Cargando…
Recent Advances and New Perspectives in Capillary Electrophoresis-Mass Spectrometry for Single Cell “Omics”
Accurate clinical therapeutics rely on understanding the metabolic responses of individual cells. However, the high level of heterogeneity between cells means that simply sampling from large populations of cells is not necessarily a reliable approximation of an individual cell’s response. As a resul...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337428/ https://www.ncbi.nlm.nih.gov/pubmed/30583525 http://dx.doi.org/10.3390/molecules24010042 |
_version_ | 1783388252397371392 |
---|---|
author | DeLaney, Kellen Sauer, Christopher S. Vu, Nhu Q. Li, Lingjun |
author_facet | DeLaney, Kellen Sauer, Christopher S. Vu, Nhu Q. Li, Lingjun |
author_sort | DeLaney, Kellen |
collection | PubMed |
description | Accurate clinical therapeutics rely on understanding the metabolic responses of individual cells. However, the high level of heterogeneity between cells means that simply sampling from large populations of cells is not necessarily a reliable approximation of an individual cell’s response. As a result, there have been numerous developments in the field of single-cell analysis to address this lack of knowledge. Many of these developments have focused on the coupling of capillary electrophoresis (CE), a separation technique with low sample consumption and high resolving power, and mass spectrometry (MS), a sensitive detection method for interrogating all ions in a sample in a single analysis. In recent years, there have been many notable advancements at each step of the single-cell CE-MS analysis workflow, including sampling, manipulation, separation, and MS analysis. In each of these areas, the combined improvements in analytical instrumentation and achievements of numerous researchers have served to drive the field forward to new frontiers. Consequently, notable biological discoveries have been made possible by the implementation of these methods. Although there is still room in the field for numerous further advances, researchers have effectively minimized various limitations in detection of analytes, and it is expected that there will be many more developments in the near future. |
format | Online Article Text |
id | pubmed-6337428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63374282019-01-25 Recent Advances and New Perspectives in Capillary Electrophoresis-Mass Spectrometry for Single Cell “Omics” DeLaney, Kellen Sauer, Christopher S. Vu, Nhu Q. Li, Lingjun Molecules Review Accurate clinical therapeutics rely on understanding the metabolic responses of individual cells. However, the high level of heterogeneity between cells means that simply sampling from large populations of cells is not necessarily a reliable approximation of an individual cell’s response. As a result, there have been numerous developments in the field of single-cell analysis to address this lack of knowledge. Many of these developments have focused on the coupling of capillary electrophoresis (CE), a separation technique with low sample consumption and high resolving power, and mass spectrometry (MS), a sensitive detection method for interrogating all ions in a sample in a single analysis. In recent years, there have been many notable advancements at each step of the single-cell CE-MS analysis workflow, including sampling, manipulation, separation, and MS analysis. In each of these areas, the combined improvements in analytical instrumentation and achievements of numerous researchers have served to drive the field forward to new frontiers. Consequently, notable biological discoveries have been made possible by the implementation of these methods. Although there is still room in the field for numerous further advances, researchers have effectively minimized various limitations in detection of analytes, and it is expected that there will be many more developments in the near future. MDPI 2018-12-22 /pmc/articles/PMC6337428/ /pubmed/30583525 http://dx.doi.org/10.3390/molecules24010042 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review DeLaney, Kellen Sauer, Christopher S. Vu, Nhu Q. Li, Lingjun Recent Advances and New Perspectives in Capillary Electrophoresis-Mass Spectrometry for Single Cell “Omics” |
title | Recent Advances and New Perspectives in Capillary Electrophoresis-Mass Spectrometry for Single Cell “Omics” |
title_full | Recent Advances and New Perspectives in Capillary Electrophoresis-Mass Spectrometry for Single Cell “Omics” |
title_fullStr | Recent Advances and New Perspectives in Capillary Electrophoresis-Mass Spectrometry for Single Cell “Omics” |
title_full_unstemmed | Recent Advances and New Perspectives in Capillary Electrophoresis-Mass Spectrometry for Single Cell “Omics” |
title_short | Recent Advances and New Perspectives in Capillary Electrophoresis-Mass Spectrometry for Single Cell “Omics” |
title_sort | recent advances and new perspectives in capillary electrophoresis-mass spectrometry for single cell “omics” |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337428/ https://www.ncbi.nlm.nih.gov/pubmed/30583525 http://dx.doi.org/10.3390/molecules24010042 |
work_keys_str_mv | AT delaneykellen recentadvancesandnewperspectivesincapillaryelectrophoresismassspectrometryforsinglecellomics AT sauerchristophers recentadvancesandnewperspectivesincapillaryelectrophoresismassspectrometryforsinglecellomics AT vunhuq recentadvancesandnewperspectivesincapillaryelectrophoresismassspectrometryforsinglecellomics AT lilingjun recentadvancesandnewperspectivesincapillaryelectrophoresismassspectrometryforsinglecellomics |