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Identification and Characterization of the EXO70 Gene Family in Polyploid Wheat and Related Species

The EXO70 gene family is involved in different biological processes in plants, ranging from plant polar growth to plant immunity. To date, analysis of the EXO70 gene family has been limited in Triticeae species, e.g., hexaploidy Triticum aestivum and its ancestral/related species. By in silico analy...

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Autores principales: Zhao, Jia, Zhang, Xu, Wan, Wentao, Zhang, Heng, Liu, Jia, Li, Mengli, Wang, Haiyan, Xiao, Jin, Wang, Xiue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337732/
https://www.ncbi.nlm.nih.gov/pubmed/30586859
http://dx.doi.org/10.3390/ijms20010060
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author Zhao, Jia
Zhang, Xu
Wan, Wentao
Zhang, Heng
Liu, Jia
Li, Mengli
Wang, Haiyan
Xiao, Jin
Wang, Xiue
author_facet Zhao, Jia
Zhang, Xu
Wan, Wentao
Zhang, Heng
Liu, Jia
Li, Mengli
Wang, Haiyan
Xiao, Jin
Wang, Xiue
author_sort Zhao, Jia
collection PubMed
description The EXO70 gene family is involved in different biological processes in plants, ranging from plant polar growth to plant immunity. To date, analysis of the EXO70 gene family has been limited in Triticeae species, e.g., hexaploidy Triticum aestivum and its ancestral/related species. By in silico analysis of multiple Triticeae sequence databases, a total of 200 EXO70 members were identified. By homologue cloning approaches, 15 full-length cDNA of EXO70s were cloned from diploid Haynaldia villosa. Phylogenetic relationship analysis of 215 EXO70 members classified them into three groups (EXO70.1, EXO70.2, and EXO70.3) and nine subgroups (EXO70A to EXO70I). The distribution of most EXO70 genes among different species/sub-genomes were collinear, implying their orthologous relationship. The EXO70A subgroup has the most introns (at least five introns), while the remaining seven subgroups have only one intron on average. The expression profiling of EXO70 genes from wheat revealed that 40 wheat EXO70 genes were expressed in at least one tissue (leaf, stem, or root), of which 25 wheat EXO70 genes were in response to at least one biotic stress (stripe rust or powdery mildew) or abiotic stress (drought or heat). Subcellular localization analysis showed that ten EXO70-V proteins had distinct plasma membrane localization, EXO70I1-V showed a distinctive spotted pattern on the membrane. The 15 EXO70-V genes were differentially expressed in three tissue. Apart from EXO70D2-V, the remaining EXO70-V genes were in response to at least one stress (flg22, chitin, powdery mildew, drought, NaCl, heat, or cold) or phytohormones (salicylic acid, methyl jasmonate, ethephon, or abscisic acid) and hydrogen peroxide treatments. This research provides a genome-wide glimpse of the Triticeae EXO70 gene family and those up- or downregulated genes require further validation of their biological roles in response to biotic/abiotic stresses.
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spelling pubmed-63377322019-01-22 Identification and Characterization of the EXO70 Gene Family in Polyploid Wheat and Related Species Zhao, Jia Zhang, Xu Wan, Wentao Zhang, Heng Liu, Jia Li, Mengli Wang, Haiyan Xiao, Jin Wang, Xiue Int J Mol Sci Article The EXO70 gene family is involved in different biological processes in plants, ranging from plant polar growth to plant immunity. To date, analysis of the EXO70 gene family has been limited in Triticeae species, e.g., hexaploidy Triticum aestivum and its ancestral/related species. By in silico analysis of multiple Triticeae sequence databases, a total of 200 EXO70 members were identified. By homologue cloning approaches, 15 full-length cDNA of EXO70s were cloned from diploid Haynaldia villosa. Phylogenetic relationship analysis of 215 EXO70 members classified them into three groups (EXO70.1, EXO70.2, and EXO70.3) and nine subgroups (EXO70A to EXO70I). The distribution of most EXO70 genes among different species/sub-genomes were collinear, implying their orthologous relationship. The EXO70A subgroup has the most introns (at least five introns), while the remaining seven subgroups have only one intron on average. The expression profiling of EXO70 genes from wheat revealed that 40 wheat EXO70 genes were expressed in at least one tissue (leaf, stem, or root), of which 25 wheat EXO70 genes were in response to at least one biotic stress (stripe rust or powdery mildew) or abiotic stress (drought or heat). Subcellular localization analysis showed that ten EXO70-V proteins had distinct plasma membrane localization, EXO70I1-V showed a distinctive spotted pattern on the membrane. The 15 EXO70-V genes were differentially expressed in three tissue. Apart from EXO70D2-V, the remaining EXO70-V genes were in response to at least one stress (flg22, chitin, powdery mildew, drought, NaCl, heat, or cold) or phytohormones (salicylic acid, methyl jasmonate, ethephon, or abscisic acid) and hydrogen peroxide treatments. This research provides a genome-wide glimpse of the Triticeae EXO70 gene family and those up- or downregulated genes require further validation of their biological roles in response to biotic/abiotic stresses. MDPI 2018-12-24 /pmc/articles/PMC6337732/ /pubmed/30586859 http://dx.doi.org/10.3390/ijms20010060 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Jia
Zhang, Xu
Wan, Wentao
Zhang, Heng
Liu, Jia
Li, Mengli
Wang, Haiyan
Xiao, Jin
Wang, Xiue
Identification and Characterization of the EXO70 Gene Family in Polyploid Wheat and Related Species
title Identification and Characterization of the EXO70 Gene Family in Polyploid Wheat and Related Species
title_full Identification and Characterization of the EXO70 Gene Family in Polyploid Wheat and Related Species
title_fullStr Identification and Characterization of the EXO70 Gene Family in Polyploid Wheat and Related Species
title_full_unstemmed Identification and Characterization of the EXO70 Gene Family in Polyploid Wheat and Related Species
title_short Identification and Characterization of the EXO70 Gene Family in Polyploid Wheat and Related Species
title_sort identification and characterization of the exo70 gene family in polyploid wheat and related species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337732/
https://www.ncbi.nlm.nih.gov/pubmed/30586859
http://dx.doi.org/10.3390/ijms20010060
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