Cargando…

Hyaluronic Acid Coated Acid-Sensitive Nanoparticles for Targeted Therapy of Adjuvant-Induced Arthritis in Rats

Activated macrophages play a vital role in rheumatoid arthritis (RA) pathophysiology. CD44 is an overexpressed receptor on activated macrophages that is a potential target site for RA treatment. In this study, we prepared hyaluronic acid (HA) coated acid-sensitive polymeric nanoparticles (HAPNPs) co...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Changhui, Li, Xiangyu, Hou, Yufei, Meng, Xiangxue, Wang, Deli, Liu, Jiaxin, Sun, Fengying, Li, Youxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337373/
https://www.ncbi.nlm.nih.gov/pubmed/30609724
http://dx.doi.org/10.3390/molecules24010146
_version_ 1783388239442214912
author Yu, Changhui
Li, Xiangyu
Hou, Yufei
Meng, Xiangxue
Wang, Deli
Liu, Jiaxin
Sun, Fengying
Li, Youxin
author_facet Yu, Changhui
Li, Xiangyu
Hou, Yufei
Meng, Xiangxue
Wang, Deli
Liu, Jiaxin
Sun, Fengying
Li, Youxin
author_sort Yu, Changhui
collection PubMed
description Activated macrophages play a vital role in rheumatoid arthritis (RA) pathophysiology. CD44 is an overexpressed receptor on activated macrophages that is a potential target site for RA treatment. In this study, we prepared hyaluronic acid (HA) coated acid-sensitive polymeric nanoparticles (HAPNPs) composed of egg phosphatidylcholine, polyethylenimine, and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) loaded with dexamethasone (Dex) for the treatment of RA. PCADK was used to form polymeric cores because of its acid-sensitivity. The HAPNPs were about 150 nm in size and had a zeta potential of −2.84 mV. The release rate of Dex from HAPNPs/Dex in vitro increased markedly when the pH decreased from 7.4 to 4.5, indicating that the HAPNPs were pH-sensitive. In a cellular uptake study, stronger fluorescence signals were observed in activated macrophages treated with HAPNPs, suggesting that HAPNPs could be effective nanodevices target to activated macrophages. In rats with adjuvant-induced arthritis, HAPNPs could inhibited the progression of RA. Taken together, these results suggest that the HAPNPs could be useful in RA therapy.
format Online
Article
Text
id pubmed-6337373
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63373732019-01-25 Hyaluronic Acid Coated Acid-Sensitive Nanoparticles for Targeted Therapy of Adjuvant-Induced Arthritis in Rats Yu, Changhui Li, Xiangyu Hou, Yufei Meng, Xiangxue Wang, Deli Liu, Jiaxin Sun, Fengying Li, Youxin Molecules Article Activated macrophages play a vital role in rheumatoid arthritis (RA) pathophysiology. CD44 is an overexpressed receptor on activated macrophages that is a potential target site for RA treatment. In this study, we prepared hyaluronic acid (HA) coated acid-sensitive polymeric nanoparticles (HAPNPs) composed of egg phosphatidylcholine, polyethylenimine, and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) loaded with dexamethasone (Dex) for the treatment of RA. PCADK was used to form polymeric cores because of its acid-sensitivity. The HAPNPs were about 150 nm in size and had a zeta potential of −2.84 mV. The release rate of Dex from HAPNPs/Dex in vitro increased markedly when the pH decreased from 7.4 to 4.5, indicating that the HAPNPs were pH-sensitive. In a cellular uptake study, stronger fluorescence signals were observed in activated macrophages treated with HAPNPs, suggesting that HAPNPs could be effective nanodevices target to activated macrophages. In rats with adjuvant-induced arthritis, HAPNPs could inhibited the progression of RA. Taken together, these results suggest that the HAPNPs could be useful in RA therapy. MDPI 2019-01-02 /pmc/articles/PMC6337373/ /pubmed/30609724 http://dx.doi.org/10.3390/molecules24010146 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Changhui
Li, Xiangyu
Hou, Yufei
Meng, Xiangxue
Wang, Deli
Liu, Jiaxin
Sun, Fengying
Li, Youxin
Hyaluronic Acid Coated Acid-Sensitive Nanoparticles for Targeted Therapy of Adjuvant-Induced Arthritis in Rats
title Hyaluronic Acid Coated Acid-Sensitive Nanoparticles for Targeted Therapy of Adjuvant-Induced Arthritis in Rats
title_full Hyaluronic Acid Coated Acid-Sensitive Nanoparticles for Targeted Therapy of Adjuvant-Induced Arthritis in Rats
title_fullStr Hyaluronic Acid Coated Acid-Sensitive Nanoparticles for Targeted Therapy of Adjuvant-Induced Arthritis in Rats
title_full_unstemmed Hyaluronic Acid Coated Acid-Sensitive Nanoparticles for Targeted Therapy of Adjuvant-Induced Arthritis in Rats
title_short Hyaluronic Acid Coated Acid-Sensitive Nanoparticles for Targeted Therapy of Adjuvant-Induced Arthritis in Rats
title_sort hyaluronic acid coated acid-sensitive nanoparticles for targeted therapy of adjuvant-induced arthritis in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337373/
https://www.ncbi.nlm.nih.gov/pubmed/30609724
http://dx.doi.org/10.3390/molecules24010146
work_keys_str_mv AT yuchanghui hyaluronicacidcoatedacidsensitivenanoparticlesfortargetedtherapyofadjuvantinducedarthritisinrats
AT lixiangyu hyaluronicacidcoatedacidsensitivenanoparticlesfortargetedtherapyofadjuvantinducedarthritisinrats
AT houyufei hyaluronicacidcoatedacidsensitivenanoparticlesfortargetedtherapyofadjuvantinducedarthritisinrats
AT mengxiangxue hyaluronicacidcoatedacidsensitivenanoparticlesfortargetedtherapyofadjuvantinducedarthritisinrats
AT wangdeli hyaluronicacidcoatedacidsensitivenanoparticlesfortargetedtherapyofadjuvantinducedarthritisinrats
AT liujiaxin hyaluronicacidcoatedacidsensitivenanoparticlesfortargetedtherapyofadjuvantinducedarthritisinrats
AT sunfengying hyaluronicacidcoatedacidsensitivenanoparticlesfortargetedtherapyofadjuvantinducedarthritisinrats
AT liyouxin hyaluronicacidcoatedacidsensitivenanoparticlesfortargetedtherapyofadjuvantinducedarthritisinrats