A novel emulsion PCR method coupled with fluorescence spectrophotometry for simultaneous qualitative, quantitative and high-throughput detection of multiple DNA targets

We constructed and validated a novel emulsion PCR method combined with fluorescence spectrophotometry (EPFS) for simultaneous qualitative, quantitative and high-throughput detection of multiple DNA targets. In a single reaction set, each pair of primers was labeled with a specific fluorophore. Throu...

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Detalles Bibliográficos
Autores principales: Du, Yanan, Zhao, Xiao, Zhao, Binan, Xu, Yan, Shi, Wei, Ren, Fangfang, Wu, Yangyang, Hu, Ruili, Fan, Xiaorui, Zhang, Qi, Zhang, Xiaoxia, Zhang, Wanjing, Wu, Wenjing, Shi, Bin, Zhao, Huanzhen, Zhao, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336815/
https://www.ncbi.nlm.nih.gov/pubmed/30655629
http://dx.doi.org/10.1038/s41598-018-36981-1

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