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Ectopic Expression of Mulberry G-Proteins Alters Drought and Salt Stress Tolerance in Tobacco

Heterotrimeric guanine-nucleotide-binding proteins (G-proteins) play key roles in responses to various abiotic stress responses and tolerance in plants. However, the detailed mechanisms behind these roles remain unclear. Mulberry (Morus alba L.) can adapt to adverse abiotic stress conditions; howeve...

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Autores principales: Liu, Changying, Xu, Yazhen, Feng, Yang, Long, Dingpei, Cao, Boning, Xiang, Zhonghuai, Zhao, Aichun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337368/
https://www.ncbi.nlm.nih.gov/pubmed/30587818
http://dx.doi.org/10.3390/ijms20010089
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author Liu, Changying
Xu, Yazhen
Feng, Yang
Long, Dingpei
Cao, Boning
Xiang, Zhonghuai
Zhao, Aichun
author_facet Liu, Changying
Xu, Yazhen
Feng, Yang
Long, Dingpei
Cao, Boning
Xiang, Zhonghuai
Zhao, Aichun
author_sort Liu, Changying
collection PubMed
description Heterotrimeric guanine-nucleotide-binding proteins (G-proteins) play key roles in responses to various abiotic stress responses and tolerance in plants. However, the detailed mechanisms behind these roles remain unclear. Mulberry (Morus alba L.) can adapt to adverse abiotic stress conditions; however, little is known regarding the associated molecular mechanisms. In this study, mulberry G-protein genes, MaGα, MaGβ, MaGγ1, and MaGγ2, were independently transformed into tobacco, and the transgenic plants were used for resistance identification experiments. The ectopic expression of MaGα in tobacco decreased the tolerance to drought and salt stresses, while the overexpression of MaGβ, MaGγ1, and MaGγ2 increased the tolerance. Further analysis showed that mulberry G-proteins may regulate drought and salt tolerances by modulating reactive oxygen species’ detoxification. This study revealed the roles of each mulberry G-protein subunit in abiotic stress tolerance and advances our knowledge of the molecular mechanisms underlying G-proteins’ regulation of plant abiotic stress tolerance.
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spelling pubmed-63373682019-01-22 Ectopic Expression of Mulberry G-Proteins Alters Drought and Salt Stress Tolerance in Tobacco Liu, Changying Xu, Yazhen Feng, Yang Long, Dingpei Cao, Boning Xiang, Zhonghuai Zhao, Aichun Int J Mol Sci Article Heterotrimeric guanine-nucleotide-binding proteins (G-proteins) play key roles in responses to various abiotic stress responses and tolerance in plants. However, the detailed mechanisms behind these roles remain unclear. Mulberry (Morus alba L.) can adapt to adverse abiotic stress conditions; however, little is known regarding the associated molecular mechanisms. In this study, mulberry G-protein genes, MaGα, MaGβ, MaGγ1, and MaGγ2, were independently transformed into tobacco, and the transgenic plants were used for resistance identification experiments. The ectopic expression of MaGα in tobacco decreased the tolerance to drought and salt stresses, while the overexpression of MaGβ, MaGγ1, and MaGγ2 increased the tolerance. Further analysis showed that mulberry G-proteins may regulate drought and salt tolerances by modulating reactive oxygen species’ detoxification. This study revealed the roles of each mulberry G-protein subunit in abiotic stress tolerance and advances our knowledge of the molecular mechanisms underlying G-proteins’ regulation of plant abiotic stress tolerance. MDPI 2018-12-26 /pmc/articles/PMC6337368/ /pubmed/30587818 http://dx.doi.org/10.3390/ijms20010089 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Changying
Xu, Yazhen
Feng, Yang
Long, Dingpei
Cao, Boning
Xiang, Zhonghuai
Zhao, Aichun
Ectopic Expression of Mulberry G-Proteins Alters Drought and Salt Stress Tolerance in Tobacco
title Ectopic Expression of Mulberry G-Proteins Alters Drought and Salt Stress Tolerance in Tobacco
title_full Ectopic Expression of Mulberry G-Proteins Alters Drought and Salt Stress Tolerance in Tobacco
title_fullStr Ectopic Expression of Mulberry G-Proteins Alters Drought and Salt Stress Tolerance in Tobacco
title_full_unstemmed Ectopic Expression of Mulberry G-Proteins Alters Drought and Salt Stress Tolerance in Tobacco
title_short Ectopic Expression of Mulberry G-Proteins Alters Drought and Salt Stress Tolerance in Tobacco
title_sort ectopic expression of mulberry g-proteins alters drought and salt stress tolerance in tobacco
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337368/
https://www.ncbi.nlm.nih.gov/pubmed/30587818
http://dx.doi.org/10.3390/ijms20010089
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