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Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection
Major depressive disorder is now becoming a common disease in daily life, and most patients do not have satisfactory treatment outcomes. We herein evaluated the therapeutic effects of Zhile capsule and clarified the molecular mechanism. A rat model of chronic unpredictable mild stress-induced depres...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337550/ https://www.ncbi.nlm.nih.gov/pubmed/30625977 http://dx.doi.org/10.3390/ijms20010195 |
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author | Wu, Jiawei Zhang, Tingting Yu, Luping Huang, Shuai Yang, Yu Yu, Suyun Li, Jun Cao, Yuzhu Wei, Zhonghong Li, Xiaoman Wu, Yuanyuan Chen, Wenxing Wang, Aiyun Lu, Yin |
author_facet | Wu, Jiawei Zhang, Tingting Yu, Luping Huang, Shuai Yang, Yu Yu, Suyun Li, Jun Cao, Yuzhu Wei, Zhonghong Li, Xiaoman Wu, Yuanyuan Chen, Wenxing Wang, Aiyun Lu, Yin |
author_sort | Wu, Jiawei |
collection | PubMed |
description | Major depressive disorder is now becoming a common disease in daily life, and most patients do not have satisfactory treatment outcomes. We herein evaluated the therapeutic effects of Zhile capsule and clarified the molecular mechanism. A rat model of chronic unpredictable mild stress-induced depression was established to assess the antidepressant-like effects of Zhile by using the sucrose preference test, open field test, forced swim test, tail suspension test and HPLC. Systems pharmacology was then performed to unravel the underlying mechanism which was confirmed by western blot, enzyme-linked immunosorbent assay, and qPCR. Zhile alleviated depression-like behaviors by upregulating the cAMP-CREB-BDNF (brain-derived neurotrophic factor) axis to exert neuroprotective effects. It may be beneficial to depressive patients in clinical practice. |
format | Online Article Text |
id | pubmed-6337550 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63375502019-01-22 Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection Wu, Jiawei Zhang, Tingting Yu, Luping Huang, Shuai Yang, Yu Yu, Suyun Li, Jun Cao, Yuzhu Wei, Zhonghong Li, Xiaoman Wu, Yuanyuan Chen, Wenxing Wang, Aiyun Lu, Yin Int J Mol Sci Article Major depressive disorder is now becoming a common disease in daily life, and most patients do not have satisfactory treatment outcomes. We herein evaluated the therapeutic effects of Zhile capsule and clarified the molecular mechanism. A rat model of chronic unpredictable mild stress-induced depression was established to assess the antidepressant-like effects of Zhile by using the sucrose preference test, open field test, forced swim test, tail suspension test and HPLC. Systems pharmacology was then performed to unravel the underlying mechanism which was confirmed by western blot, enzyme-linked immunosorbent assay, and qPCR. Zhile alleviated depression-like behaviors by upregulating the cAMP-CREB-BDNF (brain-derived neurotrophic factor) axis to exert neuroprotective effects. It may be beneficial to depressive patients in clinical practice. MDPI 2019-01-08 /pmc/articles/PMC6337550/ /pubmed/30625977 http://dx.doi.org/10.3390/ijms20010195 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wu, Jiawei Zhang, Tingting Yu, Luping Huang, Shuai Yang, Yu Yu, Suyun Li, Jun Cao, Yuzhu Wei, Zhonghong Li, Xiaoman Wu, Yuanyuan Chen, Wenxing Wang, Aiyun Lu, Yin Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection |
title | Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection |
title_full | Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection |
title_fullStr | Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection |
title_full_unstemmed | Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection |
title_short | Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection |
title_sort | zhile capsule exerts antidepressant-like effects through upregulation of the bdnf signaling pathway and neuroprotection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337550/ https://www.ncbi.nlm.nih.gov/pubmed/30625977 http://dx.doi.org/10.3390/ijms20010195 |
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