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AXIN1 in Plasma or Serum Is a Potential New Biomarker for Endometriosis

Although endometriosis is considered an inflammatory disease, no reliable diagnostic biomarkers exist for use in clinical practice. The aim was to investigate the inflammatory profile in endometriosis using an exploratory approach of inflammation-related proteins. Patients with laparoscopy-verified...

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Autores principales: Ek, Malin, Roth, Bodil, Engström, Gunnar, Ohlsson, Bodil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337238/
https://www.ncbi.nlm.nih.gov/pubmed/30621017
http://dx.doi.org/10.3390/ijms20010189
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author Ek, Malin
Roth, Bodil
Engström, Gunnar
Ohlsson, Bodil
author_facet Ek, Malin
Roth, Bodil
Engström, Gunnar
Ohlsson, Bodil
author_sort Ek, Malin
collection PubMed
description Although endometriosis is considered an inflammatory disease, no reliable diagnostic biomarkers exist for use in clinical practice. The aim was to investigate the inflammatory profile in endometriosis using an exploratory approach of inflammation-related proteins. Patients with laparoscopy-verified endometriosis (N = 172), women with microscopic colitis (N = 50), healthy controls (N = 31), and age-matched controls from the general population (N = 100) were enrolled and questionnaires regarding socioeconomic factors, lifestyle habits, and medical history were completed. Sera from patients and healthy controls were analyzed for 92 inflammatory biomarkers using Proximity Extension Assay technology (PEA). Plasma AXIN1 levels were analyzed in patients with endometriosis and controls from the general population by ELISA. General linear model adjusted for age, Mann–Whitney U-test, and principal component analysis (PCA) were used for statistical calculations. Serum levels of AXIN1 and ST1A1 were increased in endometriosis compared with MC (p < 0.001) and healthy controls (p = 0.001), whereas CXCL9 levels were decreased. Plasma levels of AXIN1 were elevated in endometriosis compared with age-matched controls from the general population (30.0 (17.0–38.0) pg/mL vs. 19.5 (15.0–28.0) pg/mL, p < 0.001). PCA analysis identified four clusters of proteins, where one cluster differed between endometriosis and controls, with strong correlations for AXIN1 and ST1A1. Plasma/serum AXIN1 is an interesting biomarker to be further evaluated in endometriosis.
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spelling pubmed-63372382019-01-22 AXIN1 in Plasma or Serum Is a Potential New Biomarker for Endometriosis Ek, Malin Roth, Bodil Engström, Gunnar Ohlsson, Bodil Int J Mol Sci Article Although endometriosis is considered an inflammatory disease, no reliable diagnostic biomarkers exist for use in clinical practice. The aim was to investigate the inflammatory profile in endometriosis using an exploratory approach of inflammation-related proteins. Patients with laparoscopy-verified endometriosis (N = 172), women with microscopic colitis (N = 50), healthy controls (N = 31), and age-matched controls from the general population (N = 100) were enrolled and questionnaires regarding socioeconomic factors, lifestyle habits, and medical history were completed. Sera from patients and healthy controls were analyzed for 92 inflammatory biomarkers using Proximity Extension Assay technology (PEA). Plasma AXIN1 levels were analyzed in patients with endometriosis and controls from the general population by ELISA. General linear model adjusted for age, Mann–Whitney U-test, and principal component analysis (PCA) were used for statistical calculations. Serum levels of AXIN1 and ST1A1 were increased in endometriosis compared with MC (p < 0.001) and healthy controls (p = 0.001), whereas CXCL9 levels were decreased. Plasma levels of AXIN1 were elevated in endometriosis compared with age-matched controls from the general population (30.0 (17.0–38.0) pg/mL vs. 19.5 (15.0–28.0) pg/mL, p < 0.001). PCA analysis identified four clusters of proteins, where one cluster differed between endometriosis and controls, with strong correlations for AXIN1 and ST1A1. Plasma/serum AXIN1 is an interesting biomarker to be further evaluated in endometriosis. MDPI 2019-01-07 /pmc/articles/PMC6337238/ /pubmed/30621017 http://dx.doi.org/10.3390/ijms20010189 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ek, Malin
Roth, Bodil
Engström, Gunnar
Ohlsson, Bodil
AXIN1 in Plasma or Serum Is a Potential New Biomarker for Endometriosis
title AXIN1 in Plasma or Serum Is a Potential New Biomarker for Endometriosis
title_full AXIN1 in Plasma or Serum Is a Potential New Biomarker for Endometriosis
title_fullStr AXIN1 in Plasma or Serum Is a Potential New Biomarker for Endometriosis
title_full_unstemmed AXIN1 in Plasma or Serum Is a Potential New Biomarker for Endometriosis
title_short AXIN1 in Plasma or Serum Is a Potential New Biomarker for Endometriosis
title_sort axin1 in plasma or serum is a potential new biomarker for endometriosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337238/
https://www.ncbi.nlm.nih.gov/pubmed/30621017
http://dx.doi.org/10.3390/ijms20010189
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