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ERK Activity Dynamics during Zebrafish Embryonic Development

During vertebrate development, extracellular signal-regulated kinase (ERK) is activated by growth factors such as fibroblast growth factor (FGF), and it regulates the formation of tissues/organs including eyes, brains, somites, limbs, and inner ears. However, an experimental system to monitor ERK ac...

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Autores principales: Wong, Kah-Loon, Akiyama, Ryutaro, Bessho, Yasumasa, Matsui, Takaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337290/
https://www.ncbi.nlm.nih.gov/pubmed/30597912
http://dx.doi.org/10.3390/ijms20010109
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author Wong, Kah-Loon
Akiyama, Ryutaro
Bessho, Yasumasa
Matsui, Takaaki
author_facet Wong, Kah-Loon
Akiyama, Ryutaro
Bessho, Yasumasa
Matsui, Takaaki
author_sort Wong, Kah-Loon
collection PubMed
description During vertebrate development, extracellular signal-regulated kinase (ERK) is activated by growth factors such as fibroblast growth factor (FGF), and it regulates the formation of tissues/organs including eyes, brains, somites, limbs, and inner ears. However, an experimental system to monitor ERK activity dynamics in the entire body of the vertebrate embryo is lacking. We recently studied ERK activity dynamics in the pre-somitic mesoderm of living zebrafish embryos injected with mRNAs encoding a Förster resonance energy transfer (FRET)-based ERK biosensor. In this study, transgenic zebrafish stably and ubiquitously expressing the ERK biosensor were generated to monitor ERK activity dynamics throughout embryonic development. The system allowed the identification of ERK activation domains in embryos from the late blastula to the late segmentation stage, consistent with immunostaining patterns obtained using anti-phosphorylated ERK antibody. A spatiotemporal map of ERK activity in the entire body during zebrafish embryogenesis was generated, and previously unidentified activation dynamics and ERK domains were identified. The proposed system is the first reported method to monitor ERK activity dynamics during vertebrate embryogenesis, providing insight into the role of ERK activity in normal and abnormal development in living vertebrate embryos.
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spelling pubmed-63372902019-01-22 ERK Activity Dynamics during Zebrafish Embryonic Development Wong, Kah-Loon Akiyama, Ryutaro Bessho, Yasumasa Matsui, Takaaki Int J Mol Sci Article During vertebrate development, extracellular signal-regulated kinase (ERK) is activated by growth factors such as fibroblast growth factor (FGF), and it regulates the formation of tissues/organs including eyes, brains, somites, limbs, and inner ears. However, an experimental system to monitor ERK activity dynamics in the entire body of the vertebrate embryo is lacking. We recently studied ERK activity dynamics in the pre-somitic mesoderm of living zebrafish embryos injected with mRNAs encoding a Förster resonance energy transfer (FRET)-based ERK biosensor. In this study, transgenic zebrafish stably and ubiquitously expressing the ERK biosensor were generated to monitor ERK activity dynamics throughout embryonic development. The system allowed the identification of ERK activation domains in embryos from the late blastula to the late segmentation stage, consistent with immunostaining patterns obtained using anti-phosphorylated ERK antibody. A spatiotemporal map of ERK activity in the entire body during zebrafish embryogenesis was generated, and previously unidentified activation dynamics and ERK domains were identified. The proposed system is the first reported method to monitor ERK activity dynamics during vertebrate embryogenesis, providing insight into the role of ERK activity in normal and abnormal development in living vertebrate embryos. MDPI 2018-12-28 /pmc/articles/PMC6337290/ /pubmed/30597912 http://dx.doi.org/10.3390/ijms20010109 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wong, Kah-Loon
Akiyama, Ryutaro
Bessho, Yasumasa
Matsui, Takaaki
ERK Activity Dynamics during Zebrafish Embryonic Development
title ERK Activity Dynamics during Zebrafish Embryonic Development
title_full ERK Activity Dynamics during Zebrafish Embryonic Development
title_fullStr ERK Activity Dynamics during Zebrafish Embryonic Development
title_full_unstemmed ERK Activity Dynamics during Zebrafish Embryonic Development
title_short ERK Activity Dynamics during Zebrafish Embryonic Development
title_sort erk activity dynamics during zebrafish embryonic development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337290/
https://www.ncbi.nlm.nih.gov/pubmed/30597912
http://dx.doi.org/10.3390/ijms20010109
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