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Hydrogen Peroxide and Nitric Oxide Crosstalk Mediates Brassinosteroids Induced Cold Stress Tolerance in Medicago truncatula

Brassinosteroids (BRs) play pivotal roles in modulating plant growth, development, and stress responses. In this study, a Medicago truncatula plant pretreated with brassinolide (BL, the most active BR), enhanced cold stress tolerance by regulating the expression of several cold-related genes and ant...

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Autores principales: Arfan, Muhammad, Zhang, Da-Wei, Zou, Li-Juan, Luo, Shi-Shuai, Tan, Wen-Rong, Zhu, Tong, Lin, Hong-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337477/
https://www.ncbi.nlm.nih.gov/pubmed/30609774
http://dx.doi.org/10.3390/ijms20010144
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author Arfan, Muhammad
Zhang, Da-Wei
Zou, Li-Juan
Luo, Shi-Shuai
Tan, Wen-Rong
Zhu, Tong
Lin, Hong-Hui
author_facet Arfan, Muhammad
Zhang, Da-Wei
Zou, Li-Juan
Luo, Shi-Shuai
Tan, Wen-Rong
Zhu, Tong
Lin, Hong-Hui
author_sort Arfan, Muhammad
collection PubMed
description Brassinosteroids (BRs) play pivotal roles in modulating plant growth, development, and stress responses. In this study, a Medicago truncatula plant pretreated with brassinolide (BL, the most active BR), enhanced cold stress tolerance by regulating the expression of several cold-related genes and antioxidant enzymes activities. Previous studies reported that hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) are involved during environmental stress conditions. However, how these two signaling molecules interact with each other in BRs-induced abiotic stress tolerance remain largely unclear. BL-pretreatment induced, while brassinazole (BRZ, a specific inhibitor of BRs biosynthesis) reduced H(2)O(2) and NO production. Further, application of dimethylthiourea (DMTU, a H(2)O(2) and OH(−) scavenger) blocked BRs-induced NO production, but BRs-induced H(2)O(2) generation was not sensitive to 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO, a scavenger of NO). Moreover, pretreatment with DMTU and PTIO decreased BL-induced mitochondrial alternative oxidase (AOX) and the photosystem capacity. However, pretreatment with PTIO was found to be more effective than DMTU in reducing BRs-induced increases in V(alt), V(t), and MtAOX1 gene expression. Similarly, BRs-induced photosystem II efficiency was found in NO dependent manner than H(2)O(2). Finally, we conclude that H(2)O(2) was involved in NO generation, whereas NO was found to be crucial in BRs-induced AOX capacity, which further contributed to the protection of the photosystem under cold stress conditions in Medicago truncatula.
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spelling pubmed-63374772019-01-22 Hydrogen Peroxide and Nitric Oxide Crosstalk Mediates Brassinosteroids Induced Cold Stress Tolerance in Medicago truncatula Arfan, Muhammad Zhang, Da-Wei Zou, Li-Juan Luo, Shi-Shuai Tan, Wen-Rong Zhu, Tong Lin, Hong-Hui Int J Mol Sci Article Brassinosteroids (BRs) play pivotal roles in modulating plant growth, development, and stress responses. In this study, a Medicago truncatula plant pretreated with brassinolide (BL, the most active BR), enhanced cold stress tolerance by regulating the expression of several cold-related genes and antioxidant enzymes activities. Previous studies reported that hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) are involved during environmental stress conditions. However, how these two signaling molecules interact with each other in BRs-induced abiotic stress tolerance remain largely unclear. BL-pretreatment induced, while brassinazole (BRZ, a specific inhibitor of BRs biosynthesis) reduced H(2)O(2) and NO production. Further, application of dimethylthiourea (DMTU, a H(2)O(2) and OH(−) scavenger) blocked BRs-induced NO production, but BRs-induced H(2)O(2) generation was not sensitive to 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO, a scavenger of NO). Moreover, pretreatment with DMTU and PTIO decreased BL-induced mitochondrial alternative oxidase (AOX) and the photosystem capacity. However, pretreatment with PTIO was found to be more effective than DMTU in reducing BRs-induced increases in V(alt), V(t), and MtAOX1 gene expression. Similarly, BRs-induced photosystem II efficiency was found in NO dependent manner than H(2)O(2). Finally, we conclude that H(2)O(2) was involved in NO generation, whereas NO was found to be crucial in BRs-induced AOX capacity, which further contributed to the protection of the photosystem under cold stress conditions in Medicago truncatula. MDPI 2019-01-02 /pmc/articles/PMC6337477/ /pubmed/30609774 http://dx.doi.org/10.3390/ijms20010144 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arfan, Muhammad
Zhang, Da-Wei
Zou, Li-Juan
Luo, Shi-Shuai
Tan, Wen-Rong
Zhu, Tong
Lin, Hong-Hui
Hydrogen Peroxide and Nitric Oxide Crosstalk Mediates Brassinosteroids Induced Cold Stress Tolerance in Medicago truncatula
title Hydrogen Peroxide and Nitric Oxide Crosstalk Mediates Brassinosteroids Induced Cold Stress Tolerance in Medicago truncatula
title_full Hydrogen Peroxide and Nitric Oxide Crosstalk Mediates Brassinosteroids Induced Cold Stress Tolerance in Medicago truncatula
title_fullStr Hydrogen Peroxide and Nitric Oxide Crosstalk Mediates Brassinosteroids Induced Cold Stress Tolerance in Medicago truncatula
title_full_unstemmed Hydrogen Peroxide and Nitric Oxide Crosstalk Mediates Brassinosteroids Induced Cold Stress Tolerance in Medicago truncatula
title_short Hydrogen Peroxide and Nitric Oxide Crosstalk Mediates Brassinosteroids Induced Cold Stress Tolerance in Medicago truncatula
title_sort hydrogen peroxide and nitric oxide crosstalk mediates brassinosteroids induced cold stress tolerance in medicago truncatula
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337477/
https://www.ncbi.nlm.nih.gov/pubmed/30609774
http://dx.doi.org/10.3390/ijms20010144
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