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Vibrational Approach to the Dynamics and Structure of Protein Amyloids

Amyloid diseases, including neurodegenerative diseases such as Alzheimer’s and Parkinson’s, are linked to a poorly understood progression of protein misfolding and aggregation events that culminate in tissue-selective deposition and human pathology. Elucidation of the mechanistic details of protein...

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Autores principales: Li, Haoqian, Lantz, Richard, Du, Deguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337179/
https://www.ncbi.nlm.nih.gov/pubmed/30621325
http://dx.doi.org/10.3390/molecules24010186
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author Li, Haoqian
Lantz, Richard
Du, Deguo
author_facet Li, Haoqian
Lantz, Richard
Du, Deguo
author_sort Li, Haoqian
collection PubMed
description Amyloid diseases, including neurodegenerative diseases such as Alzheimer’s and Parkinson’s, are linked to a poorly understood progression of protein misfolding and aggregation events that culminate in tissue-selective deposition and human pathology. Elucidation of the mechanistic details of protein aggregation and the structural features of the aggregates is critical for a comprehensive understanding of the mechanisms of protein oligomerization and fibrillization. Vibrational spectroscopies, such as Fourier transform infrared (FTIR) and Raman, are powerful tools that are sensitive to the secondary structure of proteins and have been widely used to investigate protein misfolding and aggregation. We address the application of the vibrational approaches in recent studies of conformational dynamics and structural characteristics of protein oligomers and amyloid fibrils. In particular, introduction of isotope labelled carbonyl into a peptide backbone, and incorporation of the extrinsic unnatural amino acids with vibrational moieties on the side chain, have greatly expanded the ability of vibrational spectroscopy to obtain site-specific structural and dynamic information. The applications of these methods in recent studies of protein aggregation are also reviewed.
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spelling pubmed-63371792019-01-25 Vibrational Approach to the Dynamics and Structure of Protein Amyloids Li, Haoqian Lantz, Richard Du, Deguo Molecules Review Amyloid diseases, including neurodegenerative diseases such as Alzheimer’s and Parkinson’s, are linked to a poorly understood progression of protein misfolding and aggregation events that culminate in tissue-selective deposition and human pathology. Elucidation of the mechanistic details of protein aggregation and the structural features of the aggregates is critical for a comprehensive understanding of the mechanisms of protein oligomerization and fibrillization. Vibrational spectroscopies, such as Fourier transform infrared (FTIR) and Raman, are powerful tools that are sensitive to the secondary structure of proteins and have been widely used to investigate protein misfolding and aggregation. We address the application of the vibrational approaches in recent studies of conformational dynamics and structural characteristics of protein oligomers and amyloid fibrils. In particular, introduction of isotope labelled carbonyl into a peptide backbone, and incorporation of the extrinsic unnatural amino acids with vibrational moieties on the side chain, have greatly expanded the ability of vibrational spectroscopy to obtain site-specific structural and dynamic information. The applications of these methods in recent studies of protein aggregation are also reviewed. MDPI 2019-01-06 /pmc/articles/PMC6337179/ /pubmed/30621325 http://dx.doi.org/10.3390/molecules24010186 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Li, Haoqian
Lantz, Richard
Du, Deguo
Vibrational Approach to the Dynamics and Structure of Protein Amyloids
title Vibrational Approach to the Dynamics and Structure of Protein Amyloids
title_full Vibrational Approach to the Dynamics and Structure of Protein Amyloids
title_fullStr Vibrational Approach to the Dynamics and Structure of Protein Amyloids
title_full_unstemmed Vibrational Approach to the Dynamics and Structure of Protein Amyloids
title_short Vibrational Approach to the Dynamics and Structure of Protein Amyloids
title_sort vibrational approach to the dynamics and structure of protein amyloids
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337179/
https://www.ncbi.nlm.nih.gov/pubmed/30621325
http://dx.doi.org/10.3390/molecules24010186
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