The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells

The role of the epithelial-mesenchymal transition (EMT) in ovarian cancer cell progression is unquestioned. In this report, we describe that malignant ascites, fluid that accumulates in the peritoneal cavity in a large group of patients with ovarian cancer, stimulate EMT in two representative ovaria...

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Autores principales: Pakuła, Martyna, Mikuła-Pietrasik, Justyna, Witucka, Anna, Kostka-Jeziorny, Katarzyna, Uruski, Paweł, Moszyński, Rafał, Naumowicz, Eryk, Sajdak, Stefan, Tykarski, Andrzej, Książek, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337462/
https://www.ncbi.nlm.nih.gov/pubmed/30609691
http://dx.doi.org/10.3390/ijms20010137
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author Pakuła, Martyna
Mikuła-Pietrasik, Justyna
Witucka, Anna
Kostka-Jeziorny, Katarzyna
Uruski, Paweł
Moszyński, Rafał
Naumowicz, Eryk
Sajdak, Stefan
Tykarski, Andrzej
Książek, Krzysztof
author_facet Pakuła, Martyna
Mikuła-Pietrasik, Justyna
Witucka, Anna
Kostka-Jeziorny, Katarzyna
Uruski, Paweł
Moszyński, Rafał
Naumowicz, Eryk
Sajdak, Stefan
Tykarski, Andrzej
Książek, Krzysztof
author_sort Pakuła, Martyna
collection PubMed
description The role of the epithelial-mesenchymal transition (EMT) in ovarian cancer cell progression is unquestioned. In this report, we describe that malignant ascites, fluid that accumulates in the peritoneal cavity in a large group of patients with ovarian cancer, stimulate EMT in two representative ovarian cancer cell lines (A2780, SKOV-3). In addition, we identify the ascites-derived mediators of EMT and signaling pathways initiated in the cancer cells that underlie this phenomenon. Finally, we demonstrate that EMT induced in the cancer cells in response to the malignant ascites contributes to their increased transmesothelial invasion. Altogether, our study provides new insight into the mechanistic aspects of the malignant ascites-dependent exacerbation of the intraperitoneal progression of ovarian cancer.
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spelling pubmed-63374622019-01-22 The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells Pakuła, Martyna Mikuła-Pietrasik, Justyna Witucka, Anna Kostka-Jeziorny, Katarzyna Uruski, Paweł Moszyński, Rafał Naumowicz, Eryk Sajdak, Stefan Tykarski, Andrzej Książek, Krzysztof Int J Mol Sci Article The role of the epithelial-mesenchymal transition (EMT) in ovarian cancer cell progression is unquestioned. In this report, we describe that malignant ascites, fluid that accumulates in the peritoneal cavity in a large group of patients with ovarian cancer, stimulate EMT in two representative ovarian cancer cell lines (A2780, SKOV-3). In addition, we identify the ascites-derived mediators of EMT and signaling pathways initiated in the cancer cells that underlie this phenomenon. Finally, we demonstrate that EMT induced in the cancer cells in response to the malignant ascites contributes to their increased transmesothelial invasion. Altogether, our study provides new insight into the mechanistic aspects of the malignant ascites-dependent exacerbation of the intraperitoneal progression of ovarian cancer. MDPI 2019-01-02 /pmc/articles/PMC6337462/ /pubmed/30609691 http://dx.doi.org/10.3390/ijms20010137 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pakuła, Martyna
Mikuła-Pietrasik, Justyna
Witucka, Anna
Kostka-Jeziorny, Katarzyna
Uruski, Paweł
Moszyński, Rafał
Naumowicz, Eryk
Sajdak, Stefan
Tykarski, Andrzej
Książek, Krzysztof
The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
title The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
title_full The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
title_fullStr The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
title_full_unstemmed The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
title_short The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
title_sort epithelial-mesenchymal transition initiated by malignant ascites underlies the transmesothelial invasion of ovarian cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337462/
https://www.ncbi.nlm.nih.gov/pubmed/30609691
http://dx.doi.org/10.3390/ijms20010137
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