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Patterns of Drought Response of 38 WRKY Transcription Factors of Zanthoxylum bungeanum Maxim.
The WRKY family of transcription factors (TFs) includes a number of transcription-specific groupings that play important roles in plant growth and development and in plant responses to various stresses. To screen for WRKY transcription factors associated with drought stress in Zanthoxylum bungeanum,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337418/ https://www.ncbi.nlm.nih.gov/pubmed/30586928 http://dx.doi.org/10.3390/ijms20010068 |
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author | Fei, Xitong Hou, Lixiu Shi, Jingwei Yang, Tuxi Liu, Yulin Wei, Anzhi |
author_facet | Fei, Xitong Hou, Lixiu Shi, Jingwei Yang, Tuxi Liu, Yulin Wei, Anzhi |
author_sort | Fei, Xitong |
collection | PubMed |
description | The WRKY family of transcription factors (TFs) includes a number of transcription-specific groupings that play important roles in plant growth and development and in plant responses to various stresses. To screen for WRKY transcription factors associated with drought stress in Zanthoxylum bungeanum, a total of 38 ZbWRKY were identified and these were then classified and identified with Arabidopsis WRKY. Using bioinformatics analyses based on the structural characteristics of the conservative domain, 38 WRKY transcription factors were identified and categorized into three groups: Groups I, II, and III. Of these, Group II can be divided into four subgroups: subgroups IIb, IIc, IId, and IIe. No ZbWRKY members of subgroup IIa were found in the sequencing data. In addition, 38 ZbWRKY were identified by real-time PCR to determine the behavior of this family of genes under drought stress. Twelve ZbWRKY transcription factors were found to be significantly upregulated under drought stress and these were identified by relative quantification. As predicted by the STRING website, the results show that the WRKYs are involved in four signaling pathways—the jasmonic acid (JA), the salicylic acid (SA), the mitogen-activated protein kinase (MAPK), and the ethylene signaling pathways. ZbWRKY33 is the most intense transcription factor in response to drought stress. We predict that WRKY33 binds directly to the ethylene synthesis precursor gene ACS6, to promote ethylene synthesis. Ethylene then binds to the ethylene activator release signal to activate a series of downstream genes for cold stress and osmotic responses. The roles of ZbWRKY transcription factors in drought stress rely on a regulatory network center on the JA signaling pathway. |
format | Online Article Text |
id | pubmed-6337418 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63374182019-01-22 Patterns of Drought Response of 38 WRKY Transcription Factors of Zanthoxylum bungeanum Maxim. Fei, Xitong Hou, Lixiu Shi, Jingwei Yang, Tuxi Liu, Yulin Wei, Anzhi Int J Mol Sci Article The WRKY family of transcription factors (TFs) includes a number of transcription-specific groupings that play important roles in plant growth and development and in plant responses to various stresses. To screen for WRKY transcription factors associated with drought stress in Zanthoxylum bungeanum, a total of 38 ZbWRKY were identified and these were then classified and identified with Arabidopsis WRKY. Using bioinformatics analyses based on the structural characteristics of the conservative domain, 38 WRKY transcription factors were identified and categorized into three groups: Groups I, II, and III. Of these, Group II can be divided into four subgroups: subgroups IIb, IIc, IId, and IIe. No ZbWRKY members of subgroup IIa were found in the sequencing data. In addition, 38 ZbWRKY were identified by real-time PCR to determine the behavior of this family of genes under drought stress. Twelve ZbWRKY transcription factors were found to be significantly upregulated under drought stress and these were identified by relative quantification. As predicted by the STRING website, the results show that the WRKYs are involved in four signaling pathways—the jasmonic acid (JA), the salicylic acid (SA), the mitogen-activated protein kinase (MAPK), and the ethylene signaling pathways. ZbWRKY33 is the most intense transcription factor in response to drought stress. We predict that WRKY33 binds directly to the ethylene synthesis precursor gene ACS6, to promote ethylene synthesis. Ethylene then binds to the ethylene activator release signal to activate a series of downstream genes for cold stress and osmotic responses. The roles of ZbWRKY transcription factors in drought stress rely on a regulatory network center on the JA signaling pathway. MDPI 2018-12-24 /pmc/articles/PMC6337418/ /pubmed/30586928 http://dx.doi.org/10.3390/ijms20010068 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fei, Xitong Hou, Lixiu Shi, Jingwei Yang, Tuxi Liu, Yulin Wei, Anzhi Patterns of Drought Response of 38 WRKY Transcription Factors of Zanthoxylum bungeanum Maxim. |
title | Patterns of Drought Response of 38 WRKY Transcription Factors of Zanthoxylum bungeanum Maxim. |
title_full | Patterns of Drought Response of 38 WRKY Transcription Factors of Zanthoxylum bungeanum Maxim. |
title_fullStr | Patterns of Drought Response of 38 WRKY Transcription Factors of Zanthoxylum bungeanum Maxim. |
title_full_unstemmed | Patterns of Drought Response of 38 WRKY Transcription Factors of Zanthoxylum bungeanum Maxim. |
title_short | Patterns of Drought Response of 38 WRKY Transcription Factors of Zanthoxylum bungeanum Maxim. |
title_sort | patterns of drought response of 38 wrky transcription factors of zanthoxylum bungeanum maxim. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337418/ https://www.ncbi.nlm.nih.gov/pubmed/30586928 http://dx.doi.org/10.3390/ijms20010068 |
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