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UPLC-QTOF/MS-Based Nontargeted Metabolomic Analysis of Mountain- and Garden-Cultivated Ginseng of Different Ages in Northeast China
Aiming at further systematically comparing the similarities and differences of the chemical components in ginseng of different ages, especially comparing the younger or the older and mountain-cultivated ginseng (MCG), 4, 5, 6-year-old cultivated ginseng (CG) and 12, 20-year-old MCG were chosen as th...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337476/ https://www.ncbi.nlm.nih.gov/pubmed/30583458 http://dx.doi.org/10.3390/molecules24010033 |
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author | Zhu, Hailin Lin, Hongqiang Tan, Jing Wang, Cuizhu Wang, Han Wu, Fulin Dong, Qinghai Liu, Yunhe Li, Pingya Liu, Jinping |
author_facet | Zhu, Hailin Lin, Hongqiang Tan, Jing Wang, Cuizhu Wang, Han Wu, Fulin Dong, Qinghai Liu, Yunhe Li, Pingya Liu, Jinping |
author_sort | Zhu, Hailin |
collection | PubMed |
description | Aiming at further systematically comparing the similarities and differences of the chemical components in ginseng of different ages, especially comparing the younger or the older and mountain-cultivated ginseng (MCG), 4, 5, 6-year-old cultivated ginseng (CG) and 12, 20-year-old MCG were chosen as the analytical samples in the present study. The combination of UPLC-QTOF-MS(E), UNIFI platform and multivariate statistical analysis were developed to profile CGs and MCGs. By the screening analysis based on UNIFI, 126 chemical components with various structural types were characterized or tentatively identified from all the CG and MCG samples for the first time. The results showed that all the CG and MCG samples had the similar chemical composition, but there were significant differences in the contents of markers. By the metabolomic analysis based on multivariate statistical analysis, it was shown that CG(4–6 years), MCG(12 years) and MCG(20 years) samples were obviously divided into three different groups, and a total of 17 potential age-dependent markers enabling differentiation among the three groups of samples were discovered. For differentiation from other two kinds of samples, there were four robust makers such as α-linolenic acid, 9-octadecenoic acid, linoleic acid and panaxydol for CG(4–6 years), five robust makers including ginsenoside Re(1), -Re(2), -Rs(1), malonylginsenoside Rb(2) and isomer of malonylginsenoside Rb(1) for MCG(20 years), and two robust makers, 24-hydroxyoleanolic acid and palmitoleic acid, for MCG(12 years) were discovered, respectively. The proposed approach could be applied to directly distinguish MCG root ages, which is an important criterion for evaluating the quality of MCG. The results will provide the data for the further study on the chemical constituents of MCG. |
format | Online Article Text |
id | pubmed-6337476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63374762019-01-25 UPLC-QTOF/MS-Based Nontargeted Metabolomic Analysis of Mountain- and Garden-Cultivated Ginseng of Different Ages in Northeast China Zhu, Hailin Lin, Hongqiang Tan, Jing Wang, Cuizhu Wang, Han Wu, Fulin Dong, Qinghai Liu, Yunhe Li, Pingya Liu, Jinping Molecules Article Aiming at further systematically comparing the similarities and differences of the chemical components in ginseng of different ages, especially comparing the younger or the older and mountain-cultivated ginseng (MCG), 4, 5, 6-year-old cultivated ginseng (CG) and 12, 20-year-old MCG were chosen as the analytical samples in the present study. The combination of UPLC-QTOF-MS(E), UNIFI platform and multivariate statistical analysis were developed to profile CGs and MCGs. By the screening analysis based on UNIFI, 126 chemical components with various structural types were characterized or tentatively identified from all the CG and MCG samples for the first time. The results showed that all the CG and MCG samples had the similar chemical composition, but there were significant differences in the contents of markers. By the metabolomic analysis based on multivariate statistical analysis, it was shown that CG(4–6 years), MCG(12 years) and MCG(20 years) samples were obviously divided into three different groups, and a total of 17 potential age-dependent markers enabling differentiation among the three groups of samples were discovered. For differentiation from other two kinds of samples, there were four robust makers such as α-linolenic acid, 9-octadecenoic acid, linoleic acid and panaxydol for CG(4–6 years), five robust makers including ginsenoside Re(1), -Re(2), -Rs(1), malonylginsenoside Rb(2) and isomer of malonylginsenoside Rb(1) for MCG(20 years), and two robust makers, 24-hydroxyoleanolic acid and palmitoleic acid, for MCG(12 years) were discovered, respectively. The proposed approach could be applied to directly distinguish MCG root ages, which is an important criterion for evaluating the quality of MCG. The results will provide the data for the further study on the chemical constituents of MCG. MDPI 2018-12-21 /pmc/articles/PMC6337476/ /pubmed/30583458 http://dx.doi.org/10.3390/molecules24010033 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhu, Hailin Lin, Hongqiang Tan, Jing Wang, Cuizhu Wang, Han Wu, Fulin Dong, Qinghai Liu, Yunhe Li, Pingya Liu, Jinping UPLC-QTOF/MS-Based Nontargeted Metabolomic Analysis of Mountain- and Garden-Cultivated Ginseng of Different Ages in Northeast China |
title | UPLC-QTOF/MS-Based Nontargeted Metabolomic Analysis of Mountain- and Garden-Cultivated Ginseng of Different Ages in Northeast China |
title_full | UPLC-QTOF/MS-Based Nontargeted Metabolomic Analysis of Mountain- and Garden-Cultivated Ginseng of Different Ages in Northeast China |
title_fullStr | UPLC-QTOF/MS-Based Nontargeted Metabolomic Analysis of Mountain- and Garden-Cultivated Ginseng of Different Ages in Northeast China |
title_full_unstemmed | UPLC-QTOF/MS-Based Nontargeted Metabolomic Analysis of Mountain- and Garden-Cultivated Ginseng of Different Ages in Northeast China |
title_short | UPLC-QTOF/MS-Based Nontargeted Metabolomic Analysis of Mountain- and Garden-Cultivated Ginseng of Different Ages in Northeast China |
title_sort | uplc-qtof/ms-based nontargeted metabolomic analysis of mountain- and garden-cultivated ginseng of different ages in northeast china |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337476/ https://www.ncbi.nlm.nih.gov/pubmed/30583458 http://dx.doi.org/10.3390/molecules24010033 |
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