crude RJPs and subsequently purified by RP-HPLC. Purified RJP fractions were evaluated in N2a/APP695swe cells. Our results indicated that purified royal jelly peptides (RJPs) (1–9 μg/mL) could inhibit external beta-amyloid 40 (Aβ(1-40)) and beta-amyloid 42 (Aβ(1-42)) production through the down-regulation of β-secretase (BACE1) in N2a/APP695 cells. The modulation of BACE1 may be related to histone acetylation modification. Our results demonstrated a neuroprotective function of RJPs, which indicates that RJPs may serve as potential β-secretase inhibitors in ameliorating Aβ-related pathology in Alzheimer’s Disease.
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spelling pubmed-63367792019-01-22 Royal jelly peptides: potential inhibitors of β-secretase in N2a/APP695swe cells Zhang, Xueqing Yu, Yi Sun, Ping Fan, Zhen Zhang, Wensheng Feng, Chengqiang Sci Rep Article Royal jelly (RJ) is a type of natural health product with a long history of use. Royal jelly peptides (RJPs) obtained from RJ have numerous bioactivities. To study the neuroprotective effect of RJPs, major royal jelly proteins were digested into crude RJPs and subsequently purified by RP-HPLC. Purified RJP fractions were evaluated in N2a/APP695swe cells. Our results indicated that purified royal jelly peptides (RJPs) (1–9 μg/mL) could inhibit external beta-amyloid 40 (Aβ(1-40)) and beta-amyloid 42 (Aβ(1-42)) production through the down-regulation of β-secretase (BACE1) in N2a/APP695 cells. The modulation of BACE1 may be related to histone acetylation modification. Our results demonstrated a neuroprotective function of RJPs, which indicates that RJPs may serve as potential β-secretase inhibitors in ameliorating Aβ-related pathology in Alzheimer’s Disease. Nature Publishing Group UK 2019-01-17 /pmc/articles/PMC6336779/ /pubmed/30655564 http://dx.doi.org/10.1038/s41598-018-35801-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Xueqing
Yu, Yi
Sun, Ping
Fan, Zhen
Zhang, Wensheng
Feng, Chengqiang
Royal jelly peptides: potential inhibitors of β-secretase in N2a/APP695swe cells
title Royal jelly peptides: potential inhibitors of β-secretase in N2a/APP695swe cells
title_full Royal jelly peptides: potential inhibitors of β-secretase in N2a/APP695swe cells
title_fullStr Royal jelly peptides: potential inhibitors of β-secretase in N2a/APP695swe cells
title_full_unstemmed Royal jelly peptides: potential inhibitors of β-secretase in N2a/APP695swe cells
title_short Royal jelly peptides: potential inhibitors of β-secretase in N2a/APP695swe cells
title_sort royal jelly peptides: potential inhibitors of β-secretase in n2a/app695swe cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336779/
https://www.ncbi.nlm.nih.gov/pubmed/30655564
http://dx.doi.org/10.1038/s41598-018-35801-w
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