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A minimal biochemical route towards de novo formation of synthetic phospholipid membranes

All living cells consist of membrane compartments, which are mainly composed of phospholipids. Phospholipid synthesis is catalyzed by membrane-bound enzymes, which themselves require pre-existing membranes for function. Thus, the principle of membrane continuity creates a paradox when considering ho...

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Autores principales: Bhattacharya, Ahanjit, Brea, Roberto J., Niederholtmeyer, Henrike, Devaraj, Neal K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336818/
https://www.ncbi.nlm.nih.gov/pubmed/30655537
http://dx.doi.org/10.1038/s41467-018-08174-x
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author Bhattacharya, Ahanjit
Brea, Roberto J.
Niederholtmeyer, Henrike
Devaraj, Neal K.
author_facet Bhattacharya, Ahanjit
Brea, Roberto J.
Niederholtmeyer, Henrike
Devaraj, Neal K.
author_sort Bhattacharya, Ahanjit
collection PubMed
description All living cells consist of membrane compartments, which are mainly composed of phospholipids. Phospholipid synthesis is catalyzed by membrane-bound enzymes, which themselves require pre-existing membranes for function. Thus, the principle of membrane continuity creates a paradox when considering how the first biochemical membrane-synthesis machinery arose and has hampered efforts to develop simplified pathways for membrane generation in synthetic cells. Here, we develop a high-yielding strategy for de novo formation and growth of phospholipid membranes by repurposing a soluble enzyme FadD10 to form fatty acyl adenylates that react with amine-functionalized lysolipids to form phospholipids. Continuous supply of fresh precursors needed for lipid synthesis enables the growth of vesicles encapsulating FadD10. Using a minimal transcription/translation system, phospholipid vesicles are generated de novo in the presence of DNA encoding FadD10. Our findings suggest that alternate chemistries can produce and maintain synthetic phospholipid membranes and provides a strategy for generating membrane-based materials.
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spelling pubmed-63368182019-01-22 A minimal biochemical route towards de novo formation of synthetic phospholipid membranes Bhattacharya, Ahanjit Brea, Roberto J. Niederholtmeyer, Henrike Devaraj, Neal K. Nat Commun Article All living cells consist of membrane compartments, which are mainly composed of phospholipids. Phospholipid synthesis is catalyzed by membrane-bound enzymes, which themselves require pre-existing membranes for function. Thus, the principle of membrane continuity creates a paradox when considering how the first biochemical membrane-synthesis machinery arose and has hampered efforts to develop simplified pathways for membrane generation in synthetic cells. Here, we develop a high-yielding strategy for de novo formation and growth of phospholipid membranes by repurposing a soluble enzyme FadD10 to form fatty acyl adenylates that react with amine-functionalized lysolipids to form phospholipids. Continuous supply of fresh precursors needed for lipid synthesis enables the growth of vesicles encapsulating FadD10. Using a minimal transcription/translation system, phospholipid vesicles are generated de novo in the presence of DNA encoding FadD10. Our findings suggest that alternate chemistries can produce and maintain synthetic phospholipid membranes and provides a strategy for generating membrane-based materials. Nature Publishing Group UK 2019-01-17 /pmc/articles/PMC6336818/ /pubmed/30655537 http://dx.doi.org/10.1038/s41467-018-08174-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bhattacharya, Ahanjit
Brea, Roberto J.
Niederholtmeyer, Henrike
Devaraj, Neal K.
A minimal biochemical route towards de novo formation of synthetic phospholipid membranes
title A minimal biochemical route towards de novo formation of synthetic phospholipid membranes
title_full A minimal biochemical route towards de novo formation of synthetic phospholipid membranes
title_fullStr A minimal biochemical route towards de novo formation of synthetic phospholipid membranes
title_full_unstemmed A minimal biochemical route towards de novo formation of synthetic phospholipid membranes
title_short A minimal biochemical route towards de novo formation of synthetic phospholipid membranes
title_sort minimal biochemical route towards de novo formation of synthetic phospholipid membranes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336818/
https://www.ncbi.nlm.nih.gov/pubmed/30655537
http://dx.doi.org/10.1038/s41467-018-08174-x
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