Cargando…

The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect

Although several new implants have been developed using animal studies for the treatment of osteochondral and cartilage defects, there is a lack of information on the possible metabolic and biochemical reactions of the body to the implantation of biomaterials and cartilage reconstruction. Therefore,...

Descripción completa

Detalles Bibliográficos
Autores principales: Tothova, Csilla, Mihajlovicova, Xenia, Novotny, Jaroslav, Nagy, Oskar, Giretova, Maria, Kresakova, Lenka, Tomco, Marek, Zert, Zdenek, Vilhanova, Zuzana, Varga, Maros, Medvecky, Lubomir, Petrovova, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337335/
https://www.ncbi.nlm.nih.gov/pubmed/30609876
http://dx.doi.org/10.3390/ma12010142
_version_ 1783388230447529984
author Tothova, Csilla
Mihajlovicova, Xenia
Novotny, Jaroslav
Nagy, Oskar
Giretova, Maria
Kresakova, Lenka
Tomco, Marek
Zert, Zdenek
Vilhanova, Zuzana
Varga, Maros
Medvecky, Lubomir
Petrovova, Eva
author_facet Tothova, Csilla
Mihajlovicova, Xenia
Novotny, Jaroslav
Nagy, Oskar
Giretova, Maria
Kresakova, Lenka
Tomco, Marek
Zert, Zdenek
Vilhanova, Zuzana
Varga, Maros
Medvecky, Lubomir
Petrovova, Eva
author_sort Tothova, Csilla
collection PubMed
description Although several new implants have been developed using animal studies for the treatment of osteochondral and cartilage defects, there is a lack of information on the possible metabolic and biochemical reactions of the body to the implantation of biomaterials and cartilage reconstruction. Therefore, this study was aimed at evaluating the serum protein pattern and the alterations in the concentrations of selected acute phase proteins in five clinically healthy female sheep before and after the reconstruction of experimentally induced articular cartilage defects using polyhydroxybutyrate/chitosan based biopolymer material. The concentrations of total serum proteins (TSP), protein fractions, and selected acute phase proteins—serum amyloid A (SAA), haptoglobin (Hp), and C-reactive protein (CRP)—were measured before and on days seven, 14, and 30 after the surgical intervention. The TSP concentrations showed no marked differences during the evaluated period. Albumin values decreased on day seven and day 14 after surgery. In the concentrations of α(1)-, α(2)-, β-, and γ(2)-globulins, a gradual significant increase was observed during the postoperative period (p < 0.05). The γ(1)-globulins decreased slightly seven days after surgery. The concentrations of SAA, Hp, and CRP increased significantly after the surgical intervention with a subsequent decrease on day 30. Presented results suggest marked alterations in the serum protein pattern after surgical intervention.
format Online
Article
Text
id pubmed-6337335
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63373352019-01-22 The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect Tothova, Csilla Mihajlovicova, Xenia Novotny, Jaroslav Nagy, Oskar Giretova, Maria Kresakova, Lenka Tomco, Marek Zert, Zdenek Vilhanova, Zuzana Varga, Maros Medvecky, Lubomir Petrovova, Eva Materials (Basel) Article Although several new implants have been developed using animal studies for the treatment of osteochondral and cartilage defects, there is a lack of information on the possible metabolic and biochemical reactions of the body to the implantation of biomaterials and cartilage reconstruction. Therefore, this study was aimed at evaluating the serum protein pattern and the alterations in the concentrations of selected acute phase proteins in five clinically healthy female sheep before and after the reconstruction of experimentally induced articular cartilage defects using polyhydroxybutyrate/chitosan based biopolymer material. The concentrations of total serum proteins (TSP), protein fractions, and selected acute phase proteins—serum amyloid A (SAA), haptoglobin (Hp), and C-reactive protein (CRP)—were measured before and on days seven, 14, and 30 after the surgical intervention. The TSP concentrations showed no marked differences during the evaluated period. Albumin values decreased on day seven and day 14 after surgery. In the concentrations of α(1)-, α(2)-, β-, and γ(2)-globulins, a gradual significant increase was observed during the postoperative period (p < 0.05). The γ(1)-globulins decreased slightly seven days after surgery. The concentrations of SAA, Hp, and CRP increased significantly after the surgical intervention with a subsequent decrease on day 30. Presented results suggest marked alterations in the serum protein pattern after surgical intervention. MDPI 2019-01-03 /pmc/articles/PMC6337335/ /pubmed/30609876 http://dx.doi.org/10.3390/ma12010142 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tothova, Csilla
Mihajlovicova, Xenia
Novotny, Jaroslav
Nagy, Oskar
Giretova, Maria
Kresakova, Lenka
Tomco, Marek
Zert, Zdenek
Vilhanova, Zuzana
Varga, Maros
Medvecky, Lubomir
Petrovova, Eva
The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect
title The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect
title_full The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect
title_fullStr The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect
title_full_unstemmed The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect
title_short The Serum Protein Profile and Acute Phase Proteins in the Postoperative Period in Sheep after Induced Articular Cartilage Defect
title_sort serum protein profile and acute phase proteins in the postoperative period in sheep after induced articular cartilage defect
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337335/
https://www.ncbi.nlm.nih.gov/pubmed/30609876
http://dx.doi.org/10.3390/ma12010142
work_keys_str_mv AT tothovacsilla theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT mihajlovicovaxenia theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT novotnyjaroslav theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT nagyoskar theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT giretovamaria theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT kresakovalenka theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT tomcomarek theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT zertzdenek theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT vilhanovazuzana theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT vargamaros theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT medveckylubomir theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT petrovovaeva theserumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT tothovacsilla serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT mihajlovicovaxenia serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT novotnyjaroslav serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT nagyoskar serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT giretovamaria serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT kresakovalenka serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT tomcomarek serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT zertzdenek serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT vilhanovazuzana serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT vargamaros serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT medveckylubomir serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect
AT petrovovaeva serumproteinprofileandacutephaseproteinsinthepostoperativeperiodinsheepafterinducedarticularcartilagedefect