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The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect
Although several new implants have been developed using animal studies for the treatment of osteochondral and cartilage defects, there is a lack of information on the possible metabolic and biochemical reactions of the body to the implantation of biomaterials and cartilage reconstruction. Therefore,...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337335/ https://www.ncbi.nlm.nih.gov/pubmed/30609876 http://dx.doi.org/10.3390/ma12010142 |
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author | Tothova, Csilla Mihajlovicova, Xenia Novotny, Jaroslav Nagy, Oskar Giretova, Maria Kresakova, Lenka Tomco, Marek Zert, Zdenek Vilhanova, Zuzana Varga, Maros Medvecky, Lubomir Petrovova, Eva |
author_facet | Tothova, Csilla Mihajlovicova, Xenia Novotny, Jaroslav Nagy, Oskar Giretova, Maria Kresakova, Lenka Tomco, Marek Zert, Zdenek Vilhanova, Zuzana Varga, Maros Medvecky, Lubomir Petrovova, Eva |
author_sort | Tothova, Csilla |
collection | PubMed |
description | Although several new implants have been developed using animal studies for the treatment of osteochondral and cartilage defects, there is a lack of information on the possible metabolic and biochemical reactions of the body to the implantation of biomaterials and cartilage reconstruction. Therefore, this study was aimed at evaluating the serum protein pattern and the alterations in the concentrations of selected acute phase proteins in five clinically healthy female sheep before and after the reconstruction of experimentally induced articular cartilage defects using polyhydroxybutyrate/chitosan based biopolymer material. The concentrations of total serum proteins (TSP), protein fractions, and selected acute phase proteins—serum amyloid A (SAA), haptoglobin (Hp), and C-reactive protein (CRP)—were measured before and on days seven, 14, and 30 after the surgical intervention. The TSP concentrations showed no marked differences during the evaluated period. Albumin values decreased on day seven and day 14 after surgery. In the concentrations of α(1)-, α(2)-, β-, and γ(2)-globulins, a gradual significant increase was observed during the postoperative period (p < 0.05). The γ(1)-globulins decreased slightly seven days after surgery. The concentrations of SAA, Hp, and CRP increased significantly after the surgical intervention with a subsequent decrease on day 30. Presented results suggest marked alterations in the serum protein pattern after surgical intervention. |
format | Online Article Text |
id | pubmed-6337335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63373352019-01-22 The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect Tothova, Csilla Mihajlovicova, Xenia Novotny, Jaroslav Nagy, Oskar Giretova, Maria Kresakova, Lenka Tomco, Marek Zert, Zdenek Vilhanova, Zuzana Varga, Maros Medvecky, Lubomir Petrovova, Eva Materials (Basel) Article Although several new implants have been developed using animal studies for the treatment of osteochondral and cartilage defects, there is a lack of information on the possible metabolic and biochemical reactions of the body to the implantation of biomaterials and cartilage reconstruction. Therefore, this study was aimed at evaluating the serum protein pattern and the alterations in the concentrations of selected acute phase proteins in five clinically healthy female sheep before and after the reconstruction of experimentally induced articular cartilage defects using polyhydroxybutyrate/chitosan based biopolymer material. The concentrations of total serum proteins (TSP), protein fractions, and selected acute phase proteins—serum amyloid A (SAA), haptoglobin (Hp), and C-reactive protein (CRP)—were measured before and on days seven, 14, and 30 after the surgical intervention. The TSP concentrations showed no marked differences during the evaluated period. Albumin values decreased on day seven and day 14 after surgery. In the concentrations of α(1)-, α(2)-, β-, and γ(2)-globulins, a gradual significant increase was observed during the postoperative period (p < 0.05). The γ(1)-globulins decreased slightly seven days after surgery. The concentrations of SAA, Hp, and CRP increased significantly after the surgical intervention with a subsequent decrease on day 30. Presented results suggest marked alterations in the serum protein pattern after surgical intervention. MDPI 2019-01-03 /pmc/articles/PMC6337335/ /pubmed/30609876 http://dx.doi.org/10.3390/ma12010142 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tothova, Csilla Mihajlovicova, Xenia Novotny, Jaroslav Nagy, Oskar Giretova, Maria Kresakova, Lenka Tomco, Marek Zert, Zdenek Vilhanova, Zuzana Varga, Maros Medvecky, Lubomir Petrovova, Eva The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect |
title | The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect |
title_full | The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect |
title_fullStr | The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect |
title_full_unstemmed | The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect |
title_short | The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect |
title_sort | serum protein profile and acute phase proteins in the postoperative period in sheep after induced articular cartilage defect |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337335/ https://www.ncbi.nlm.nih.gov/pubmed/30609876 http://dx.doi.org/10.3390/ma12010142 |
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