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Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39

Chitosanases play an important role in chitosan degradation, forming enzymatic degradation products with several biological activities. Although many chitosanases have been discovered and studied, the enzymes with special characteristics are still rather rare. In this study, a new chitosanase, CsnM,...

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Detalles Bibliográficos
Autores principales: Zhou, Yu, Chen, Xuehong, Li, Xiao, Han, Yantao, Wang, Yanan, Yao, Ruyong, Li, Shangyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337222/
https://www.ncbi.nlm.nih.gov/pubmed/30621320
http://dx.doi.org/10.3390/molecules24010183
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author Zhou, Yu
Chen, Xuehong
Li, Xiao
Han, Yantao
Wang, Yanan
Yao, Ruyong
Li, Shangyong
author_facet Zhou, Yu
Chen, Xuehong
Li, Xiao
Han, Yantao
Wang, Yanan
Yao, Ruyong
Li, Shangyong
author_sort Zhou, Yu
collection PubMed
description Chitosanases play an important role in chitosan degradation, forming enzymatic degradation products with several biological activities. Although many chitosanases have been discovered and studied, the enzymes with special characteristics are still rather rare. In this study, a new chitosanase, CsnM, with an apparent molecular weight of 28 kDa was purified from the marine bacterium Pseudoalteromonas sp. SY39. CsnM is a cold-adapted enzyme, which shows highest activity at 40 °C and exhibits 30.6% and 49.4% of its maximal activity at 10 and 15 °C, respectively. CsnM is also a thermo-tolerant enzyme that recovers 95.2%, 89.1% and 88.1% of its initial activity after boiling for 5, 10 and 20 min, respectively. Additionally, CsnM is an endo-type chitosanase that yields chitodisaccharide as the main product (69.9% of the total product). It’s cold-adaptation, thermo-tolerance and high chitodisaccharide yield make CsnM a superior candidate for biotechnological application to produce chitooligosaccharides.
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spelling pubmed-63372222019-01-25 Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39 Zhou, Yu Chen, Xuehong Li, Xiao Han, Yantao Wang, Yanan Yao, Ruyong Li, Shangyong Molecules Article Chitosanases play an important role in chitosan degradation, forming enzymatic degradation products with several biological activities. Although many chitosanases have been discovered and studied, the enzymes with special characteristics are still rather rare. In this study, a new chitosanase, CsnM, with an apparent molecular weight of 28 kDa was purified from the marine bacterium Pseudoalteromonas sp. SY39. CsnM is a cold-adapted enzyme, which shows highest activity at 40 °C and exhibits 30.6% and 49.4% of its maximal activity at 10 and 15 °C, respectively. CsnM is also a thermo-tolerant enzyme that recovers 95.2%, 89.1% and 88.1% of its initial activity after boiling for 5, 10 and 20 min, respectively. Additionally, CsnM is an endo-type chitosanase that yields chitodisaccharide as the main product (69.9% of the total product). It’s cold-adaptation, thermo-tolerance and high chitodisaccharide yield make CsnM a superior candidate for biotechnological application to produce chitooligosaccharides. MDPI 2019-01-06 /pmc/articles/PMC6337222/ /pubmed/30621320 http://dx.doi.org/10.3390/molecules24010183 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Yu
Chen, Xuehong
Li, Xiao
Han, Yantao
Wang, Yanan
Yao, Ruyong
Li, Shangyong
Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39
title Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39
title_full Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39
title_fullStr Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39
title_full_unstemmed Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39
title_short Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39
title_sort purification and characterization of a new cold-adapted and thermo-tolerant chitosanase from marine bacterium pseudoalteromonas sp. sy39
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337222/
https://www.ncbi.nlm.nih.gov/pubmed/30621320
http://dx.doi.org/10.3390/molecules24010183
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