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Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line

Ephedra alata Decne. belongs to the Ephedraceae family. It is a species of Ephedra that grows mostly in the desert. Today, the main importance of Ephedra species in the medical field is due to the presence of the alkaloids derived from phenyl-alanine, which act on the sympathetic nervous system as a...

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Autores principales: Danciu, Corina, Muntean, Delia, Alexa, Ersilia, Farcas, Claudia, Oprean, Camelia, Zupko, Istvan, Bor, Andrea, Minda, Daliana, Proks, Maria, Buda, Valentina, Hancianu, Monica, Cioanca, Oana, Soica, Codruta, Popescu, Sofia, Dehelean, Cristina Adriana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337526/
https://www.ncbi.nlm.nih.gov/pubmed/30577537
http://dx.doi.org/10.3390/molecules24010013
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author Danciu, Corina
Muntean, Delia
Alexa, Ersilia
Farcas, Claudia
Oprean, Camelia
Zupko, Istvan
Bor, Andrea
Minda, Daliana
Proks, Maria
Buda, Valentina
Hancianu, Monica
Cioanca, Oana
Soica, Codruta
Popescu, Sofia
Dehelean, Cristina Adriana
author_facet Danciu, Corina
Muntean, Delia
Alexa, Ersilia
Farcas, Claudia
Oprean, Camelia
Zupko, Istvan
Bor, Andrea
Minda, Daliana
Proks, Maria
Buda, Valentina
Hancianu, Monica
Cioanca, Oana
Soica, Codruta
Popescu, Sofia
Dehelean, Cristina Adriana
author_sort Danciu, Corina
collection PubMed
description Ephedra alata Decne. belongs to the Ephedraceae family. It is a species of Ephedra that grows mostly in the desert. Today, the main importance of Ephedra species in the medical field is due to the presence of the alkaloids derived from phenyl-alanine, which act on the sympathetic nervous system as a sympathomimetic. The aim of this study was to conduct a phytochemical characterization of the hydroalcoholic extract of the aerial part of Ephedra alata Decne., which is indigenous to Tunis, that involves the total phenolic content, individual phenolic content, and antioxidant activity as well as a biological screening for the evaluation of the antimicrobial, antifungal, antiproliferative, pro-apoptotic, and cytotoxic potential against the MCF-7 breast cancer cell line. The results show that the hydroalcoholic extract contains polyphenolic phytocompounds (156.226 ± 0.5 mgGAE/g extract) and elicits antioxidant activity (7453.18 ± 2.5 μmol Trolox/g extract). The extract acted as a bacteriostatic agent against all tested bacterial strains, but was bactericidal only against the Gram-positive cocci and Candida spp. In the set experimental parameters, the extract presents antiproliferative, pro-apoptotic, and cytotoxic potential against the MCF-7 human breast cancer cell line.
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spelling pubmed-63375262019-01-25 Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line Danciu, Corina Muntean, Delia Alexa, Ersilia Farcas, Claudia Oprean, Camelia Zupko, Istvan Bor, Andrea Minda, Daliana Proks, Maria Buda, Valentina Hancianu, Monica Cioanca, Oana Soica, Codruta Popescu, Sofia Dehelean, Cristina Adriana Molecules Article Ephedra alata Decne. belongs to the Ephedraceae family. It is a species of Ephedra that grows mostly in the desert. Today, the main importance of Ephedra species in the medical field is due to the presence of the alkaloids derived from phenyl-alanine, which act on the sympathetic nervous system as a sympathomimetic. The aim of this study was to conduct a phytochemical characterization of the hydroalcoholic extract of the aerial part of Ephedra alata Decne., which is indigenous to Tunis, that involves the total phenolic content, individual phenolic content, and antioxidant activity as well as a biological screening for the evaluation of the antimicrobial, antifungal, antiproliferative, pro-apoptotic, and cytotoxic potential against the MCF-7 breast cancer cell line. The results show that the hydroalcoholic extract contains polyphenolic phytocompounds (156.226 ± 0.5 mgGAE/g extract) and elicits antioxidant activity (7453.18 ± 2.5 μmol Trolox/g extract). The extract acted as a bacteriostatic agent against all tested bacterial strains, but was bactericidal only against the Gram-positive cocci and Candida spp. In the set experimental parameters, the extract presents antiproliferative, pro-apoptotic, and cytotoxic potential against the MCF-7 human breast cancer cell line. MDPI 2018-12-20 /pmc/articles/PMC6337526/ /pubmed/30577537 http://dx.doi.org/10.3390/molecules24010013 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Danciu, Corina
Muntean, Delia
Alexa, Ersilia
Farcas, Claudia
Oprean, Camelia
Zupko, Istvan
Bor, Andrea
Minda, Daliana
Proks, Maria
Buda, Valentina
Hancianu, Monica
Cioanca, Oana
Soica, Codruta
Popescu, Sofia
Dehelean, Cristina Adriana
Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line
title Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line
title_full Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line
title_fullStr Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line
title_full_unstemmed Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line
title_short Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line
title_sort phytochemical characterization and evaluation of the antimicrobial, antiproliferative and pro-apoptotic potential of ephedra alata decne. hydroalcoholic extract against the mcf-7 breast cancer cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337526/
https://www.ncbi.nlm.nih.gov/pubmed/30577537
http://dx.doi.org/10.3390/molecules24010013
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