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Increasing Uptake of Silica Nanoparticles with Electroporation: From Cellular Characterization to Potential Applications
In the fields of biology and medicine, nanoproducts such as nanoparticles (NPs) are specifically interesting as theranostic tools, since they offer the double capacity to locally deliver active drugs and to image exactly where the product is delivered. Among the many described possibilities, silica...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337455/ https://www.ncbi.nlm.nih.gov/pubmed/30621089 http://dx.doi.org/10.3390/ma12010179 |
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author | Phonesouk, Erick Lechevallier, Séverine Ferrand, Audrey Rols, Marie-Pierre Bezombes, Christine Verelst, Marc Golzio, Muriel |
author_facet | Phonesouk, Erick Lechevallier, Séverine Ferrand, Audrey Rols, Marie-Pierre Bezombes, Christine Verelst, Marc Golzio, Muriel |
author_sort | Phonesouk, Erick |
collection | PubMed |
description | In the fields of biology and medicine, nanoproducts such as nanoparticles (NPs) are specifically interesting as theranostic tools, since they offer the double capacity to locally deliver active drugs and to image exactly where the product is delivered. Among the many described possibilities, silica nanoparticles (SiNPs) represent a good choice because of their ease of synthesis, the possibility of their vast functionalization, and their good biocompatibility. However, SiNPs’ passive cell internalization by endocytosis only distributes NPs into the cell cytoplasm and is unable to target the nucleus if SiNPs are larger than a few nanometers. In this study, we demonstrate that the cell penetration of SiNPs of 28–30 nm in diameter can be strongly enhanced using a physical method, called electroporation or electropermeabilization (EP). The uptake of fluorescently labelled silica nanoparticles was improved in two different cancer cell lines, namely, HCT-116 (human colon cancer) cells and RL (B-lymphoma) cells. First, we studied cells’ capability for the regular passive uptake of SiNPs in vitro. Then, we set EP parameters in order to induce a more efficient and rapid cell loading, also comprising the nuclear compartment, while preserving the cell viability. In the final approach, we performed in vivo experiments, and evidenced that the labeling was long-lasting, as confirmed by fluorescence imaging of labeled tumors, which enabled a 30-day follow-up. This kind of SiNPs delivery, achieved by EP, could be employed to load extensive amounts of active ingredients into the cell nucleus, and concomitantly allow the monitoring of the long-term fate of nanoparticles. |
format | Online Article Text |
id | pubmed-6337455 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63374552019-01-22 Increasing Uptake of Silica Nanoparticles with Electroporation: From Cellular Characterization to Potential Applications Phonesouk, Erick Lechevallier, Séverine Ferrand, Audrey Rols, Marie-Pierre Bezombes, Christine Verelst, Marc Golzio, Muriel Materials (Basel) Article In the fields of biology and medicine, nanoproducts such as nanoparticles (NPs) are specifically interesting as theranostic tools, since they offer the double capacity to locally deliver active drugs and to image exactly where the product is delivered. Among the many described possibilities, silica nanoparticles (SiNPs) represent a good choice because of their ease of synthesis, the possibility of their vast functionalization, and their good biocompatibility. However, SiNPs’ passive cell internalization by endocytosis only distributes NPs into the cell cytoplasm and is unable to target the nucleus if SiNPs are larger than a few nanometers. In this study, we demonstrate that the cell penetration of SiNPs of 28–30 nm in diameter can be strongly enhanced using a physical method, called electroporation or electropermeabilization (EP). The uptake of fluorescently labelled silica nanoparticles was improved in two different cancer cell lines, namely, HCT-116 (human colon cancer) cells and RL (B-lymphoma) cells. First, we studied cells’ capability for the regular passive uptake of SiNPs in vitro. Then, we set EP parameters in order to induce a more efficient and rapid cell loading, also comprising the nuclear compartment, while preserving the cell viability. In the final approach, we performed in vivo experiments, and evidenced that the labeling was long-lasting, as confirmed by fluorescence imaging of labeled tumors, which enabled a 30-day follow-up. This kind of SiNPs delivery, achieved by EP, could be employed to load extensive amounts of active ingredients into the cell nucleus, and concomitantly allow the monitoring of the long-term fate of nanoparticles. MDPI 2019-01-07 /pmc/articles/PMC6337455/ /pubmed/30621089 http://dx.doi.org/10.3390/ma12010179 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Phonesouk, Erick Lechevallier, Séverine Ferrand, Audrey Rols, Marie-Pierre Bezombes, Christine Verelst, Marc Golzio, Muriel Increasing Uptake of Silica Nanoparticles with Electroporation: From Cellular Characterization to Potential Applications |
title | Increasing Uptake of Silica Nanoparticles with Electroporation: From Cellular Characterization to Potential Applications |
title_full | Increasing Uptake of Silica Nanoparticles with Electroporation: From Cellular Characterization to Potential Applications |
title_fullStr | Increasing Uptake of Silica Nanoparticles with Electroporation: From Cellular Characterization to Potential Applications |
title_full_unstemmed | Increasing Uptake of Silica Nanoparticles with Electroporation: From Cellular Characterization to Potential Applications |
title_short | Increasing Uptake of Silica Nanoparticles with Electroporation: From Cellular Characterization to Potential Applications |
title_sort | increasing uptake of silica nanoparticles with electroporation: from cellular characterization to potential applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337455/ https://www.ncbi.nlm.nih.gov/pubmed/30621089 http://dx.doi.org/10.3390/ma12010179 |
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