Cargando…
Identification of Antioxidant Peptides in Enzymatic Hydrolysates of Carp (Cyprinus Carpio) Skin Gelatin
The protein by-products from carp (Cyprinus carpio) are normally discarded as industrial waste during fish processing. The objective of this study was to identify and characterise the peptides with a potential antioxidant activity that are released from carp skin proteins during hydrolysis by the Pr...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337244/ https://www.ncbi.nlm.nih.gov/pubmed/30597854 http://dx.doi.org/10.3390/molecules24010097 |
_version_ | 1783388208244981760 |
---|---|
author | Tkaczewska, Joanna Bukowski, Michal Mak, Paweł |
author_facet | Tkaczewska, Joanna Bukowski, Michal Mak, Paweł |
author_sort | Tkaczewska, Joanna |
collection | PubMed |
description | The protein by-products from carp (Cyprinus carpio) are normally discarded as industrial waste during fish processing. The objective of this study was to identify and characterise the peptides with a potential antioxidant activity that are released from carp skin proteins during hydrolysis by the Protamex enzyme mixture. This study shows that a hydrolysate of carp skin gelatin and its reversed-phase chromatography fractions have strong in vitro antioxidant properties. Among these fractions, the alanine-tyrosine (Ala-Tyr) dipeptide was identified as the major compound with high antioxidant potential. The peptide has good stability during in vitro enzymatic digestion assay and can inhibit the angiotensin-converting enzyme (ACE). In conclusion, our study proves that both the unfractionated hydrolysate of carp skin gelatin and the above-mentioned Ala-Tyr dipeptide represents attractive novel compounds for the formulation of antioxidant foods. |
format | Online Article Text |
id | pubmed-6337244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63372442019-01-25 Identification of Antioxidant Peptides in Enzymatic Hydrolysates of Carp (Cyprinus Carpio) Skin Gelatin Tkaczewska, Joanna Bukowski, Michal Mak, Paweł Molecules Article The protein by-products from carp (Cyprinus carpio) are normally discarded as industrial waste during fish processing. The objective of this study was to identify and characterise the peptides with a potential antioxidant activity that are released from carp skin proteins during hydrolysis by the Protamex enzyme mixture. This study shows that a hydrolysate of carp skin gelatin and its reversed-phase chromatography fractions have strong in vitro antioxidant properties. Among these fractions, the alanine-tyrosine (Ala-Tyr) dipeptide was identified as the major compound with high antioxidant potential. The peptide has good stability during in vitro enzymatic digestion assay and can inhibit the angiotensin-converting enzyme (ACE). In conclusion, our study proves that both the unfractionated hydrolysate of carp skin gelatin and the above-mentioned Ala-Tyr dipeptide represents attractive novel compounds for the formulation of antioxidant foods. MDPI 2018-12-28 /pmc/articles/PMC6337244/ /pubmed/30597854 http://dx.doi.org/10.3390/molecules24010097 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tkaczewska, Joanna Bukowski, Michal Mak, Paweł Identification of Antioxidant Peptides in Enzymatic Hydrolysates of Carp (Cyprinus Carpio) Skin Gelatin |
title | Identification of Antioxidant Peptides in Enzymatic Hydrolysates of Carp (Cyprinus Carpio) Skin Gelatin |
title_full | Identification of Antioxidant Peptides in Enzymatic Hydrolysates of Carp (Cyprinus Carpio) Skin Gelatin |
title_fullStr | Identification of Antioxidant Peptides in Enzymatic Hydrolysates of Carp (Cyprinus Carpio) Skin Gelatin |
title_full_unstemmed | Identification of Antioxidant Peptides in Enzymatic Hydrolysates of Carp (Cyprinus Carpio) Skin Gelatin |
title_short | Identification of Antioxidant Peptides in Enzymatic Hydrolysates of Carp (Cyprinus Carpio) Skin Gelatin |
title_sort | identification of antioxidant peptides in enzymatic hydrolysates of carp (cyprinus carpio) skin gelatin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337244/ https://www.ncbi.nlm.nih.gov/pubmed/30597854 http://dx.doi.org/10.3390/molecules24010097 |
work_keys_str_mv | AT tkaczewskajoanna identificationofantioxidantpeptidesinenzymatichydrolysatesofcarpcyprinuscarpioskingelatin AT bukowskimichal identificationofantioxidantpeptidesinenzymatichydrolysatesofcarpcyprinuscarpioskingelatin AT makpaweł identificationofantioxidantpeptidesinenzymatichydrolysatesofcarpcyprinuscarpioskingelatin |