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GmBRC1 is a Candidate Gene for Branching in Soybean (Glycine max (L.) Merrill)

Branch number is one of the main factors affecting the yield of soybean (Glycine max (L.)). In this study, we conducted a genome-wide association study combined with linkage analysis for the identification of a candidate gene controlling soybean branching. Five quantitative trait nucleotides (QTNs)...

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Autores principales: Shim, Sangrea, Ha, Jungmin, Kim, Moon Young, Choi, Man Soo, Kang, Sung-Taeg, Jeong, Soon-Chun, Moon, Jung-Kyung, Lee, Suk-Ha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337253/
https://www.ncbi.nlm.nih.gov/pubmed/30609682
http://dx.doi.org/10.3390/ijms20010135
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author Shim, Sangrea
Ha, Jungmin
Kim, Moon Young
Choi, Man Soo
Kang, Sung-Taeg
Jeong, Soon-Chun
Moon, Jung-Kyung
Lee, Suk-Ha
author_facet Shim, Sangrea
Ha, Jungmin
Kim, Moon Young
Choi, Man Soo
Kang, Sung-Taeg
Jeong, Soon-Chun
Moon, Jung-Kyung
Lee, Suk-Ha
author_sort Shim, Sangrea
collection PubMed
description Branch number is one of the main factors affecting the yield of soybean (Glycine max (L.)). In this study, we conducted a genome-wide association study combined with linkage analysis for the identification of a candidate gene controlling soybean branching. Five quantitative trait nucleotides (QTNs) were associated with branch numbers in a soybean core collection. Among these QTNs, a linkage disequilibrium (LD) block qtnBR6-1 spanning 20 genes was found to overlap a previously identified major quantitative trait locus qBR6-1. To validate and narrow down qtnBR6-1, we developed a set of near-isogenic lines (NILs) harboring high-branching (HB) and low-branching (LB) alleles of qBR6-1, with 99.96% isogenicity and different branch numbers. A cluster of single nucleotide polymorphisms (SNPs) segregating between NIL-HB and NIL-LB was located within the qtnBR6-1 LD block. Among the five genes showing differential expression between NIL-HB and NIL-LB, BRANCHED1 (BRC1; Glyma.06G210600) was down-regulated in the shoot apex of NIL-HB, and one missense mutation and two SNPs upstream of BRC1 were associated with branch numbers in 59 additional soybean accessions. BRC1 encodes TEOSINTE-BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTORS 1 and 2 transcription factor and functions as a regulatory repressor of branching. On the basis of these results, we propose BRC1 as a candidate gene for branching in soybean.
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spelling pubmed-63372532019-01-22 GmBRC1 is a Candidate Gene for Branching in Soybean (Glycine max (L.) Merrill) Shim, Sangrea Ha, Jungmin Kim, Moon Young Choi, Man Soo Kang, Sung-Taeg Jeong, Soon-Chun Moon, Jung-Kyung Lee, Suk-Ha Int J Mol Sci Article Branch number is one of the main factors affecting the yield of soybean (Glycine max (L.)). In this study, we conducted a genome-wide association study combined with linkage analysis for the identification of a candidate gene controlling soybean branching. Five quantitative trait nucleotides (QTNs) were associated with branch numbers in a soybean core collection. Among these QTNs, a linkage disequilibrium (LD) block qtnBR6-1 spanning 20 genes was found to overlap a previously identified major quantitative trait locus qBR6-1. To validate and narrow down qtnBR6-1, we developed a set of near-isogenic lines (NILs) harboring high-branching (HB) and low-branching (LB) alleles of qBR6-1, with 99.96% isogenicity and different branch numbers. A cluster of single nucleotide polymorphisms (SNPs) segregating between NIL-HB and NIL-LB was located within the qtnBR6-1 LD block. Among the five genes showing differential expression between NIL-HB and NIL-LB, BRANCHED1 (BRC1; Glyma.06G210600) was down-regulated in the shoot apex of NIL-HB, and one missense mutation and two SNPs upstream of BRC1 were associated with branch numbers in 59 additional soybean accessions. BRC1 encodes TEOSINTE-BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTORS 1 and 2 transcription factor and functions as a regulatory repressor of branching. On the basis of these results, we propose BRC1 as a candidate gene for branching in soybean. MDPI 2019-01-01 /pmc/articles/PMC6337253/ /pubmed/30609682 http://dx.doi.org/10.3390/ijms20010135 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shim, Sangrea
Ha, Jungmin
Kim, Moon Young
Choi, Man Soo
Kang, Sung-Taeg
Jeong, Soon-Chun
Moon, Jung-Kyung
Lee, Suk-Ha
GmBRC1 is a Candidate Gene for Branching in Soybean (Glycine max (L.) Merrill)
title GmBRC1 is a Candidate Gene for Branching in Soybean (Glycine max (L.) Merrill)
title_full GmBRC1 is a Candidate Gene for Branching in Soybean (Glycine max (L.) Merrill)
title_fullStr GmBRC1 is a Candidate Gene for Branching in Soybean (Glycine max (L.) Merrill)
title_full_unstemmed GmBRC1 is a Candidate Gene for Branching in Soybean (Glycine max (L.) Merrill)
title_short GmBRC1 is a Candidate Gene for Branching in Soybean (Glycine max (L.) Merrill)
title_sort gmbrc1 is a candidate gene for branching in soybean (glycine max (l.) merrill)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337253/
https://www.ncbi.nlm.nih.gov/pubmed/30609682
http://dx.doi.org/10.3390/ijms20010135
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