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Unique Biological Activity and Potential Role of Monomeric Laminin-γ2 as a Novel Biomarker for Hepatocellular Carcinoma: A Review

Laminin (Ln)-332 consists of α3, β3, and γ2 chains, which mediate epithelial cell adhesion to the basement membrane. Ln-γ2, a component of Ln-332, is frequently expressed as a monomer in the invasion front of several types of malignant tissues without simultaneous expression of Ln-α3 and/or Ln-β3 ch...

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Autores principales: Yasuda, Hiroshi, Nakagawa, Masatoshi, Kiyokawa, Hirofumi, Yoshida, Eisaku, Yoshimura, Toru, Koshikawa, Naohiko, Itoh, Fumio, Seiki, Motoharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337480/
https://www.ncbi.nlm.nih.gov/pubmed/30626121
http://dx.doi.org/10.3390/ijms20010226
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author Yasuda, Hiroshi
Nakagawa, Masatoshi
Kiyokawa, Hirofumi
Yoshida, Eisaku
Yoshimura, Toru
Koshikawa, Naohiko
Itoh, Fumio
Seiki, Motoharu
author_facet Yasuda, Hiroshi
Nakagawa, Masatoshi
Kiyokawa, Hirofumi
Yoshida, Eisaku
Yoshimura, Toru
Koshikawa, Naohiko
Itoh, Fumio
Seiki, Motoharu
author_sort Yasuda, Hiroshi
collection PubMed
description Laminin (Ln)-332 consists of α3, β3, and γ2 chains, which mediate epithelial cell adhesion to the basement membrane. Ln-γ2, a component of Ln-332, is frequently expressed as a monomer in the invasion front of several types of malignant tissues without simultaneous expression of Ln-α3 and/or Ln-β3 chains. Moreover, monomeric Ln-γ2 induces tumor cell proliferation and migration in vitro. These unique biological activities indicate that monomeric Ln-γ2 could be a candidate biomarker for early cancer surveillance. However, the present immune method for monomeric Ln-γ2 detection can only predict its expression, since no antibody that specifically reacts with monomeric γ2, but not with heterotrimeric γ2 chain, is commercially available. We have, therefore, developed monoclonal antibodies to specifically detect monomeric Ln-γ2, and devised a highly sensitive method to measure serum monomeric Ln-γ2 levels using a fully automated chemiluminescent immunoassay (CLIA). We evaluated its diagnostic value in sera from patients with several digestive cancers, including hepatocellular carcinoma (HCC), and found serum monomeric Ln-γ2 to be a clinically available biomarker for HCC surveillance. The combination of monomeric Ln-γ2 and prothrombin induced by Vitamin K Absence II (PIVKA-II) may be more sensitive for clinical diagnosis of HCC than any currently used combination.
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spelling pubmed-63374802019-01-22 Unique Biological Activity and Potential Role of Monomeric Laminin-γ2 as a Novel Biomarker for Hepatocellular Carcinoma: A Review Yasuda, Hiroshi Nakagawa, Masatoshi Kiyokawa, Hirofumi Yoshida, Eisaku Yoshimura, Toru Koshikawa, Naohiko Itoh, Fumio Seiki, Motoharu Int J Mol Sci Review Laminin (Ln)-332 consists of α3, β3, and γ2 chains, which mediate epithelial cell adhesion to the basement membrane. Ln-γ2, a component of Ln-332, is frequently expressed as a monomer in the invasion front of several types of malignant tissues without simultaneous expression of Ln-α3 and/or Ln-β3 chains. Moreover, monomeric Ln-γ2 induces tumor cell proliferation and migration in vitro. These unique biological activities indicate that monomeric Ln-γ2 could be a candidate biomarker for early cancer surveillance. However, the present immune method for monomeric Ln-γ2 detection can only predict its expression, since no antibody that specifically reacts with monomeric γ2, but not with heterotrimeric γ2 chain, is commercially available. We have, therefore, developed monoclonal antibodies to specifically detect monomeric Ln-γ2, and devised a highly sensitive method to measure serum monomeric Ln-γ2 levels using a fully automated chemiluminescent immunoassay (CLIA). We evaluated its diagnostic value in sera from patients with several digestive cancers, including hepatocellular carcinoma (HCC), and found serum monomeric Ln-γ2 to be a clinically available biomarker for HCC surveillance. The combination of monomeric Ln-γ2 and prothrombin induced by Vitamin K Absence II (PIVKA-II) may be more sensitive for clinical diagnosis of HCC than any currently used combination. MDPI 2019-01-08 /pmc/articles/PMC6337480/ /pubmed/30626121 http://dx.doi.org/10.3390/ijms20010226 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Yasuda, Hiroshi
Nakagawa, Masatoshi
Kiyokawa, Hirofumi
Yoshida, Eisaku
Yoshimura, Toru
Koshikawa, Naohiko
Itoh, Fumio
Seiki, Motoharu
Unique Biological Activity and Potential Role of Monomeric Laminin-γ2 as a Novel Biomarker for Hepatocellular Carcinoma: A Review
title Unique Biological Activity and Potential Role of Monomeric Laminin-γ2 as a Novel Biomarker for Hepatocellular Carcinoma: A Review
title_full Unique Biological Activity and Potential Role of Monomeric Laminin-γ2 as a Novel Biomarker for Hepatocellular Carcinoma: A Review
title_fullStr Unique Biological Activity and Potential Role of Monomeric Laminin-γ2 as a Novel Biomarker for Hepatocellular Carcinoma: A Review
title_full_unstemmed Unique Biological Activity and Potential Role of Monomeric Laminin-γ2 as a Novel Biomarker for Hepatocellular Carcinoma: A Review
title_short Unique Biological Activity and Potential Role of Monomeric Laminin-γ2 as a Novel Biomarker for Hepatocellular Carcinoma: A Review
title_sort unique biological activity and potential role of monomeric laminin-γ2 as a novel biomarker for hepatocellular carcinoma: a review
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337480/
https://www.ncbi.nlm.nih.gov/pubmed/30626121
http://dx.doi.org/10.3390/ijms20010226
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